Recombinant Human Brain-derived neurotrophic factor (BDNF) (Active)
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Description
Molecular Structure and Production
Recombinant Human BDNF is a 119-amino-acid protein dimer stabilized by hydrophobic interactions, with a molecular weight of 27 kDa . It is expressed in Escherichia coli or baculovirus systems, achieving >95% purity via SDS-PAGE . Key structural features include:
Mechanism of Action
BDNF activates TrkB receptors and the low-affinity p75NTR, triggering downstream pathways:
Proteolytic cleavage of pro-BDNF (precursor) determines functional outcomes: mature BDNF promotes survival, while pro-BDNF may induce apoptosis .
Neuroprotection and Synaptic Plasticity
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Rescues motor neurons after sciatic nerve injury in vitro (EC₅₀ = 220 pM) .
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Enhances dendritic complexity in cortical neurons by 40–60% under activity-dependent conditions .
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Restores hippocampal long-term potentiation (LTP) in BDNF-knockout mice .
Clinical Trials in Neurodegenerative Diseases
A phase II trial in 1,135 ALS patients tested subcutaneous BDNF (25 μg/kg vs. 100 μg/kg):
| Parameter | 25 μg/kg | 100 μg/kg | Placebo |
|---|---|---|---|
| 9-month survival rate | 85% | 89% | 85% |
| Forced Vital Capacity (≤91%) | No benefit | 22% improvement | – |
| Adverse effects (bowel) | 12% | 20% | 8% |
Post hoc analysis showed survival benefits in patients with early respiratory impairment .
Applications and Therapeutic Potential
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Neurodegenerative Diseases: Delays motor neuron loss in ALS models and reduces β-amyloid toxicity in Alzheimer’s disease .
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Cardiovascular Protection: Activates endothelial nitric oxide synthase (eNOS) via TrkB, improving vascular function .
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Psychiatric Disorders: Low BDNF correlates with depression and schizophrenia; supplementation reverses synaptic deficits in rodent models .
Product Specs
Buffer
Lyophilized from a 0.2 µm filtered 20 mM phosphate buffer (PB), 250 mM sodium chloride (NaCl), pH 7.2
Form
Lyophilized powder
Lead Time
Typically, we can ship your orders within 5-10 business days after receiving them. Delivery timelines may vary depending on the order method and location. For specific delivery times, please consult your local distributors.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Reconstitution
We recommend centrifuging the vial briefly before opening to ensure the contents settle at the bottom. Reconstitute the protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting the solution at -20°C/-80°C. Our default final concentration of glycerol is 50%, which can be used as a reference.
Shelf Life
The shelf life of the product is influenced by several factors, including storage conditions, buffer composition, temperature, and the intrinsic stability of the protein. Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquot the product for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag-Free
Synonyms
Abrineurin; ANON2; BDNF; BDNF_HUMAN; Brain Derived Neurotrophic Factor; Brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin
Datasheet & Coa
Please contact us to get it.
Expression Region
19-247aa
Mol. Weight
25.9 kDa
Protein Length
Full Length of Mature Protein
Purity
Greater than 95% as determined by SDS-PAGE.
Research Area
Neuroscience
Source
E.coli
Species
Homo sapiens (Human)
Target Names
Uniprot No.
Target Background
Function
Brain-derived neurotrophic factor (BDNF) is a crucial signaling molecule that activates downstream signaling cascades initiated by NTRK2. During development, it plays a vital role in promoting the survival and differentiation of specific neuronal populations within the peripheral and central nervous systems. BDNF participates in axonal growth, pathfinding, and the modulation of dendritic growth and morphology. It serves as a major regulator of synaptic transmission and plasticity at adult synapses in various regions of the central nervous system (CNS). The versatility of BDNF is evident in its contribution to a range of adaptive neuronal responses, including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, and homeostatic regulation of intrinsic neuronal excitability.
BDNF is also a significant signaling molecule that activates signaling cascades downstream of NTRK2. It activates these cascades through the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 is involved in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. It also promotes neuronal growth cone collapse.
BDNF is also a significant signaling molecule that activates signaling cascades downstream of NTRK2. It activates these cascades through the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 is involved in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. It also promotes neuronal growth cone collapse.
Gene References Into Functions
- In a study involving 181 suicide cases (47 early suicide cases and 134 late suicide cases) and 162 controls, individuals with the BDNF Met allele exhibited a 1.42-fold increased risk of suicide compared to those with the Val allele. When suicide cases were stratified by occurrence time, patients with the Met/Val or Met/Met genotype displayed a 2.48-fold increased risk of early suicide compared to those with the Val/Val genotype. PMID: 29734216
- Low brain-derived neurotrophic factor levels may contribute to the pathogenesis of schizophrenia, but may not be directly linked to its cognitive impairments. PMID: 29482040
- A longitudinal population-based study of older adults (n = 2,218) investigated the impact of physical activity on cognitive preservation across various domains, including processing speed, attention, working memory, and episodic verbal memory. The study utilized growth curve modeling to assess whether the benefits of physical activity differed based on BDNF genotype and sex. PMID: 29402782
- The Mn-SOD V allele has been associated with a worse outcome profile after stroke, potentially due to increased nitrosative stress, inflammatory and apoptotic responses, and a corresponding reduction in BDNF. PMID: 30150066
- Individuals with the BDNF Val66Met Megroup displayed significant reduction in short-interval intracortical inhibition (SICI) in the bilateral M1 in response to motor training. However, SICI remained unchanged in the BDNF Val66Met group. PMID: 29856758
- Research indicates a correlation between the BDNF variant rs6265 and emotional symptoms in the early phase after mild traumatic brain injury. PMID: 29357818
- A study observed that the Met66 allele of the Val66Met polymorphism in the BDNF gene was associated with decreased serum BDNF levels in individuals with self-reported risk-taking propensity among heroin users. PMID: 30134233
- Serum BDNF levels and the BDNF Val66Met polymorphism in healthy young adults exhibited a relationship with sleep patterns on weekends but not on weekdays, suggesting a possible connection between the systems involved in BDNF control and endogenous sleep characteristics rather than the socially constrained sleep schedule in healthy young adults. PMID: 29944703
- A study investigated the role of neurotrophic factors and hippocampal activity in post-traumatic stress disorder (PTSD). PMID: 29799860
- Upregulation of PAI-1 may be a crucial mechanism underlying insufficient neurotrophic support and increased neurodegeneration associated with Alzheimer's disease (AD). Targeting BDNF maturation through pharmacological inhibition of PAI-1 might offer a potential therapeutic strategy for AD. PMID: 28132883
- Research suggests that the genetic correlates of early trauma in schizophrenia patients may be linked to the BDNF Met allele carriers. PMID: 28711474
- A study revealed significant differences in blood BDNF levels between individuals with epilepsy and healthy subjects. PMID: 30140987
- Research indicates that brain-derived neurotrophic factor concentrations in serum and peritoneal fluid were significantly higher in women with endometriosis experiencing pain compared to women with endometriosis without pain. PMID: 28954602
- The Met allele of the Val66Met polymorphism in the BDNF gene is associated with lower BMI-SDS (body mass index standard deviation score) in children. PMID: 28960774
- Platelet BDNF and SERT (serotonin transporter) levels do not appear to be directly linked to psychosocial deficits in stage Huntington's Disease. However, higher BDNF storage may be associated with delayed mild symptoms. PMID: 30039833
- Direct within-family analysis revealed that fathers with the Met allele were more likely than Val/Val carriers to exhibit differential parenting towards twins who differed in their prosocial behavior. A similar pattern of findings was observed with mother-rated and experimentally assessed prosociality. PMID: 28523227
- The results of a study suggest that the Val66Met polymorphism does not predict long-term functional mobility following a stroke. PMID: 29480080
- A systematic review and meta-analysis indicate that polymorphisms in the BDNF gene, including rs925946, rs10501087, rs6265, and rs988712, may be considered as genetic determinants of obesity. PMID: 28818748
- A single nucleotide polymorphism found in the BDNF-AS (BDNF antisense RNA [nonprotein coding]) gene may be related to the decreased plasma brain-derived neurotrophic factor levels observed in frail elderly individuals. PMID: 27449141
- The pattern of low BDNF and high inflammation in major depressive disorder (MDD) may be influenced by the Val66Met polymorphism. The association of this polymorphism with inflammatory markers, in addition to BDNF levels, suggests an interplay between these systems. PMID: 28656803
- Angiogenin plays a role in angiogenesis induced by Brain-derived neurotrophic factor (BDNF). PMID: 29573867
- Findings suggest that the polymorphism Val66Met is not associated with temporal lobe epilepsy caused by hippocampal sclerosis, epilepsy-related factors, and psychiatric comorbidities in a specific group of patients. PMID: 30015148
- Research indicates that the BDNF 196 G>A polymorphism may serve as a genetic marker for predicting insulin resistance before initiating risperidone treatment in autism spectrum disorder patients. PMID: 29369497
- No significant difference was found in the BDNF Val66Met polymorphism between patients with generalized anxiety disorder (GAD) and healthy controls. Furthermore, this polymorphism was not significantly associated with antidepressant drug efficacy for GAD. PMID: 29446659
- Single nucleotide polymorphisms in the BDNF gene are involved in dopaminergic metabolism and motor and cognitive function in older adults. PMID: 29525179
- BDNF rs11030101 and BDNF rs61888800 are associated with changes in temperament scores in a clinical sample of individuals with major depression (MDD) who received selective serotonin reuptake inhibitor treatment. PMID: 29310728
- A study found that harsh parenting predicted an increased error-related negativity only among children with a methionine allele of the BDNF genotype. The study also provided evidence of moderated mediation, indicating that the ERN (error-related negativity) mediated the relationship between parenting and internalizing diagnoses and dimensional symptoms only in children with a methionine allele. PMID: 28427482
- Findings suggest a strong influence of seasonality on depression outcome and BDNF expression in atopic dermatitis and psoriasis. PMID: 27409526
- Serum BDNF levels were significantly lower in patients with Parkinson's disease compared to patients with essential tremor and controls. PMID: 29350074
- A systematic review aimed to provide a comprehensive analysis of clinical trials investigating the effect of interval aerobic training (IAT) and continuous aerobic training (CAT) on peripheral brain-derived neurotrophic factor (BDNF) concentration in older adults. PMID: 28498065
- Research suggests that BDNF modulates granulosa cell functions, potentially through the FSHR-coupled signaling pathway, to influence aromatase-mediated steroidogenesis. PMID: 28282971
- The functional Val66Met BDNF polymorphism is not associated with BDNF serum levels in acute episodes of schizophrenia and depression. PMID: 29331787
- A study demonstrated a significantly higher incidence of defective BDNF expression in the granule layers of the cerebellar cortex. PMID: 29174061
- Combining cognitive and physical exercise did not show an additional or synergistic effect on peripheral BDNF levels compared to physical exercise alone. PMID: 29842831
- A review/meta-analysis suggests a lower risk of ischemic stroke for the GG genotype of BDNF rs6265. PMID: 29449128
- Research indicated an interaction between the BDNF SNP rs1157659 and mild traumatic brain injury in predicting hippocampal volume. PMID: 28755387
- A study found that while there was no significant difference in peripheral BDNF levels between ADHD patients and control groups overall, BDNF levels were significantly higher in males with ADHD compared to controls. PMID: 29132072
- Research provided evidence supporting the hypothesis that the BDNF Val66Met polymorphism moderates the relationship between stress and depression. PMID: 29102837
- The BDNF Val66Met polymorphism is associated with functional tuning of behaviorally-relevant frontolimbic circuitry, particularly involving the ventromedial prefrontal cortex, during higher-order learning. PMID: 28867340
- MicroRNA-103 suppresses glioma cell proliferation and invasion by targeting BDNF. PMID: 29257320
- High expression levels of BDNF have been observed in cervical cancer. BDNF plays a role in epithelial-mesenchymal transition, migration, and invasion of cervical cancer cells. PMID: 29345295
- A study observed a statistically significant change in BDNF levels post-chemotherapy in early-stage breast cancer patients. Furthermore, plasma BDNF levels were associated with self-perceived concentration deficits in patients receiving chemotherapy. PMID: 29258453
- A meta-analysis/review suggests that patients with ischemic stroke at high risk of post-stroke depression have lower BDNF levels in the early stages of stroke. PMID: 29128330
- Patients with coronary artery disease exhibited significantly lower plasma BDNF and higher vWF (von Willebrand factor) levels compared to control patients. PMID: 29409455
- Brain-derived neurotrophic factor (BDNF) has been identified as the downstream target of miR-107 in breast cancer. PMID: 27813254
- Research demonstrated that first-episode psychosis subjects exhibit global DNA hypo-methylation and reduced BDNF gene expression. PMID: 29056292
- BDNF is down-regulated in cord blood by prenatal smoking. PMID: 28130959
- The BDNF/TrkB axis plays a role in epithelial mesenchymal transition, promoting the acquisition of (myo)fibroblast cell phenotype in idiopathic pulmonary fibrosis. PMID: 28938915
- Results indicate that Lnc RNA BDNF-AS inversely regulates the expression level of BDNF, which modulates high-glucose induced apoptosis in human retinal pigment epithelial cells. PMID: 28657668
- A study revealed a linear relationship between the BDNF Val66Met genotypes and plasma BDNF levels in Caucasian depressed patients. PMID: 28848102
Database Links
Involvement In Disease
Bulimia nervosa 2 (BULN2); Congenital central hypoventilation syndrome (CCHS)
Protein Families
NGF-beta family
Subcellular Location
Secreted.; [BDNF precursor form]: Secreted.
Tissue Specificity
Detected in blood plasma and in saliva (at protein level). Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
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