Product List
SIGF Antibody
The SIGF polyclonal antibody is generated by immunizing a rabbit with a recombinant Arabidopsis thaliana SIGF protein segment (amino acids 296-547). Multiple repeated immunizations elicit an immune response in the rabbit, resulting in the production of polyclonal antibodies specifically targeting SIGF. The collected rabbit serum, containing these polyclonal antibodies, undergoes purification using affinity chromatography. The functionality of the SIGF antibody is rigorously assessed through ELISA and WB assays, confirming its capability to reliably detect the Arabidopsis thaliana SIGF protein in experimental settings.
SEC14L3 Antibody
This antibody against SEC14L3 was generated in rabbits through immunization with recombinant human SEC14L3 protein, specifically targeting the amino acid sequence spanning positions 572 to 744. The isolated antibody underwent purification using protein G affinity chromatography, achieving a purity level exceeding 95%.
This SEC14L3 antibody exhibits the ability to recognize and bind SEC14L3 protein from both human and mouse samples. Its efficacy has been validated in multiple applications, including ELISA, Western blotting (WB), and immunohistochemistry (IHC). Successful validation in these assays highlights its effectiveness in detecting and identifying SEC14L3 protein.
CCR10 Antibody
This polyclonal antibody is generated by immunizing rabbits with the recombinant human C-C chemokine receptor type 10 (CCR10) protein (314-362AA). The antibody is unconjugated IgG, purified by protein G, and exhibits a purity of up to 95%. It specifically recognizes human CCR10 protein. This antibody has been validated for use in ELISA, Western Blot (WB), Immunohistochemistry (IHC), and Immunofluorescence (IF) assays.
CCR10 is a receptor for CCL27 and CCL28. The interaction of CCR10 with CCL27 is implicated in T cell-mediated skin inflammation, while CCR10/CCL28 interactions are crucial for the efficient accumulation of antigen-specific IgA plasma cells in the murine large intestine and mammary gland.
Ets21C Antibody
This Ets21C polyclonal antibody is generated using a recombinant Drosophila melanogaster (Fruit fly) Ets21C protein (amino acids 165-334) as the immunogen in a rabbit. The resulting serum contains polyclonal antibodies, which are purified through affinity chromatography. The antibody's ability to detect the Drosophila melanogaster (Fruit fly) Ets21C protein is validated using ELISA and WB assays, confirming its suitability for research applications.
Drosophila melanogaster Ets21C protein is involved in regulating gene expression during developmental processes and in response to environmental stimuli. Ets21C, a transcriptional activator, binds to DNA and recruits other proteins to the gene promoter. This recruitment process facilitates the assembly of the transcriptional machinery, enabling gene transcription. Ets21C also plays a role in regulating gene expression through post-translational modifications and interactions with other proteins.
Introduction
Polyclonal antibodies are a diverse group of antibodies produced by different B cell clones in the body. They recognize and bind to multiple epitopes on a single antigen. Unlike monoclonal antibodies, which are derived from a single B cell clone and recognize a single epitope, polyclonal antibodies are heterogeneous and can target various sites on an antigen. They are classified based on their source (e.g., rabbit, goat, mouse) and the type of antigen they target (e.g., proteins, peptides, small molecules).
Key Biological Properties: Polyclonal antibodies are characterized by their ability to recognize multiple epitopes, which enhances their binding strength and specificity. They are typically produced in response to an antigenic stimulus and can be found in the serum of immunized animals.
Expression Patterns: Polyclonal antibodies are produced by B cells in response to antigen exposure. The expression patterns depend on the immunization protocol and the animal species used.
Tissue Distribution: These antibodies are primarily found in the blood serum but can also be present in other body fluids and tissues, depending on the immune response and the distribution of the antigen.
Primary Biological Functions: Polyclonal antibodies play a crucial role in the immune system by recognizing and neutralizing pathogens, such as bacteria and viruses. They facilitate the clearance of antigens through various immune mechanisms, including opsonization, complement activation, and antibody-dependent cellular cytotoxicity (ADCC).
Role in Immune Responses: Polyclonal antibodies are essential for the adaptive immune response. They provide a broad and robust defense against pathogens by targeting multiple epitopes, which reduces the likelihood of immune evasion by the pathogen.
Pathogen Recognition: These antibodies recognize and bind to specific antigens on the surface of pathogens, marking them for destruction by other immune cells.
Mechanisms with Other Molecules and Cells: Polyclonal antibodies interact with various immune cells, such as macrophages, neutrophils, and natural killer (NK) cells, to mediate immune responses. They can also bind to Fc receptors on immune cells, enhancing phagocytosis and cytotoxicity.
Binding Partners: The primary binding partners of polyclonal antibodies are antigens, which can be proteins, peptides, or other molecules. They can also interact with complement proteins and Fc receptors.
Downstream Signaling Cascades: Upon binding to their target antigens, polyclonal antibodies can trigger downstream signaling cascades that lead to the activation of immune responses. This includes the activation of the complement system, which enhances opsonization and lysis of pathogens.
Expression and Activity Control: The production and activity of polyclonal antibodies are regulated by various factors, including the nature of the antigen, the immunization protocol, and the host’s immune system.
Transcriptional Regulation: The expression of polyclonal antibodies is controlled at the transcriptional level by cytokines and other signaling molecules that influence B cell activation and differentiation.
Post-Translational Modifications: Polyclonal antibodies can undergo post-translational modifications, such as glycosylation, which can affect their stability, binding affinity, and effector functions.
Biomedical Research: Polyclonal antibodies are widely used in research for detecting and quantifying proteins, studying protein-protein interactions, and investigating cellular pathways.
Diagnostic Tools: They are used in various diagnostic assays, such as ELISA, Western blotting, and immunohistochemistry, to detect the presence of specific antigens in samples.
Therapeutic Strategies: Polyclonal antibodies are used in therapeutic applications, such as antivenoms, immunoglobulin replacement therapy, and passive immunization against infectious diseases.
Development: Polyclonal antibodies are produced throughout an individual’s life in response to antigen exposure. They play a critical role in the development of the immune system by providing protection against pathogens.
Aging: As individuals age, the production and diversity of polyclonal antibodies may decline, leading to a reduced ability to respond to new antigens and an increased susceptibility to infections.
Disease: Polyclonal antibodies are involved in various diseases, including autoimmune disorders, where they may target self-antigens, and infectious diseases, where they provide protection against pathogens.
