Recombinant Human Fibroblast growth factor 2 (FGF2), partial (Active)
Description
Biological Activity and Mechanisms
FGF2, partial (Active) binds fibroblast growth factor receptors (FGFR1–4) and integrins (ITGAV:ITGB3), activating downstream pathways like RAS/MAPK . Functional assays demonstrate:
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Mitogenic Activity: Induces 3T3 fibroblast proliferation with ED50 <5 ng/ml .
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Angiogenesis: Promotes endothelial cell migration and blood vessel formation .
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Collagen Modulation: Suppresses collagen type I/III mRNA and protein expression in smooth muscle cells .
Periodontal Regeneration
A meta-analysis of four RCTs showed 0.3% rhFGF-2 significantly improved bone fill (BF%) in periodontal defects compared to controls :
| Parameter | Intervention Group | Control Group | Effect Size (95% CI) |
|---|---|---|---|
| BF% | 22.37% higher | Baseline | MD = 22.37 (13.47–31.27) |
| Linear Bone Gain | 1.13 mm | Baseline | MD = 1.13 (0.78–1.79) |
Higher doses (0.4%) showed non-significant trends, suggesting optimal efficacy at 0.3% .
Wound Healing
FGF2-impregnated collagen/gelatin sponges (CGS) demonstrated:
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Chronic Ulcers: 94% (16/17) of patients showed improved healing .
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Surgical Defects: Reduced need for autologous grafts in reconstructive surgery .
Stabilization Challenges and Solutions
FGF2’s instability in aqueous solutions limits clinical utility. Strategies include:
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Sustained-Release Systems: CGS matrices enable gradual FGF2 release over 10 days .
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Lyophilization: Maintains activity post-reconstitution at 0.1 mg/ml in sterile buffers .
Production and Quality Control
High-yield expression (up to 185.66 mg/kg in rice grains) and simplified purification protocols achieve 4.49% recovery . Functional equivalence to commercial FGF2 is validated via NIH/3T3 proliferation assays .
Product Specs
Note: All our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance. Additional fees will apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The lyophilized form has a shelf life of 12 months at -20°C/-80°C.
