POLR2I Human
Description
Functional Roles in RNA Polymerase II
POLR2I (also termed RPB9) is a core component of Pol II and contributes to:
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DNA Binding: Forms part of the upper jaw of Pol II’s central cleft, stabilizing the DNA template during transcription .
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Transcription Fidelity: Zinc finger domains enhance transcription accuracy by proofreading RNA-DNA mismatches .
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Interaction Partners: Cooperates with subunits POLR2C, POLR2D, POLR2J, and POLR2L to maintain polymerase structural integrity .
Hypertensive Nephropathy (HN)
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Upregulation: POLR2I expression is elevated in HN patients and correlates with renal dysfunction (r = 0.95, P = 1×10⁻³⁹) .
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Mechanistic Insights:
Cancer
| Cancer Type | POLR2I Dysregulation | Clinical Association |
|---|---|---|
| Ovarian Cancer | Upregulated | Tumor progression . |
| Head and Neck Cancer | Upregulated | Poor prognosis . |
| Hepatocellular Carcinoma | Downregulated | Reduced survival . |
Recombinant POLR2I in Research
Recombinant POLR2I protein (e.g., ab81884, ENZ-669) is widely used for:
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Biochemical Assays: Studying Pol II assembly and transcription mechanisms .
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Antibody Production: Generating antibodies for immunofluorescence and Western blotting .
Clinical and Therapeutic Implications
Product Specs
Product Science Overview
POLR2I is a member of the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family . The human recombinant form of POLR2I is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain containing 148 amino acids. It has a molecular mass of approximately 17.0 kDa . The recombinant protein is often fused to a 23 amino acid His-tag at the N-terminus to facilitate purification through chromatographic techniques .
POLR2I plays a critical role in the transcription process by contributing to the formation of the RNA polymerase II complex. This complex catalyzes the transcription of DNA into RNA using ribonucleoside triphosphates as substrates . Specifically, POLR2I is involved in synthesizing mRNA precursors and various functional non-coding RNAs, which are essential for the regulation of gene expression .
The recombinant POLR2I protein is produced in E. coli and is purified using proprietary chromatographic techniques to ensure high purity and activity . The protein solution is typically formulated with 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 20% glycerol, and 1mM DTT to maintain stability . For long-term storage, it is recommended to add a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) and to avoid multiple freeze-thaw cycles .
POLR2I is widely used in laboratory research to study the mechanisms of transcription and gene regulation. It is also utilized in various biochemical assays to investigate the interactions between RNA polymerase II subunits and other transcription factors . The recombinant form of POLR2I allows researchers to conduct experiments with a consistent and reliable source of the protein, facilitating advancements in our understanding of transcriptional regulation.
