SCGB1A1 Human, His
Description
Production and Quality Control
Expression System:
Purification:
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Proprietary chromatographic techniques (e.g., immobilized metal affinity chromatography for His-tagged versions) .
Quality Metrics:
| Parameter | Specification |
|---|---|
| Purity | >90% (SDS-PAGE, RP-HPLC) |
| Endotoxin Levels | <1.0 EU/μg |
| Activity | ED₅₀ <5.0 μg/mL in cell adhesion assays |
Biological Functions and Mechanisms
SCGB1A1 Human, His exhibits key bioactivities mirroring the native protein:
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Anti-Inflammatory Effects:
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Anti-Tumorigenic Activity:
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Immunomodulation:
Research Applications
In Vitro Studies:
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Cell Culture: Used to study macrophage polarization (e.g., blunting LPS-induced cytokine storms) .
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Drug Discovery: Screens for compounds targeting SCGB1A1-related pathways in asthma or cancer .
In Vivo Models:
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Knockout Mice: Scgb1a1⁻/⁻ mice exhibit exacerbated lung inflammation and fibrosis, validating therapeutic potential .
Diagnostic Development:
Key Research Findings
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Asthma and COPD: Reduced SCGB1A1 levels in patient sera predict airway hyperresponsiveness and disease progression .
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Cancer: High SCGB1A1 expression in HNSCC tumors correlates with prolonged survival and chemoresistance .
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Infection Models: Exogenous SCGB1A1 suppresses viral/bacterial-induced cytokine surges (e.g., IL-8, MCP-1) .
Limitations and Considerations
Product Specs
Product Science Overview
Uteroglobin is a multifunctional protein with several biological properties:
The protein plays several crucial roles in the body:
- Anti-inflammatory: By inhibiting phospholipase A2, uteroglobin helps reduce inflammation.
- Immune Modulation: It has been shown to modulate immune responses, potentially protecting tissues from damage during inflammation.
- Reproductive Health: In the uterus, uteroglobin is involved in reproductive processes, although its exact role is still being studied .
Recombinant human uteroglobin with a His tag is a laboratory-produced version of the protein. It is typically expressed in HEK 293 cells and purified to a high degree of purity (>97%) with low endotoxin levels (<1 EU/µg) . The His tag facilitates purification and detection of the protein in various experimental applications.
