Product List
PDLP7 Antibody
The PDLP7 polyclonal antibody is generated using a recombinant Arabidopsis thaliana PDLP7 protein (amino acids 31-298) as the immunogen. This protein is used to immunize a rabbit, leading to the production of antibodies. The PDLP7 antibody is then extracted and purified from the rabbit serum through affinity chromatography. This antibody is suitable for detecting Arabidopsis thaliana PDLP7 protein in ELISA and Western blot assays.
Research suggests that PDLP7 plays a crucial role in regulating plasmodesmata, specialized channels facilitating communication between plant cells. PDLP7 may control the transport of molecules and signals through these channels, thereby impacting various physiological processes in plants.
At5g63020 Antibody
The At5g63020 polyclonal antibody is developed by immunizing a rabbit with a recombinant Arabidopsis thaliana At5g63020 protein (amino acids 360-450). This immunization process stimulates an antibody response in the rabbit. Subsequently, the serum containing the polyclonal antibodies is collected and purified using affinity chromatography. The antibody's ability to detect Arabidopsis thaliana At5g63020 protein is validated through ELISA and Western blot assays.
GIF1 Antibody
This GIF1 polyclonal antibody is generated using a recombinant Arabidopsis thaliana GIF1 protein segment (comprising amino acids 1-210) as the immunogen. This immunogen is administered to a rabbit to elicit an antibody response. Serum collected from the immunized rabbit contains polyclonal antibodies, which are then purified through affinity chromatography. The functionality of this GIF1 antibody is validated using ELISA and Western blot (WB) assays, confirming its ability to effectively detect Arabidopsis thaliana GIF1 protein in experimental settings.
Arabidopsis thaliana GIF1 protein is a transcription factor that plays a critical role in regulating plant growth and development. Specifically, it has been demonstrated to be involved in the regulation of root elongation and gravitropism, which is the plant's ability to sense and respond to gravity. GIF1 also contributes to the regulation of flowering time and seed germination.
Introduction
Polyclonal antibodies are a diverse group of antibodies produced by different B cell clones in the body. They recognize and bind to multiple epitopes on a single antigen. Unlike monoclonal antibodies, which are derived from a single B cell clone and recognize a single epitope, polyclonal antibodies are heterogeneous and can target various sites on an antigen. They are classified based on their source (e.g., rabbit, goat, mouse) and the type of antigen they target (e.g., proteins, peptides, small molecules).
Key Biological Properties: Polyclonal antibodies are characterized by their ability to recognize multiple epitopes, which enhances their binding strength and specificity. They are typically produced in response to an antigenic stimulus and can be found in the serum of immunized animals.
Expression Patterns: Polyclonal antibodies are produced by B cells in response to antigen exposure. The expression patterns depend on the immunization protocol and the animal species used.
Tissue Distribution: These antibodies are primarily found in the blood serum but can also be present in other body fluids and tissues, depending on the immune response and the distribution of the antigen.
Primary Biological Functions: Polyclonal antibodies play a crucial role in the immune system by recognizing and neutralizing pathogens, such as bacteria and viruses. They facilitate the clearance of antigens through various immune mechanisms, including opsonization, complement activation, and antibody-dependent cellular cytotoxicity (ADCC).
Role in Immune Responses: Polyclonal antibodies are essential for the adaptive immune response. They provide a broad and robust defense against pathogens by targeting multiple epitopes, which reduces the likelihood of immune evasion by the pathogen.
Pathogen Recognition: These antibodies recognize and bind to specific antigens on the surface of pathogens, marking them for destruction by other immune cells.
Mechanisms with Other Molecules and Cells: Polyclonal antibodies interact with various immune cells, such as macrophages, neutrophils, and natural killer (NK) cells, to mediate immune responses. They can also bind to Fc receptors on immune cells, enhancing phagocytosis and cytotoxicity.
Binding Partners: The primary binding partners of polyclonal antibodies are antigens, which can be proteins, peptides, or other molecules. They can also interact with complement proteins and Fc receptors.
Downstream Signaling Cascades: Upon binding to their target antigens, polyclonal antibodies can trigger downstream signaling cascades that lead to the activation of immune responses. This includes the activation of the complement system, which enhances opsonization and lysis of pathogens.
Expression and Activity Control: The production and activity of polyclonal antibodies are regulated by various factors, including the nature of the antigen, the immunization protocol, and the host’s immune system.
Transcriptional Regulation: The expression of polyclonal antibodies is controlled at the transcriptional level by cytokines and other signaling molecules that influence B cell activation and differentiation.
Post-Translational Modifications: Polyclonal antibodies can undergo post-translational modifications, such as glycosylation, which can affect their stability, binding affinity, and effector functions.
Biomedical Research: Polyclonal antibodies are widely used in research for detecting and quantifying proteins, studying protein-protein interactions, and investigating cellular pathways.
Diagnostic Tools: They are used in various diagnostic assays, such as ELISA, Western blotting, and immunohistochemistry, to detect the presence of specific antigens in samples.
Therapeutic Strategies: Polyclonal antibodies are used in therapeutic applications, such as antivenoms, immunoglobulin replacement therapy, and passive immunization against infectious diseases.
Development: Polyclonal antibodies are produced throughout an individual’s life in response to antigen exposure. They play a critical role in the development of the immune system by providing protection against pathogens.
Aging: As individuals age, the production and diversity of polyclonal antibodies may decline, leading to a reduced ability to respond to new antigens and an increased susceptibility to infections.
Disease: Polyclonal antibodies are involved in various diseases, including autoimmune disorders, where they may target self-antigens, and infectious diseases, where they provide protection against pathogens.
