PMSG

Pregnant Mare Serum Gonadotropin
Shipped with Ice Packs
In Stock

Description

Physiological Roles and Mechanisms

PMSG mimics endogenous gonadotropins to stimulate ovarian follicle growth and ovulation. Key mechanisms include:

Follicular Development

PMSG induces follicular maturation by binding to FSH receptors, promoting granulosa cell proliferation and estradiol synthesis. In rodents, superovulation protocols typically involve PMSG doses (e.g., 4–40 IU) followed by human chorionic gonadotropin (hCG) administration .

Uterine Glycoconjugate Alterations

Studies in rats reveal dose-dependent effects on endometrial glycoconjugates critical for implantation:

PMSG Dose (IU)Apical MembraneGolgi ZoneBasement MembraneUterine Gland
4No changeNo changeNo changeNo change
8Minimal alterationMinimal changeSlight alterationModerate change
24Significant changeSignificantSignificantSignificant
40Moderate alterationModerateModerateSlight change

Optimal dose for minimal uterine disruption: 8 IU .

Applications in Research and Veterinary Medicine

PMSG is widely used in:

Superovulation and Embryo Production

  • Laboratory Animals: Induces ovulation in mice, rats, and livestock to enhance embryo yield .

  • Heat Stress Adaptation: Under high temperatures, PMSG improves oocyte maturation capacity despite reduced follicle numbers .

Hormonal Studies

  • Gonadotropin Receptor Interactions: Used to study FSH/LH activity and receptor cross-reactivity .

  • Glycan Analysis: Serves as a model for glycoprotein glycosylation patterns .

Recent Research Insights

  • Dose Optimization: Lower PMSG doses (e.g., 8 IU) minimize endometrial glycoconjugate disruption, improving implantation success .

  • Heat Stress Mitigation: PMSG enhances oocyte quality in high-temperature environments, though follicle counts may decline .

  • Structural Variability: Molecular weight discrepancies (49,000–68,500 Da) highlight purification method impacts .

Product Specs

Introduction

Pregnant Mare Serum Gonadotropin (PMSG) is a hormone frequently used in conjunction with progestogens to enhance ovulation prior to artificial insemination. This placental glycoprotein, derived from the serum of pregnant mares, consists of alpha and beta subunits. Secreted by endometrial cups within the mare's uterus between 40 to 130 days of gestation, PMSG can be extracted and utilized to artificially induce estrus in female animals. These specialized structures produce PMSG to stimulate the mare's ovaries and reproductive system. PMSG promotes follicular growth in the ovaries, ultimately leading to ovulation. In species other than horses, PMSG has a biological half-life of approximately 4 days, contributing to its prolonged ovarian stimulation and ovulation-inducing effects. However, using PMSG alone often results in cystic ovarian disease due to excessive ovarian stimulation and the presence of sugar molecules that hinder hormone clearance. Its extended circulatory half-life makes PMSG a preferred choice over other pituitary hormones. While PMSG primarily exhibits luteinizing hormone-like activity, it demonstrates both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) like activity in other species.

Description

Pregnant Mare Serum Gonadotropin (PMSG), a complex glycoprotein extracted from the serum of pregnant mares, has a molecular weight ranging from 43 to 63 kDa. This protein can supplement or even replace the functions of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) produced by the anterior pituitary gland in both males and females. Notably, PMSG-Intervet specifically stimulates the development of ovarian follicles in females.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The PMSG product is lyophilized without any additional ingredients.
Solubility
For reconstitution, it is advised to dissolve the lyophilized PMSG in sterile 18 megaohm-centimeter (MΩ·cm) water (H2O) to achieve a concentration of 1000 international units per milliliter (IU/ml). This solution can be further diluted into other aqueous solutions if needed.
Stability
Lyophilized PMSG, while stable at room temperature for up to 3 weeks, should ideally be stored at a temperature between 2-8 degrees Celsius.
Source
Serum of pregnant mares.

Q&A

What is a PMSG and how does it function in renewable energy systems?

A Permanent Magnet Synchronous Generator (PMSG) is an electrical machine that converts mechanical energy into electrical energy using permanent magnets to create the magnetic field required for induction. In renewable energy applications, particularly tidal and wind energy systems, PMSGs function by:

  • Converting mechanical rotation from turbines directly into electrical energy

  • Outputting AC power that is typically rectified and stabilized by PWM (Pulse Width Modulation) rectifiers

  • Providing output that can be integrated into power grids or used in standalone applications

The basic operation involves the PMSG converting mechanical energy into electrical energy, with the output AC power being rectified and stabilized before integration into the power system. Unlike induction generators, PMSGs do not require external excitation circuits, making them more efficient for certain renewable energy applications .

What are the fundamental parameters that affect PMSG performance in experimental setups?

When designing experimental setups for PMSG research, several key parameters must be carefully considered and controlled:

ParameterTypical ValueSignificance in Research
Inverse electromotive force190 VAffects power output capacity
Equivalent inductance0.3 mHInfluences dynamic response
Equivalent resistance0.1 ΩDetermines power losses
External inductors1.7 mHControls harmonic content
DC-side capacitance1600 μFStabilizes output voltage
Sampling frequency10 kHzCritical for control accuracy

These parameters significantly affect experimental outcomes and must be properly characterized before conducting advanced research. Researchers should establish baseline performance under controlled conditions by measuring output characteristics at various mechanical input speeds .

How do PMSGs compare with Self-Excited Induction Generators (SEIGs) for small-scale renewable energy research?

When comparing PMSGs with SEIGs for research purposes, several methodological considerations emerge:

  • PMSGs typically demonstrate higher efficiency at partial loads compared to SEIGs, which is crucial for variable renewable resources

  • SEIGs require capacitor banks for self-excitation, introducing additional variables in experimental design

  • PMSGs offer better voltage regulation characteristics under varying load conditions

  • Transient response characteristics differ significantly between the two technologies, requiring different measurement protocols

Experimental investigations have shown that while SEIGs may be more robust and cost-effective, PMSGs generally provide superior performance in terms of efficiency and power quality for small-scale renewable applications. Research methodologies must account for these fundamental differences when designing comparative studies .

What control strategies demonstrate optimal performance for PMSG-PWM systems under variable operating conditions?

For PMSG systems operating under variable conditions (such as fluctuating tidal or wind resources), advanced control strategies are essential. Research indicates that dual-loop control architectures provide superior performance:

  • Voltage Outer Loop Control: The ESO-based (Extended State Observer) variable exponential convergence law SMC (Sliding Mode Control) methodology has demonstrated exceptional performance in experimental studies. This approach effectively:

    • Responds quickly across wide output ranges

    • Minimizes DC output voltage overshoot

    • Reduces voltage jitter significantly

    • Enhances system anti-interference capabilities

  • Current Inner Loop Control: The MDPDC (Modified Direct Power and Direct Current) scheme with time-delay compensation improves:

    • Current tracking speed and accuracy

    • Maintenance of high power factor operation during external disturbances

    • Significant reduction in harmonic content

Researchers should implement these control strategies using real-time control platforms with sampling frequencies of at least 10 kHz to achieve optimal results. The parameters for the sliding mode controller should be carefully tuned based on system characteristics .

How can researchers effectively address harmonics and voltage fluctuations in PMSG experimental studies?

Harmonics and voltage fluctuations represent significant challenges in PMSG research. Methodological approaches to address these issues include:

  • Implementation of advanced PWM rectification technologies that incorporate:

    • Sliding mode observers for enhanced state estimation

    • Time-delay compensation mechanisms

    • Harmonic suppression algorithms

  • Experimental verification should follow a structured approach:

    • Begin with rated condition testing

    • Progress to variable operating conditions

    • Test under DC-side voltage variation scenarios

    • Implement spectral analysis to quantify harmonic content under each condition

Research has demonstrated that the current inner loop adopting MDPDC schemes with time-delay compensation significantly improves current tracking while maintaining high power factor operation. The harmonic suppression effect is particularly significant compared to conventional control methods, reducing potential damage from harmonics .

What experimental methodologies are recommended for validating PMSG control systems?

Rigorous experimental validation of PMSG control systems requires a comprehensive methodology:

  • Structured Testing Sequence:

    • First, conduct tests under rated conditions to establish baseline performance

    • Next, implement variable operating conditions to simulate real-world fluctuations

    • Finally, introduce deliberate DC-side voltage variations to test controller robustness

  • Performance Metrics Measurement:

    • Voltage response characteristics (overshoot, settling time, steady-state error)

    • Current tracking accuracy and harmonic content

    • Power factor under various loading conditions

    • System efficiency across operating range

  • Data Acquisition and Analysis:

    • Use high-speed sampling (minimum 10 kHz) to capture transient behaviors

    • Apply spectral analysis to quantify harmonic content

    • Implement statistical methods to evaluate control consistency

The experimental results should demonstrate that the control system maintains high accuracy and fast response even when operating conditions change significantly, which is essential for renewable energy applications where resource availability fluctuates naturally .

What are the integration challenges when incorporating PMSGs into different power grid architectures?

PMSG integration with power grids presents several research challenges that require methodological solutions:

  • Grid Compatibility Issues:

    • Voltage level matching between PMSG output and grid requirements

    • Frequency synchronization methodologies

    • Power quality compliance verification techniques

  • Research Approaches for Grid Integration:

    • Development of adaptive control strategies that can accommodate different grid specifications

    • Implementation of power electronic interfaces with variable DC output voltage capabilities

    • Design of fault ride-through protocols specific to PMSG characteristics

Recent research demonstrates that PMSG-PWM systems implementing ESO-based variable exponential convergence law SMC can maintain high control accuracy and response speed even when DC output voltage changes, facilitating integration with different levels of power grids. This adaptability is crucial for research applications where PMSGs may need to interface with various experimental grid configurations .

How should researchers approach the comparative analysis of various PMSG topologies for specific renewable applications?

Comparative analysis of PMSG topologies requires a structured experimental framework:

  • Standardized Testing Protocol:

    • Define consistent performance metrics across all topologies

    • Establish identical test conditions (mechanical input, electrical load, ambient conditions)

    • Use calibrated instrumentation to ensure measurement accuracy

  • Performance Evaluation Criteria:

    • Efficiency mappings across operating ranges

    • Dynamic response characteristics

    • Harmonics generation profiles

    • Robustness to external disturbances

  • Application-Specific Testing:

    • For tidal applications: cyclic loading patterns that mimic tidal flows

    • For wind applications: stochastic input variations that reflect wind patterns

    • For hybrid systems: interaction effects between multiple energy sources

Research indicates that improved topologies for PWM rectification of low load-to-wave ratio PMSGs can significantly enhance system performance. Comparative studies should particularly focus on the behavior under partial loading conditions, which frequently occur in renewable energy applications .

What data acquisition approaches are recommended for PMSG experimental studies?

Robust data acquisition is fundamental to meaningful PMSG research:

  • Instrumentation Requirements:

    • High-resolution voltage and current sensors with adequate bandwidth

    • Torque and speed measurement devices for mechanical input characterization

    • Temperature monitoring for thermal performance evaluation

    • Power analyzers capable of harmonic analysis

  • Sampling Considerations:

    • Minimum sampling frequency of 10 kHz for control variables

    • Higher sampling rates (50+ kHz) for harmonic analysis

    • Synchronized multi-channel acquisition for phase relationship studies

  • Data Processing Methodologies:

    • Real-time filtering techniques to minimize measurement noise

    • Spectral analysis methods for harmonic quantification

    • Statistical processing for uncertainty quantification

The experimental verification presented in the literature demonstrates the importance of comprehensive data acquisition approaches that capture both steady-state and transient behaviors across various operating conditions .

How can researchers effectively troubleshoot inconsistent experimental results in PMSG studies?

When encountering experimental inconsistencies, researchers should follow a systematic troubleshooting methodology:

  • Parameter Verification:

    • Reconfirm all system parameters against design values (see parameter table below)

    • Verify calibration of all measurement instruments

    • Check for environmental factors affecting results (temperature, electromagnetic interference)

ParameterDesign ValueAcceptable Tolerance
Inverse electromotive force190 V±5%
Equivalent inductance0.3 mH±10%
External inductors1.7 mH±10%
DC-side capacitance1600 μF±5%
Switching frequency10 kHz±0.1%
  • Systematic Isolation Testing:

    • Decouple control loops to identify specific problematic components

    • Implement step-by-step testing from basic to complex functionality

    • Compare results with theoretical models to identify divergence points

  • Control Parameter Adjustment:

    • Fine-tune controller parameters (switching function coefficients, exponential convergence coefficients)

    • Implement alternative control strategies to compare performance

    • Analyze sensitivity to parameter variations

Research indicates that many inconsistencies in PMSG experimental results stem from improper parameter settings or insufficient consideration of external disturbances. The implementation of observers (such as ESO) can significantly improve experimental consistency by compensating for unmeasured disturbances .

Product Science Overview

Origin and Production

PMSG is secreted by the endometrial cups formed in the uterus of pregnant mares approximately 40 days after conception . These endometrial cups are specialized structures that develop from the fetal trophoblast cells and invade the maternal endometrium. The secretion of PMSG peaks around 60 days of gestation and can be detected in the mare’s serum until about 100-150 days post-conception .

Structure and Function

PMSG is a glycoprotein hormone composed of two subunits: alpha and beta. The alpha subunit is common to all glycoprotein hormones, including luteinizing hormone (LH), follicle-stimulating hormone (FSH), and thyroid-stimulating hormone (TSH). The beta subunit is unique to PMSG and is responsible for its specific biological activity .

In equids, PMSG exhibits primarily LH-like activity, which is essential for maintaining the corpus luteum and supporting early pregnancy. In other species, such as sheep, goats, cattle, and swine, PMSG has both FSH and LH-like activities, making it useful for inducing ovulation and synchronizing estrus cycles .

Applications in Veterinary Medicine

PMSG is widely used in veterinary medicine and animal husbandry due to its ability to induce ovulation and synchronize estrus in various livestock species. It is commonly administered in combination with progestogens to control the timing of ovulation and improve reproductive efficiency in breeding programs .

For example, in sheep and goats, PMSG is used to induce estrus and ovulation, allowing for timed artificial insemination. In cattle, it is used to synchronize estrus cycles, facilitating the management of breeding schedules. In swine, PMSG is used to induce estrus in gilts and sows, improving the efficiency of breeding operations .

Ethical Considerations

The production of PMSG has raised ethical concerns due to the methods used to collect the hormone from pregnant mares. Reports have highlighted inhumane practices in some horse farms, particularly in countries like Uruguay, Argentina, and Iceland. These practices involve the collection of large volumes of blood from pregnant mares, leading to potential health and welfare issues for the animals .

Efforts are being made to address these ethical concerns and improve the welfare of mares used in PMSG production. This includes the development of alternative methods for hormone collection and the implementation of stricter regulations to ensure humane treatment of animals .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
© Copyright 2024 Thebiotek. All Rights Reserved.