Recombinant Human Leukemia inhibitory factor protein (LIF) (Active)
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Description
Production and Quality Control
Recombinant LIF is expressed in multiple systems:
Rice-derived LIF exhibits bioequivalence to E. coli-derived variants but with lower endotoxin levels, making it suitable for clinical-grade applications .
Biological Activity
LIF activates JAK/STAT, MAPK, and PI3K pathways via gp130/LIFRβ, driving diverse cellular responses :
Key Functional Assays
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M1 Myeloid Leukemia Cell Differentiation:
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Stem Cell Maintenance:
Immune Modulation
Clinical and Preclinical Applications
Challenges and Future Directions
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Cost-Efficiency: Plant-based systems (e.g., rice) reduce production costs by 90% .
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Therapeutic Targeting: Neutralizing LIF in cancers overexpressing LIF/STAT3 shows promise but requires combination with checkpoint inhibitors .
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Batch Variability: Glycosylation differences impact bioactivity; rigorous QC is critical .
Product Specs
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4.
Form
Lyophilized powder
Lead Time
5-10 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Reconstitution
We recommend centrifuging this vial briefly prior to opening to ensure the contents settle at the bottom. Reconstitute the protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting the solution at -20°C/-80°C. Our standard final concentration of glycerol is 50% and can be used as a reference.
Shelf Life
The shelf life of this product is influenced by factors such as storage conditions, buffer ingredients, temperature, and the protein's inherent stability.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquoting is necessary for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag-Free
Synonyms
CDF; Cholinergic Differentiation Factor ; D factor; DIA; Differentiation inducing factor; differentiation inhibitory activity; Differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin ; Hepatocyte stimulating factor III; HILDA; Human interleukin in DA cells; Leukemia inhibitory factor; LIF; LIF_HUMAN; Melanoma derived LPL inhibitor; Melanoma-derived LPL inhibitor; MLPLI
Datasheet & Coa
Please contact us to get it.
Expression Region
23-202aa
Mol. Weight
19.7 kDa
Protein Length
Full Length of Mature Protein
Purity
>98% as determined by SDS-PAGE.
Research Area
Immunology
Source
E.coli
Species
Homo sapiens (Human)
Target Names
Uniprot No.
Target Background
Function
Leukemia Inhibitory Factor (LIF) possesses the ability to induce terminal differentiation in leukemic cells. Its diverse functionalities encompass inducing hematopoietic differentiation in both normal and myeloid leukemia cells, promoting neuronal cell differentiation, and stimulating the synthesis of acute-phase proteins in hepatocytes.
Gene References Into Functions
- miR-181c-3p and -5p promote high-glucose-induced dysfunction in human umbilical vein endothelial cells by regulating leukemia inhibitory factor. PMID: 29605252
- Low LIF concentrations in serum and follicular fluid may contribute to disordered folliculogenesis in polycystic ovary syndrome. PMID: 29397316
- LIF activates the PI3K/AKT signal and induces an anti-inflammatory effect during neuron differentiation from human induced pluripotent stem cell-derived neural precursor cells. PMID: 29393372
- ZEB1 acts as a stem cell regulator in glioma by repressing LIF. Deletion of ZEB1 leads to increased stemness, tumorigenicity, and shortened patient survival. PMID: 28246407
- Decreased serum LIF levels may be associated with vasculopathy in systemic sclerosis (SSc). Fli1 deficiency inhibits LIF-dependent biological effects on SSc endothelial cells by suppressing the expression of LIF, LIF receptor, and gp130. PMID: 29038846
- Endometrial expression of LIF and CD34 in the pathogenesis of non-developing pregnancy can be used for evaluating the pregnancy prognosis in women of both young and older reproductive age. PMID: 29063331
- This report describes the involvement of proteins responsible for cell growth and progression and defines the LIF-mediated novel autocrine-paracrine signaling loop for cell growth arrest. PMID: 28755912
- The expression of LIF was associated with tumor size and a poorer overall survival. Microarray and quantitative real-time polymerase chain reaction assessments suggest that LIF can facilitate tumor-promoting inflammation. Results indicate that LIF plays a role in maintaining cancer stem cells in chordomas. PMID: 28247842
- This study found impaired LIF expression levels only in women with unexplained infertility, while LIF-R expression was impaired in all sub-groups of infertile women. PMID: 28432985
- Findings illustrate that DeltaNp63alpha can inhibit LIF mRNA levels by direct transcription regulation and decrease LIF mRNA stability by suppressing the expression of Lnc-LIF-AS. An inverse interaction of LIF and DeltaNp63alpha expression was also validated in clinical samples of cervical cancer, where high LIF levels were associated with poor patient survival. PMID: 28391028
- ATF3 plays a significant role in regulating human endometrial receptivity and embryo attachment in vitro via up-regulation of leukemia inhibitory factor. PMID: 28577574
- Although further studies are required to deconvolute the targets involved in LIF induction and confirm the activity of hits in more disease-relevant assays, our results have demonstrated the potential of the phenotypic approach to identify specific and chemically tractable small molecules that trigger the production of LIF in relevant cell lines. PMID: 26984928
- SNP 3951C/T of LIF may not be associated with in vitro fertilization and embryo transfer outcome in the Iranian population. PMID: 28466814
- This study indicates that LIF is implicated in the HG-mediated inhibition of osteoblast differentiation, via promoting STAT3/SOCS3 signaling. This research may provide insights into the signal pathway of HG-induced bone loss or delayed injured joint healing. PMID: 28064096
- Cytokines of the LIF/CNTF family and metabolism. PMID: 26817395
- These results demonstrate the involvement of PIM kinases in LIF-induced regulation in different trophoblastic cell lines, suggesting similar functions in primary cells. PMID: 28729093
- Data suggest that a 216-nucleotide proximal cis-element in LIF mRNA exhibits mRNA destabilizing potential; upon exposure to the carcinogen PMA (phorbol-12-myristate-13-acetate), this cis-element exhibits mRNA stabilizing activity. PMA induces nucleo-cytoplasmic translocation of both nucleolin and PCBP1, two trans-acting factors that bind to and stabilize LIF mRNA. [LIF = leukemia inhibitory factor; PCBP1 = poly(rC) binding protein 1]. PMID: 28512205
- The Leukemia inhibitory factor (LIF) - STAT3 transcription factor (STAT3) signaling pathway is systemically dysregulated in the endometrium of patients with recurrent/repeated implantation failure (RIFE). PMID: 27304912
- Endometrial expression of LIF and LIFR is significantly reduced in the epithelial cells of infertile women. PMID: 27082016
- LIF SNP T/G (rs929271) appears to be a susceptibility biomarker capable of predicting implantation efficiency and pregnancy outcomes. PMID: 26615902
- Overexpression of LIF promotes Epithelial-mesenchymal transition and contributes to cancer development. PMID: 26716902
- This review discusses the role of LIF and the recent analysis of its action on the uterine LE in regulating endometrial receptivity and implantation. PMID: 26817565
- Women with dormant genital tuberculosis were found to have decreased endometrial LIF-STAT3 signaling. PMID: 26776907
- Data show that leukemia inhibitory factor (LIF) signaling promotes chemoresistance in cholangiocarcinoma by up-regulating myeloid cell factor-1 (Mcl-1) via the phosphatidylinositol 3-kinase (PI3K)/c-akt protein (AKT)-dependent pathway. PMID: 26296968
- Despite common signal transduction mechanisms (JAK/STAT, MAPK, and PI3K), LIF can have paradoxically opposite effects in different cell types, including stimulating or inhibiting cell proliferation, differentiation, and survival. PMID: 26187859
- Taken together, this study demonstrates that LIF enhances the adhesion of trophoblastic cells to endometrial cells by up-regulating expression of integrin heterodimer alphaVbeta3 and alphaVbeta5. PMID: 26723254
- LIF plays a role in negatively regulating tumor-suppressor p53 through Stat3/ID1/MDM2 in colorectal cancers. PMID: 25323535
- The LIF/p21 signaling cascade acts as a novel tumor suppressive-like pathway in melanoma, acting downstream of TGFbeta to regulate cell cycle arrest and cell death, further highlighting new potential therapeutic strategies for the treatment of cutaneous melanoma. PMID: 25885043
- LIF is frequently overexpressed in osteosarcoma, where it promotes growth and invasion through activation of the STAT3 pathway. PMID: 26271643
- LIF structure, signaling pathway, and primary roles in the development and function of an organism are reviewed--{REVIEW}. PMID: 25879318
- LIF downregulates the autoimmune response by enhancing Treg numbers. PMID: 25514345
- LIF mediates fibroblast activation to promote an invasive tumor microenvironment. PMID: 24857661
- Data indicate that the NanoLuc-fusion strategy provides an efficient approach for preparing recombinant leukemia inhibitory factor (LIF) protein. PMID: 25179300
- Expression of LIF protects the lung from lung injury and enhances pathology during respiratory syncytial virus infection. PMID: 25277705
- This study examined the association of tubal pregnancy with leukemia inhibitory factor (LIF) and leukemia inhibitory factor receptor (LIFR) expression in oviduct tissues. PMID: 25790555
- Foxm1 plays an essential role in the LIF/Stat3-mediated mESC self-renewal and the generation of iPSCs. PMID: 24743237
- Results show that endogenous levels of Pkig reciprocally regulate osteoblast and adipocyte differentiation, and this reciprocal regulation is mediated in part by LIF. PMID: 23963683
- Increased mRNA expression of PROK1 and LIF could be one of the several abnormalities characterizing the endometrium in women experiencing recurrent pregnancy loss. PMID: 25128195
- Addition of leukemia inhibitory factor (LIF) neutralizing antibodies inhibited oligodendrocyte differentiation, indicating a crucial role of TNFR2-induced astrocyte-derived LIF for oligodendrocyte maturation. PMID: 24310780
- Data from a 3-year-old girl and other reported cases support the model where abundance of LIF in B-cell acute lymphoblastic leukemia results in leukemic infiltration of the central nervous system and development of Cushing syndrome. [CASE STUDY; REVIEW]. PMID: 23729555
- This mini review summarizes the findings related to LIF signaling and discusses the neuroprotective effects of LIF in different models. PMID: 24664722
- Data suggest LIF/LIF receptor (alpha subunit) signal transduction facilitates blastocyst implantation or development of tubal pregnancy by stimulating blastocyst adhesion and outgrowth/proliferation of placenta or Fallopian tube epithelial cells. PMID: 24074901
- Polyethylene glycated leukemia inhibitory factor antagonist inhibits human blastocyst implantation and triggers apoptosis by down-regulating embryonic AKT1. PMID: 23876532
- NPC patients had increased serum levels of LIF. Higher LIF levels correlated with local tumor recurrence. Xenograft mouse studies showed that LIF critically contributes to NPC tumor growth and radioresistance. PMID: 24270418
- This study found lower expression of LIF in the endometrium in unexplained infertile women with multiple implantation failures compared to fertile women; data suggest that the initial lower expression of LIF in the proliferative phase may be one of the causes for multiple failure of implantation. PMID: 23541977
- This research describes a novel expression and purification protocol for the production of recombinant hLIF. PMID: 23628981
- In summary, these studies show that LIF is an important factor in melanoma progression. PMID: 23831429
- Data show that the expressions of ER-alpha, PR, LIF, VEGF, iNOS, and CB1 in the fallopian tube and chorionic villi of tubal pregnancy were not altered by exposure to levonorgestrel emergency contraception. PMID: 23687977
- LIF is a contraction-induced myokine, potentially acting in an autocrine or paracrine fashion to promote skeletal muscle satellite cell proliferation. PMID: 21527666
- No correlation was found between pinopodes development stage and LIF expressions in the endometrium. PMID: 22252755
Database Links
Protein Families
LIF/OSM family
Subcellular Location
Secreted.
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