Recombinant Human Insulin-like growth factor I (IGF1), partial (Active)
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Description
Molecular Structure and Production
Recombinant Human IGF1, partial (Active) corresponds to the Gly49-Ala118 fragment of the mature IGF1 protein (70 amino acids, 7.6 kDa) and retains three intramolecular disulfide bonds essential for structural stability and receptor binding . Produced in E. coli, it achieves >98% purity with low endotoxin levels (<1 EU/μg) .
| Parameter | Specification |
|---|---|
| Amino Acid Range | Gly49-Ala118 |
| Expression System | Escherichia coli |
| Purity | >98% (SDS-PAGE) |
| Activity (ED₅₀) | 0.3–1.5 ng/mL (MCF-7 cell proliferation) |
Biological Activity and Mechanisms
This recombinant fragment binds IGF1R with high affinity, activating downstream pathways such as PI3K-AKT and Ras-MAPK, which regulate cell survival, glucose uptake, and growth . Key functions include:
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Mitogenic Activity: Stimulates proliferation in breast cancer (MCF-7) and osteoblast cell lines at picomolar concentrations .
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Metabolic Regulation: Enhances glucose transport in osteoblasts at 10–100x lower concentrations than insulin .
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Cross-Species Reactivity: 94% sequence identity with murine IGF1 enables interspecies applications .
Pharmacokinetic Profile
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Dose-Dependent Kinetics: Nonlinear elimination observed at higher doses (80 μg/kg), attributed to saturation of IGF-binding proteins (IGFBPs) .
| Dose (μg/kg) | Cₘₐₓ (μg/L) | AUC (ng·h/mL) |
|---|---|---|
| 40 | 341 | 2,050 |
| 80 | 794 | 3,590 |
Clinical Applications
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Growth Disorders: In a 12-month trial, 80 μg/kg/day increased height velocity from 3.8 cm/yr to 6.6 cm/yr in IGF1-deficient patients .
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Rett Syndrome: Improved apnea frequency and anxiety scores in a phase 1 trial (40–120 μg/kg BID) .
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Metabolic Dysregulation: Reduces insulin resistance by suppressing GH secretion and normalizing IGFBP levels .
Key Studies
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IGF1 Deficiency Therapy: Subcutaneous administration (40–80 μg/kg) normalized circulating IGFBP-3 (+55%) and reduced GH peaks by 88%, correlating with lean mass gains .
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Neurological Effects: In Rett syndrome, IGF1 crossed the blood-brain barrier (CSF levels: +32%) and reversed EEG markers of anxiety .
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Cancer Research: Promoted tumor progression in vitro via AKT pathway activation, underscoring its dual role in therapy and oncogenesis .
Product Specs
Buffer
Lyophilized from a 0.2 μm filtered 20 mM sodium acetate-acetic acid buffer, pH 4.5.
Form
Lyophilized powder
Lead Time
Product shipment typically occurs within 5-10 business days of order receipt. Delivery times may vary depending on shipping method and destination. Please contact your local distributor for precise delivery estimates.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and storing in aliquots at -20°C or -80°C. Our standard protocol utilizes 50% glycerol. This concentration can be adjusted as needed.
Shelf Life
Shelf life depends on several factors including storage conditions, buffer composition, temperature, and the inherent stability of the protein. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag-Free
Synonyms
IBP1; IGF I; IGF IA; IGF IB; IGF-I; Igf1; IGF1_HUMAN; IGF1A; IGFI; IGFIA; Insulin like growth factor 1 (somatomedin C) ; Insulin like growth factor 1; Insulin like growth factor IA; Insulin like growth factor IB; Insulin-like growth factor I; Mechano growth factor; MGF; OTTHUMP00000195080; OTTHUMP00000195081; OTTHUMP00000195082; OTTHUMP00000195083; OTTHUMP00000195084; Somatomedia C; Somatomedin C; Somatomedin-C
Datasheet & Coa
Please contact us to get it.
Expression Region
52-118aa
Mol. Weight
7.3 kDa
Protein Length
Partial
Purity
Greater than 95% as determined by SDS-PAGE.
Research Area
Signal Transduction
Source
E.coli
Species
Homo sapiens (Human)
Target Names
Uniprot No.
Target Background
Function
Insulin-like growth factor 1 (IGF-1), isolated from plasma, exhibits structural and functional similarities to insulin but possesses significantly higher growth-promoting activity. It may serve as a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. IGF-1 stimulates glucose transport in bone-derived osteoblastic (PyMS) cells at concentrations considerably lower than insulin, impacting not only glycogen and DNA synthesis but also enhancing glucose uptake. It may also play a role in synapse maturation. Calcium-dependent exocytosis of IGF-1 is crucial for olfactory sensory perception. IGF-1 acts as a ligand for the IGF-1 receptor (IGF-1R), binding to its alpha subunit and activating its intrinsic tyrosine kinase activity. This autophosphorylates tyrosine residues in the beta subunit, initiating downstream signaling cascades, including activation of the PI3K-AKT/PKB and Ras-MAPK pathways. IGF-1 also binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. This integrin binding, along with ternary complex formation involving integrins and IGF-1R, is essential for IGF-1 signaling. IGF-1 induces the phosphorylation and activation of IGF-1R, MAPK3/ERK1, MAPK1/ERK2, and AKT1.
Gene References Into Functions
- SNX6 knockdown significantly reduced IGF-1-mediated ERK1/2 phosphorylation without affecting IGF-1R internalization. PMID: 29530981
- Increased IGF-1 production by polyploid ASCs enhanced their tumor growth-promoting capacity in vitro. PMID: 30185144
- 1,550-nm Er:Glass fractional laser treatment for androgenetic alopecia did not upregulate Wnt10A and IGF-1 mRNA. PMID: 30096107
- IGF-1 concentrations showed significant correlations with impairments on several EDI-2 subscales (drive for thinness, body dissatisfaction, interoceptive awareness, sense of ineffectiveness, interpersonal distrust, maturity fear) and SCL-90 subitems (depression, hostility, obsessivity-compulsivity, anxiety), suggesting hormonal modulation of eating behaviors. PMID: 29179911
- This review summarizes tumor-specific molecular signatures of IGF-1-mediated epithelial-mesenchymal transition in breast, lung, and gastric cancers. PMID: 30111747
- IGF-1:matrix protein interactions influence cell growth, migration, and melanoma progression through complex formation with IGF-binding proteins. PMID: 29330502
- The molecular interactions of IGF1-IGF1R binding have been characterized. PMID: 29483580
- Genetic association and nutrigenomic studies in postmenopausal Chinese women showed that dietary acid load is associated with osteoporosis, but an IGF1 SNP (rs35767) was not associated with this relationship. PMID: 30018240
- Low IGF-I levels are associated with non-alcoholic fatty liver disease. PMID: 29395967
- Low IGF1 expression is associated with retinopathy of prematurity. PMID: 29274846
- Low IGF-I levels are associated with dry eye syndrome. PMID: 29452886
- Serum IGF-1 is associated with increased muscle mass, higher bone mineral density, and improved handgrip performance in middle-aged and older adults in Taiwan. PMID: 28351218
- Insulin-like growth factor 1 (IGF1) is a direct target of miR-1827. PMID: 28387248
- Increased IGF-1 expression in cumulus cells (CCs) of women with polycystic ovary syndrome (PCOS) was inversely proportional to miR-323 expression; IGF1 3'UTR is targeted by miR-323. PMID: 30300681
- IGF-1 induced epithelial-mesenchymal transition in hepatocellular carcinoma cells through survivin regulation and downstream pathways. PMID: 29989646
- This study assessed the effects of varying concentrations of IGF-1 and BMP-7 on the osteogenic differentiation of two human MSC subtypes, isolated from reaming debris (RMSC) and iliac crest bone marrow (BMSC). PMID: 29874864
- AMH, IGF1, and leptin levels in follicular fluid are not associated with endometriosis- or fallopian tube damage-related infertility but serve as biomarkers for anovulatory infertility. PMID: 29595066
- Pituitary growth hormone secretory capacity is not related to IGF-1 levels. PMID: 29098662
- Peripheral total IGF-1 and IGFBP-3 were associated with better attention, visuospatial, and global cognitive performance, independent of gait speed. PMID: 29547749
- A CA repeat polymorphism in the P1 promoter of the IGF1 gene was associated with strength predispositions in homozygous and non-carrier groups and speed-strength aptitudes in heterozygous individuals. PMID: 29543920
- IGF1 levels in systemic lupus erythematosus patients decline with age similarly to healthy controls, exhibiting positive metabolic effects but not influencing disease activity or severity, despite a moderating effect on B, NK, and CD8+ cells. PMID: 29385899
- IGF-1 gene polymorphisms play a role in the histopathological progression of IgA nephropathy in the Chinese Han population. PMID: 29402846
- Low IGF1 levels are associated with steatosis in pituitary diseases. PMID: 29065431
- IGF-1 and IGF-2 variants with His49 and His48, respectively, showed significantly higher affinities for IR-A and IR-B than previously reported IGF-1 and IGF-2-like binders. PMID: 29608283
- The activation of SGK3 by IGF1 via class 1 and class 3 phosphatidylinositol 3-kinases has been described. PMID: 29150437
- IGF1 is associated with giant cell tumor of bone recurrence and may serve as a biomarker. PMID: 29651441
- miR-19b is decreased in PCOS granulosa cells and may inhibit granulosa cell proliferation by targeting IGF-1. PMID: 29363717
- IGF1/IGFBP3 expression is deregulated in breast cancer, correlating with stage and histologic grade. PMID: 30084561
- IGF1 levels are higher in human epithelial ovarian cancer (EOC) specimens than in benign tumors, correlating with advanced stage and liver metastasis. Tumor-associated macrophages upregulate IGF1, promoting EOC cell proliferation and migration. PMID: 29251331
- Overexpression of circHIPK3 increases IGF1 expression, while knockdown reduces it. PMID: 28738961
- Increased cGP/IGF-1 ratio is associated with obesity but not hypertension; changes in IGFBP-3 and/or cGP/IGF-1 ratio correlate with weight changes, suggesting autocrine regulation of IGF-1 by cGP in obesity. PMID: 29921371
- IGF-1 exhibits antioxidant, anti-inflammatory, and neuroprotective effects. PMID: 29975480
- The IGF-1 SNP rs6218 predicts osteosarcoma susceptibility and progression; TC and CC genotypes are associated with increased risk, later stage, and elevated risk. PMID: 29232358
- In lung cancer patients, higher TNF-alpha expression correlated with lower cancer progression risk, while IGF-1 overexpression correlated with higher risk. PMID: 27344406
- Lower IGF1 serum levels in aging are associated with lower handgrip strength and poorer physical performance but fewer recurrent falls, particularly in men. PMID: 29789408
- The R1353H IGF1R mutation is associated with extreme tall height and low serum IGF-1 levels. PMID: 29789409
- IGF-1 treatment activates protein kinase B (AKT), inhibiting autophagy via the AKT/mTOR pathway and sensitizing drug-resistant cells to 5-fluorouracil-induced apoptosis. PMID: 29257307
- TXNIP gene expression is negatively regulated by IGF1. PMID: 29339473
- In elderly women with low-energy distal radius fractures, low IGF1 is an indirect risk factor for fractures. PMID: 29178313
- Increased miR-30a-3p expression in preeclampsia placentas may contribute to the pathogenesis by targeting IGF-1 and affecting trophoblast invasion and apoptosis. PMID: 29155142
- PI3K p110 subunits may be therapeutic targets for colon cancer, and NRD1/ADAM signaling regulates lipogenesis-mediated EMT in IGF-1-stimulated colon cancer cells. PMID: 28819788
- Multiphoton microscopy demonstrated IGF-I uptake into skeletal growth plates of live mice. PMID: 28798204
- The IGF-1/IGF-1R/hsa-let-7c axis controls odonto/osteogenic differentiation of IGF-1-treated stem cells from apical papilla via JNK and p38 MAPK signaling. PMID: 27833148
- Circulating sex steroids, prolactin, IGF-I, IGFBP-3, and SHBG were evaluated in pre- and postmenopausal women. PMID: 28246273
- Serum IGFBP-1 and IGF-I levels are associated with advanced fibrosis in non-alcoholic fatty liver disease. PMID: 28927302
- The IGF1 polymorphism rs5742714 may predict renal cell carcinoma susceptibility and prognosis. PMID: 27976731
- Physical activity may improve cognitive development through modification of plasma IGF-1 or BDNF concentrations. PMID: 29094050
- Differential expression of IGF-I transcript isoforms in bladder cancer shows suppression of IGF-IEc. PMID: 29848696
- rAAV-mediated IGF-I overexpression enhances chondrogenic differentiation in peripheral blood aspirates. PMID: 28467017
- Secreted IGF-I propeptides, particularly proIGF-IA, stimulated articular chondrocyte activity and bound to heparin depending on N-glycosylation. PMID: 29174671
Database Links
Involvement In Disease
Insulin-like growth factor I deficiency (IGF1 deficiency)
Protein Families
Insulin family
Subcellular Location
Secreted.
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