Product List
PPP1R3B Antibody
The PPP1R3B polyclonal antibody is meticulously produced in rabbits using recombinant human protein phosphatase 1 regulatory subunit 3B protein (amino acids 1-285) as the immunogen. Protein G affinity chromatography is employed to purify the antibody, achieving a purity level exceeding 95%. This rigorous purification process ensures the antibody's high quality and freedom from impurities that could potentially interfere with experimental outcomes.
This PPP1R3B antibody is capable of detecting PPP1R3B protein in human, mouse, and rat samples. Its versatility has been extensively validated in diverse applications, including ELISA, Western blotting (WB), and immunohistochemistry (IHC). These applications enable both qualitative and quantitative detection of PPP1R3B protein, facilitating the determination of its presence, size, localization, and expression levels within biological samples.
LCT Antibody
The LCT antibody is designed to specifically recognize the human LCT protein. A rabbit was immunized with recombinant human LCT protein (amino acids 983-1252). This immunization elicited an immune response in the rabbit, leading to the production of a high concentration of antibodies against the LCT protein. The rabbit's blood was collected and processed to isolate the anti-serum, which was then purified using protein G to obtain the LCT polyclonal antibody. This antibody exhibits a purity of 95%+ and has been validated for its specificity in ELISA and IHC applications.
LCT is an enzyme responsible for the breakdown of lactose into glucose and galactose within the small intestine. Consequently, the primary function of the LCT protein is the digestion of lactose, a sugar prevalent in milk and dairy products. This digestive process provides the body with a readily accessible source of energy and essential nutrients. A deficiency in lactase activity can lead to lactose intolerance, which can manifest as digestive symptoms such as bloating, gas, and diarrhea.
VP1 Antibody
This Rabbit anti-Chicken anemia virus VP1 Polyclonal antibody has been validated for use in ELISA and Western blot applications. The non-conjugated IgG has been purified using protein G, achieving a purity level of up to 95%. It exhibits reactivity with VP1 proteins from both chicken and avian anemia virus origins, making it suitable for detecting endogenous levels of the VP1 protein.
Introduction
Polyclonal antibodies are a diverse group of antibodies produced by different B cell clones in the body. They recognize and bind to multiple epitopes on a single antigen. Unlike monoclonal antibodies, which are derived from a single B cell clone and recognize a single epitope, polyclonal antibodies are heterogeneous and can target various sites on an antigen. They are classified based on their source (e.g., rabbit, goat, mouse) and the type of antigen they target (e.g., proteins, peptides, small molecules).
Key Biological Properties: Polyclonal antibodies are characterized by their ability to recognize multiple epitopes, which enhances their binding strength and specificity. They are typically produced in response to an antigenic stimulus and can be found in the serum of immunized animals.
Expression Patterns: Polyclonal antibodies are produced by B cells in response to antigen exposure. The expression patterns depend on the immunization protocol and the animal species used.
Tissue Distribution: These antibodies are primarily found in the blood serum but can also be present in other body fluids and tissues, depending on the immune response and the distribution of the antigen.
Primary Biological Functions: Polyclonal antibodies play a crucial role in the immune system by recognizing and neutralizing pathogens, such as bacteria and viruses. They facilitate the clearance of antigens through various immune mechanisms, including opsonization, complement activation, and antibody-dependent cellular cytotoxicity (ADCC).
Role in Immune Responses: Polyclonal antibodies are essential for the adaptive immune response. They provide a broad and robust defense against pathogens by targeting multiple epitopes, which reduces the likelihood of immune evasion by the pathogen.
Pathogen Recognition: These antibodies recognize and bind to specific antigens on the surface of pathogens, marking them for destruction by other immune cells.
Mechanisms with Other Molecules and Cells: Polyclonal antibodies interact with various immune cells, such as macrophages, neutrophils, and natural killer (NK) cells, to mediate immune responses. They can also bind to Fc receptors on immune cells, enhancing phagocytosis and cytotoxicity.
Binding Partners: The primary binding partners of polyclonal antibodies are antigens, which can be proteins, peptides, or other molecules. They can also interact with complement proteins and Fc receptors.
Downstream Signaling Cascades: Upon binding to their target antigens, polyclonal antibodies can trigger downstream signaling cascades that lead to the activation of immune responses. This includes the activation of the complement system, which enhances opsonization and lysis of pathogens.
Expression and Activity Control: The production and activity of polyclonal antibodies are regulated by various factors, including the nature of the antigen, the immunization protocol, and the host’s immune system.
Transcriptional Regulation: The expression of polyclonal antibodies is controlled at the transcriptional level by cytokines and other signaling molecules that influence B cell activation and differentiation.
Post-Translational Modifications: Polyclonal antibodies can undergo post-translational modifications, such as glycosylation, which can affect their stability, binding affinity, and effector functions.
Biomedical Research: Polyclonal antibodies are widely used in research for detecting and quantifying proteins, studying protein-protein interactions, and investigating cellular pathways.
Diagnostic Tools: They are used in various diagnostic assays, such as ELISA, Western blotting, and immunohistochemistry, to detect the presence of specific antigens in samples.
Therapeutic Strategies: Polyclonal antibodies are used in therapeutic applications, such as antivenoms, immunoglobulin replacement therapy, and passive immunization against infectious diseases.
Development: Polyclonal antibodies are produced throughout an individual’s life in response to antigen exposure. They play a critical role in the development of the immune system by providing protection against pathogens.
Aging: As individuals age, the production and diversity of polyclonal antibodies may decline, leading to a reduced ability to respond to new antigens and an increased susceptibility to infections.
Disease: Polyclonal antibodies are involved in various diseases, including autoimmune disorders, where they may target self-antigens, and infectious diseases, where they provide protection against pathogens.
