PGAM1 Mouse

Phosphoglycerate Mutase 1 Mouse Recombinant

Shipped with Ice Packs

In Stock

Cat. No.

BT12669

Source

Escherichia Coli.

Synonyms

Phosphoglycerate mutase 1, BPG-dependent PGAM 1, Phosphoglycerate mutase isozyme B, PGAM-B, Pgam1, Pgam-1, 2310050F24Rik.

Appearance

Sterile Filtered clear colorless solution.

Purity

Greater than 95% as determined by SDS-PAGE.

Usage

THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Quick Inquiry

Description

Biochemical Characterization of PGAM1 in Mice

PGAM1 is a dimeric enzyme that exists in isoforms (e.g., brain (BB), muscle (MM), and hybrid (MB) forms) and is essential for glycolysis. Key biochemical features include:

Parameter	Details	Source
Catalytic Function	Reversible conversion of 3-PG to 2-PG in glycolysis.
Isoforms	Tissue-specific expression (e.g., brain vs. muscle isoforms).
Post-Translational Role	Interacts with 2,3-bisphosphoglycerate (2,3-BPG) to modulate activity.
Enzyme Activity	>150 units/mg (specific activity in recombinant mouse PGAM1).

Recombinant PGAM1 proteins (e.g., His-tagged versions expressed in E. coli) are widely used in biochemical assays to study glycolytic flux and metabolic reprogramming .

Genetic Models of Pgam1 in Mice

Genetic knockout (KO) and heterozygous (Pgam1+/−) models have revealed PGAM1’s essential role in development and metabolism.

Table 1: Genetic Models of Pgam1 in Mice

Genotype	Phenotype	Key Findings
Homozygous KO	Embryonic lethality.	No viable Pgam1−/− mice survive beyond embryonic stages.
Heterozygous KO	Viable, with 50% reduction in Pgam1 mRNA and enzymatic activity.	Normal glucose tolerance; no significant changes in Pgam2 expression.
T Cell-Specific	Impaired T cell proliferation, differentiation, and immune responses.	Reduced glycolytic intermediates and TCA cycle metabolites in CD8+ T cells.

Immune Function

PGAM1 is critical for T cell activation. Pgam1 deletion in T cells leads to:

Reduced glycolysis: Impaired conversion of 3-PG to 2-PG, limiting energy production during activation .
Impaired differentiation: Defective Th1/Th17 responses, as seen in experimental autoimmune encephalomyelitis (EAE) models .
Diminished inflammation: Reduced eosinophil/neutrophil infiltration in allergic airway inflammation models .

Reproductive Biology

In Sertoli cells (SCs), PGAM1 regulates spermatogonial stem cell (SSC) fate:

PGAM1 overexpression: Upregulates genes promoting SSC proliferation.
PGAM1 knockdown: Downregulates proliferation genes, favoring differentiation .

Neuroprotection

PGAM1 protects against ischemic injury:

Tat-PGAM1 treatment: Reduces neuronal death, oxidative stress, and microglial activation in spinal cord ischemia models .

Cancer Metabolism

PGAM1 overexpression correlates with aggressive tumors (e.g., uveal melanoma):

Mechanisms:
- Promotes glycolysis and serine synthesis.
- Interacts with PD-L1, Bcl-2, and Snail to regulate immune evasion, apoptosis, and epithelial-to-mesenchymal transition (EMT) .
Therapeutic Target: PGAM1 inhibition may suppress tumor growth and metastasis .

Metabolic Disorders

Heterozygous Pgam1+/− mice exhibit:

Normal glucose tolerance: Despite 50% reduced PGAM1 activity .
No significant lipid or protein abnormalities: Suggesting compensatory metabolic pathways .

Table 2: PGAM1’s Role in Disease Models

Model	PGAM1 Manipulation	Outcome
Allergic Airway	T cell-specific KO	Reduced mucus production, eosinophil infiltration, and muc5ac mRNA.
EAE Autoimmunity	T cell-specific KO	Complete resistance to disease onset.
Spinal Cord Ischemia	Tat-PGAM1 treatment	Improved neurological function, reduced oxidative damage.
Uveal Melanoma	PGAM1 knockdown	Inhibited migration, induced apoptosis, and suppressed EMT.

Product Specs

Introduction

PGAM1, a member of the phosphoglycerate mutase family, plays a crucial role in glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism. It catalyzes the reversible conversion of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) within the glycolytic pathway. This dimeric enzyme exists in different isoforms depending on the tissue, including a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Mutations in PGAM1 can lead to muscle phosphoglycerate mutase deficiency, also known as glycogen storage disease X.

Description

Recombinant PGAM1 Mouse, produced in E.coli, is a single, non-glycosylated polypeptide chain consisting of 278 amino acids (1-254) with a molecular mass of 31.4kDa. This protein is fused to a 24 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.

Physical Appearance

Clear, colorless, and sterile-filtered solution.

Formulation

The PGAM1 solution is provided at a concentration of 1mg/ml in a buffer containing 20mM Tris-HCl (pH 8.0), 20% glycerol, 0.1M NaCl, and 1mM DTT.

Stability

For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.

Purity

Purity is determined to be greater than 95% by SDS-PAGE analysis.

Synonyms

Phosphoglycerate mutase 1, BPG-dependent PGAM 1, Phosphoglycerate mutase isozyme B, PGAM-B, Pgam1, Pgam-1, 2310050F24Rik.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMAAYKL VLIRHGESAW NLENRFSGWY DADLSPAGHE EAKRGGQALR DAGYEFDICF TSVQKRAIRT LWTVLDAIDQ MWLPVVRTWR LNERHYGGLT GLNKAETAAK HGEAQVKIWR RSYDVPPPPM EPDHPFYSNI SKDRRYADLT EDQLPSCESL KDTIARALPF WNEEIVPQIK EGKRVLIAAH GNSLRGIVKH LEGLSEEAIM ELNLPTGIPI VYELDKNLKP IKPMQFLGDE ETVRKAMEAV AAQGKVKK.

Product Science Overview

Structure and Function

PGAM1 belongs to the phosphoglycerate mutase family and is a dimeric enzyme. It exists in different isoforms, including a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB) found in various tissues. The enzyme is essential for efficient energy production and is involved in the metabolism of glucose and 2,3-bisphosphoglycerate (2,3-BPGA).

Recombinant Mouse PGAM1

Recombinant mouse PGAM1 is a bioactive protein that has been engineered for research purposes. It is typically expressed in E. coli and purified using conventional chromatography techniques. The recombinant version often includes a His-tag at the N-terminus to facilitate purification and detection.

Applications in Research

Recombinant PGAM1 is used in various biochemical and physiological studies to understand its role in metabolism and disease. For instance, it has been studied in the context of cancer metabolism, where it is known to promote tumor progression by inducing immunosuppressive M2 macrophages. This makes PGAM1 a potential target for cancer therapy, particularly in breast cancer.

Storage and Stability

For optimal stability, recombinant mouse PGAM1 should be stored at 4°C for short-term use and at -20°C for long-term storage. It is important to avoid freeze-thaw cycles to maintain its activity.

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Source

Quantity&Size*

Product Name

Message

PGAM1 Mouse

Description

Biochemical Characterization of PGAM1 in Mice

Genetic Models of Pgam1 in Mice

Table 1: Genetic Models of Pgam1 in Mice

Immune Function

Reproductive Biology

Neuroprotection

Cancer Metabolism

Metabolic Disorders

Table 2: PGAM1’s Role in Disease Models

Product Specs

Product Science Overview

Quick Inquiry

Category

Service

PGAM1 Mouse

Description

Biochemical Characterization of PGAM1 in Mice

Genetic Models of Pgam1 in Mice

Table 1: Genetic Models of Pgam1 in Mice

Immune Function

Reproductive Biology

Neuroprotection

Cancer Metabolism

Metabolic Disorders

Table 2: PGAM1’s Role in Disease Models

Product Specs

Product Science Overview

Quick Inquiry

Category

Service

Related Products