RAB27B Human

RAB27B, Member RAS Oncogene Family Human Recombinant
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Cat. No.
BT8527
Source
Escherichia Coli.
Synonyms

Ras-related protein Rab-27B, C25KG, RAB27B.

Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Exocytosis and Vesicle Trafficking

RAB27B facilitates the delivery of secretory granules to the plasma membrane in specialized cells:

  • Platelet Dense Granules: Regulates granule formation, secretion, and serotonin content. Rab27b knockout mice exhibit reduced platelet dense granule numbers and impaired aggregation .

  • Exosome Secretion: Mediates vesicle transfer from the trans-Golgi network to multivesicular bodies (MVBs), a precursor to exosome biogenesis .

  • Lysosomal Function: Promotes acidic vesicle trafficking and lysosomal protease activity (e.g., cathepsin D), critical for α-synuclein clearance in neurons .

Disease-Related Pathways

DiseaseMechanism
Renal Cell CarcinomaOverexpression correlates with tumor progression and sunitinib resistance .
NeurodegenerationDefects in lysosomal proteolysis exacerbate α-synuclein aggregation .
Platelet DysfunctionReduced dense granule secretion causes bleeding diathesis .

Molecular Interactions and Effectors

RAB27B binds effector proteins to execute its functions:

EffectorFunction
Melanophilin/MLPHLinks Rab27 proteins to myosin motors for vesicle transport .
MyRIPAnchors PKA to vesicles, facilitating exocytosis .
Synaptotagmin-like (SYTL)Binds phospholipids, regulating cytotoxic granule exocytosis .
Rab27aPartial functional redundancy in dense granule secretion .

Clinical and Research Implications

Prognostic Biomarker

  • Cancer: High RAB27B expression correlates with poor survival in renal cell carcinoma (RCC) and pancreatic adenocarcinoma .

  • Neurodegeneration: Elevated RAB27B in incidental Lewy Body Disease (iLBD) suggests a role in α-synuclein pathology .

Therapeutic Targeting

  • Knockdown Studies: Silencing RAB27B inhibits cancer cell proliferation and invasion .

  • Overexpression: Enhances lysosomal activity and reduces α-synuclein aggregates in neurons .

Research Applications

ApplicationMethod
Protein StudiesRecombinant RAB27B (1–218 aa, E. coli) for SDS-PAGE, mass spectrometry .
Knockout ModelsRab27b−/− mice show impaired platelet function and lysosomal defects .
Exosome AnalysissiRNA-mediated knockdown to study vesicle secretion .

Product Specs

Introduction
RAB27B, a member of the RAB family, plays a crucial role in regulating vesicular transport. While found in the pituitary gland, RAB27B exhibits higher expression levels in the brain and spleen. Notably, it might share functional redundancy with Rab27a, as evidenced by its ability to compensate for Rab27a mutations.
Description
RAB27B Human Recombinant, with a 20 amino acid His tag at the N-terminus, is produced in E. coli. This single, non-glycosylated polypeptide chain comprises 238 amino acids (1-218 a.a.) and has a molecular weight of 26.7 kDa. The purification process for RAB27B involves proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered, colorless solution.
Formulation
The RAB27B solution (1 mg/ml) is supplied in a buffer containing 20mM Tris-HCl (pH 8.0), 0.1M NaCl, 1mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeding 90.0% as determined by SDS-PAGE analysis.
Synonyms

Ras-related protein Rab-27B, C25KG, RAB27B.

Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MTDGDYDYLI KLLALGDSGV GKTTFLYRYT DNKFNPKFIT TVGIDFREKR VVYNAQGPNG SSGKAFKVHL QLWDTAGQER FRSLTTAFFR DAMGFLLMFD LTSQQSFLNV RNWMSQLQAN AYCENPDIVL IGNKADLPDQ REVNERQARE LADKYGIPYF ETSAATGQNV EKAVETLLDL IMKRMEQCVE KTQIPDTVNG GNSGNLDGEK PPEKKCIC.

Product Science Overview

Gene and Protein Information
  • Gene Symbol: RAB27B
  • Protein Name: Ras-related protein Rab-27B
  • Aliases: C25KG, EC 3.6.5.2
  • HGNC ID: 9767
  • NCBI Gene ID: 5874
  • Ensembl ID: ENSG00000041353
  • UniProtKB/Swiss-Prot ID: O00194
Function and Mechanism

RAB27B plays a crucial role in the multivesicular body sorting pathway and positively regulates exocytosis . In its active state, RAB27B binds to various effector proteins to maintain homeostasis in the late endocytic pathway . It is also involved in the axonal anterograde transport of NTRK2/TRKB by facilitating its association with KLC1 .

Expression and Localization

RAB27B is expressed in several tissues, including the brain, spleen, and pituitary tissue . It is located in the Golgi stack and cytoplasmic vesicles . The protein is also involved in targeting uroplakins to urothelial apical membranes .

Associated Diseases

Mutations or dysregulation of RAB27B have been associated with several diseases, including:

  • Martin-Probst Syndrome
  • Griscelli Syndrome, Type 1
Pathways

RAB27B is involved in several biological pathways, such as:

  • Response to elevated platelet cytosolic Ca2+
  • Vesicle-mediated transport
Research and Clinical Relevance

Due to its role in vesicular transport and disease association, RAB27B is a significant target for research in understanding cellular transport mechanisms and developing therapeutic interventions for related diseases.

For more detailed information, you can refer to the GeneCards entry on RAB27B and the Human Protein Atlas.

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