Recombinant Human Tumor necrosis factor receptor superfamily member 13C protein (TNFRSF13C), partial (Active)
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Description
Introduction to Recombinant Human TNFRSF13C Protein
The recombinant human TNFRSF13C protein (BAFF receptor) is a truncated, bioactive form of the tumor necrosis factor receptor superfamily member 13C. This partial protein (typically spanning amino acids 7–71) is engineered for research applications to study BAFF-mediated signaling, B-cell development, and immune regulation. It is produced in mammalian or bacterial expression systems and retains functional binding activity with BAFF (TNFSF13B). Key applications include investigating autoimmune diseases, B-cell malignancies, and immunodeficiency disorders .
Production Methods
Recombinant TNFRSF13C is synthesized using plasmid constructs encoding specific amino acid regions (e.g., 7–71) and expressed in:
| Host System | Tag | Purity | Endotoxin Level | Source |
|---|---|---|---|---|
| HEK-293 cells | His, Fc, or Avi | >95% (SDS-PAGE) | <1.0 EU/μg | |
| E. coli | None | >95% (SDS-PAGE) | <1.0 EU/μg |
Key Features:
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Tags: Fc, His, or Avi tags enhance solubility and purification.
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Activity Validation: ELISA confirms binding to BAFF with EC₅₀ values of 9.943–15.72 ng/ml (unlabeled BAFF) and 0.2699–0.5613 ng/ml (biotinylated BAFF) .
Binding and Survival Assays
| Assay Type | Target | EC₅₀ | Application | Source |
|---|---|---|---|---|
| ELISA | TNFSF13B | 0.27–15.7 ng/ml | BAFF-R interaction specificity | |
| Splenocyte Survival | BAFF-induced B-cell survival | 1.0–5.0 μg/ml | In vitro B-cell survival studies |
Mechanism: BAFF-R binds BAFF via its extracellular domain, activating NF-κB and MAPK pathways to promote B-cell maturation and survival .
Autoimmune Diseases
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Common Variable Immunodeficiency (CVID): Mutations in TNFRSF13C (e.g., del89–96) impair BAFF-R expression, leading to reduced transitional B-cell maturation and hypogammaglobulinemia .
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Systemic Lupus Erythematosus (SLE): Elevated BAFF levels correlate with autoreactive B-cell survival .
B-Cell Malignancies
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Lymphomas: BAFF-R is overexpressed in follicular lymphoma and mantle cell lymphoma, making it a therapeutic target .
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Antibody Development: Humanized anti-BAFF-R antibodies (e.g., afucosylated variants) show efficacy in preclinical models of B-cell cancers .
Genetic Variants
| Variant | Effect | Disease Association | Source |
|---|---|---|---|
| H159Y | Altered BAFF binding | Severe COVID-19 susceptibility | |
| del89–96 | Transmembrane domain truncation | CVID-like immunodeficiency |
Clinical Relevance: BAFF-R deficiency results in:
Comparative Analysis of Recombinant TNFRSF13C Variants
| Parameter | HEK-293-Expressed (7–71 aa) | E. coli-Expressed (7–76 aa) | Fc-Chimera |
|---|---|---|---|
| Purity | >95% | >90% | >95% |
| Endotoxin | <1.0 EU/μg | <1.0 EU/μg | <1.0 EU/μg |
| Tag | His, Fc | None | Fc |
| Activity | BAFF binding (EC₅₀: 0.27–15.7 ng/ml) | BAFF binding (EC₅₀: N/A) | BAFF blocking (ED₅₀: 20–80 ng/ml) |
Product Specs
Buffer
Lyophilized from a 0.2 µm filtered 20 mM PB, pH 8.0, 500 mM NaCl solution.
Description
This Recombinant Human TNFRSF13C (CD268) protein is a valuable tool for cancer research. TNFRSF13C, also known as Tumor necrosis factor receptor superfamily member 13C, plays a critical role in immune regulation and is implicated in various cancer-related processes.
Our TNFRSF13C protein is produced using an E. coli expression system and encompasses amino acids 1 to 76, representing a partial length of the TNFRSF13C sequence. It is tag-free, ensuring its native conformation and eliminating any potential interference in downstream applications. With a purity exceeding 95% and minimal endotoxin contamination, our TNFRSF13C protein provides reliable and consistent results.
Form
Lyophilized powder
Lead Time
5-10 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Reconstitution
We recommend centrifuging this vial briefly prior to opening to bring the contents to the bottom. Please reconstitute the protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers can use this as a reference.
Shelf Life
The shelf life is dependent on several factors, including storage conditions, buffer ingredients, storage temperature, and the protein's intrinsic stability.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquoting is necessary for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag-Free
Synonyms
B cell activating factor receptor; B-cell-activating factor receptor; BAFF R; BAFF receptor; BAFF-R; BAFFR; BLyS receptor 3; BlySR3; BR 3; BR3; BROMIX; CD 268; CD268; CD268 antigen; CVID4; MGC138235; OTTHUMP00000028746; Prolixin; TNFRSF 13C; Tnfrsf13c; TR13C_HUMAN; Tumor necrosis factor receptor subunit member 13C; Tumor necrosis factor receptor superfamily member 13C
Datasheet & Coa
Please contact us to get it.
Expression Region
1-76aa
Mol. Weight
7.8 kDa
Protein Length
Partial
Purity
>95% as determined by SDS-PAGE.
Research Area
Cancer
Source
E.coli
Species
Homo sapiens (Human)
Target Names
Uniprot No.
Target Background
Function
B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response.
Gene References Into Functions
- The B-cell receptor BR3 modulates cellular branching via Rac1 during neuronal migration PMID: 27436754
- Expression patterns of BAFF and its receptor BAFF-R differ according to lupus nephritis class. PMID: 29087261
- Up-regulated expression is observed in intractable temporal lobe epilepsy PMID: 28441631
- Inhibition of ADAM10 augments BAFF-dependent survival of primary human B cells, whereas inhibition of ADAM17 increases BAFFR expression levels. PMID: 28249164
- Relationships between serum BAFF and BBR expression [(BAFFR, calcium signal modulating cyclophilic ligand interactor (TACI) and B cell maturation antigen (BCMA)] were determined on B cell subsets, defined using immunoglobulin (Ig)D/CD38. Twenty pre-RTX and 18 rheumatoid arthritis patients relapsing after B cell depletion were included. PMID: 28800164
- Among the BAFF receptors in a cohort of rheumatoid arthritis (RA) patients, the AA have shown, by fluorescence activated cell sorter (FACS) analysis of median fluorescence intensity (MFI), that transmembrane activator and calcium-modulating cyclophilin ligand interactor (TACI) and B cell maturation antigen (BCMA) do not change PMID: 28834574
- The expression levels of serum BAFF and the three receptors (TACI, BCMA and BAFF-R) in non-Hodgkin lymphoma patients were significantly higher than in healthy controls. PMID: 28028945
- Variants in the BAFF-R gene are associated with chronic lymphocytic leukemia. PMID: 27468724
- BAFF-R, as the principal receptor of BAFF, not only decreased the apoptosis of B cells and CD8+ T cells by upregulating the expression of Bcl-2 and BclxL, but also promoted B-cell proliferation in immune thrombocytopenia. PMID: 26749059
- Genetic polymorphism contributes to the pathogenesis of primary antibody deficiency PMID: 26613719
- BAFF and BAFF-R are expressed in the thyrocytes derived from patients with either autoimmune thyroid disorders or multinodular goiter, as well in the infiltrating immune cells of Graves' disease and Hashimoto's thyroiditis PMID: 26214745
- There is an increased prevalence of the BAFF-R His159Tyr mutation in patients with Sjogren's syndrome (SS), particularly in those with SS complicated by MALT lymphoma whose disease onset occurred at a younger age. PMID: 26097183
- Expression of mutant caspase-9 correlated with a downregulation of BAFFR (B-cell-activating factor belonging to the TNF family (BAFF) receptor) in B cells and ICOS (inducible T-cell costimulator) in T cells. PMID: 25569260
- Variants of TNFRSF13C were associated with common variable immunodeficiency. PMID: 26122175
- Negative expression of BAFF-R, but not of BAFF, could be an independent risk factor for progression-free survival and Overall survival in patients with diffuse large B-cell lymphoma treated with standard R-CHOP. PMID: 26327569
- The common TLR4-D299G, TLR4-T399I and BAFFR-P21R polymorphisms provide the carriers with a protective advantage against ICU-acquired sepsis; this finding was more profound for medical patients compared to trauma or surgical ones PMID: 25454804
- Data show significant differences in expression of tumour necrosis factor family (BAFF) receptors BAFF-R, BCMA and TACI in patients with and without anti-Jo-1 or anti-Ro52/anti-Ro60 autoantibodies. PMID: 25301447
- In view of the restricted expression of the BAFF-R on normal cells and the multiple anti-pre-B ALL activities stimulated by this antibody, a further examination of its use for treatment of pre-B ALL is warranted. PMID: 24825858
- Availability of BAFF determines BAFF-R and TACI expression on B cells in common variable immunodeficiency. PMID: 24809296
- Our study demonstrates that BR3 is involved in the survival of cultured epithelial cells due to an autocrine effect of BAFF. PMID: 24602383
- BAFF-R, but not BAFF, may have a role in progression-free survival and overall survival in follicular lymphoma PMID: 23272079
- BLyS and its receptors are expressed by B lymphocytes in the peripheral blood and the bone marrow of patients with multiple myeloma. PMID: 23276925
- P21R/H159Y TNFRSF13C compound heterozygous mutation and P21R heterozygous mutations were detected in Turkish patients with common variable immunodeficiency. PMID: 22699762
- BAFF-R expression is tightly regulated during B-cell development in mouse and human, and this expression is correlated with positive selection. PMID: 22028296
- It was also found that NF-kappaB was an important transcription factor involved in regulating BAFF-R expression through one NF-kappaB binding site in the BAFF-R promoter PMID: 21607696
- Soluble BAFF levels inversely correlate with peripheral B cell numbers and the expression of BAFF receptors. PMID: 22124120
- The activation profile of diffuse large B-cell lymphomas/posttransplantation lymphoproliferative disorders was not associated with BAFF/BAFF-R expression, whereas nuclear p52 activation might be linked to Epstein-Barr virus. PMID: 21871426
- The human BAFF-R gene might be regulated via a transcriptional event through one putative NF-kappaB site on the BAFF-R gene promoter. PMID: 21744373
- Reduced expression is observed via inhibition of the NF-KappaB pathway in B cells of rheumatoid arthritis patients PMID: 21515993
- Primary leukemia B-cell precursors aberrantly express receptors of the BAFF-system, BAFF-R, BCMA, and TACI PMID: 21687682
- This is the first study presenting together the TNFSF members APRIL, BAFF, TWEAK and their receptors in different areas of normal renal tissue and renal cell carcinoma. PMID: 21483105
- BLyS and its receptors might have a potential role in the growth and survival of malignant plasma cells. PMID: 19731825
- Results describe the mechanisms underlying aberrant BAFF-R expression in precursor B acute lymphoblastic leukemia (precursor B-ALL) and mature B chronic lymphocytic leukemia (CLL). PMID: 21099364
- BAFF-R was rather specifically related to low growth activity of germinal center B-cell-like -type diffuse large B-cell lymphoma of nodal origin. PMID: 21123970
- An inverse correlation between BAFF and APRIL in Kawasaki disease is reversed by IVIG treatment PMID: 20945608
- Elevated plasma BAFF and reduced BAFF receptor 3 (BR3) protein expression on peripheral B cells could act as biomarkers for active disease in systemic lupus erythematosus patients PMID: 20974656
- A novel lymphoma-associated mutation in human BAFF-R that results in NF-kappaB activation and reveals TRAF6 as a necessary component of normal BAFF-R signaling. PMID: 21041452
- Data from BAFF-R-expressing cells suggested potential regulatory sites in the TNFRSF13C promoter region. PMID: 20554963
- This report is the first showing universal expression of BAFF-R by pre-B ALL cells. PMID: 20460528
- IFN-gamma and the NF-kappaB pathway could be involved in regulating the transcription and mRNA expression of the BAFF-R gene. PMID: 20230666
- The mechanism of transcriptional regulation of BAFF-R PMID: 20025535
- BAFF-R is a receptor for the TNF family member ligand, BAFF [review] PMID: 12456020
- BAFFR-mediated NF-kappa B activation and IL-10 production in B cells is downregulated by TNFR-associated factor-3. PMID: 12471121
- Crystal structure and interaction with BAFF protein PMID: 12715002
- BAFF/BLyS receptor 3 comprises a minimal TNF receptor-like module that encodes a highly focused ligand-binding site. PMID: 12755599
- Expression of BCMA, TACI, and BAFF-R by multiple myeloma cells support cell growth and survival. PMID: 14512299
- This study reports the crystal structure of a 24-residue fragment of the cytoplasmic portion of BAFF-R bound in complex with TRAF3. PMID: 15585864
- The PVPAT sequence of BAFFR not only functions as a key signaling motif of BAFFR but also determines its signaling specificity in the induction of the noncanonical NF-kappaB pathway PMID: 15644327
- Amino acid sequence of genomic DNA from blood of common variable immunodeficiency patients; mutations may result in humoral immunodeficiency PMID: 16160919
- BAFF-R is expressed on most mature B cells and B-cell lymphoproliferative disorders. PMID: 16226112
Database Links
Involvement In Disease
Immunodeficiency, common variable, 4 (CVID4)
Subcellular Location
Membrane; Single-pass type III membrane protein.
Tissue Specificity
Highly expressed in spleen and lymph node, and in resting B-cells. Detected at lower levels in activated B-cells, resting CD4+ T-cells, in thymus and peripheral blood leukocytes.
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