SDCBP Human

Syndecan Binding Protein Human Recombinant
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Cat. No.
BT7336
Source
Escherichia Coli.
Synonyms
SYCL, syntenin-1, Syndecan-binding protein 1, Scaffold protein Pbp1, MDA-9, Melanoma differentiation-associated protein 9, TACIP18, Pro-TGF-alpha cytoplasmic domain-interacting protein 18.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Molecular Structure and Function

SDCBP is a 298-amino-acid protein with a molecular mass of 33 kDa, featuring two PDZ domains that mediate interactions with transmembrane proteins like syndecans, ephrins, and interleukin-5 receptor subunits . These interactions enable SDCBP to:

  • Facilitate cytoskeletal-membrane organization and protein trafficking .

  • Regulate exosome biogenesis through partnerships with syndecans and PDCD6IP .

  • Modulate transcription factor activation, including SMAD2/3 in TGF-β signaling and YAP1 in Hippo pathways .

Biochemical properties:

  • Purity: >85% (SDS-PAGE) .

  • Binding partners: Validated interactions with YAP1, TGFR1, and syndecans .

Expression and Localization

SDCBP exhibits widespread tissue expression, with elevated levels in:

  • Cancer tissues: Strong immunohistochemical staining in lung, colorectal, and breast cancers .

  • Subcellular compartments: Localizes to adherens junctions, focal adhesions, and exosomes .

Therapeutic Potential

Preclinical studies highlight SDCBP as a target:

  • siRNA/sgRNA knockdown: Reduces tumor growth in HNSCC and PDAC xenografts .

  • Small-molecule inhibitors: ZnPT decreases SDCBP-YAP1 binding, suppressing metastasis in murine PDAC models .

Product Specs

Introduction
SDCBP, also known as syntenin-1, is a multifunctional adapter protein found inside cells. Its primary function involves connecting various proteins, thereby influencing cell signaling and behavior. Notably, SDCBP possesses multiple PDZ domains that interact with the FYA (phenylalanine-tyrosine-alanine) sequence present at the C-terminus of syndecans. Through these interactions, SDCBP plays a crucial role in: - Formation of protein complexes at the plasma membrane - Regulation of B-cell development - Activation of transcription factors - Intracellular trafficking and cell surface targeting of proteins - Synaptic transmission - Axonal outgrowth
Description
Recombinant SDCBP protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 318 amino acids (residues 1-298). This protein has a molecular weight of 34.6 kDa. For purification and detection purposes, a 20 amino acid His-tag is fused to the N-terminus. The purification process involves proprietary chromatographic techniques to ensure high purity.
Physical Appearance
The product is a sterile and filtered solution, appearing clear and without any visible particles.
Formulation
The SDCBP protein is provided as a solution with a concentration of 0.5 mg/ml. It is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 40% glycerol. This formulation helps to maintain the protein's stability and activity.
Purity
The purity of the SDCBP protein is greater than 90%, as determined by SDS-PAGE analysis.
Stability
For short-term storage (2-4 weeks), the SDCBP protein solution should be kept at 4°C. For long-term storage, it is recommended to store the protein at -20°C. Avoid repeated freeze-thaw cycles to prevent protein degradation.
Synonyms
SYCL, syntenin-1, Syndecan-binding protein 1, Scaffold protein Pbp1, MDA-9, Melanoma differentiation-associated protein 9, TACIP18, Pro-TGF-alpha cytoplasmic domain-interacting protein 18.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSLYPSLEDL KVDKVIQAQT AFSANPANPA ILSEASAPIP HDGNLYPRLY PELSQYMGLS LNEEEIRANV AVVSGAPLQG QLVARPSSIN YMVAPVTGND VGIRRAEIKQ GIREVILCKD QDGKIGLRLK SIDNGIFVQL VQANSPASLV GLRFGDQVLQ INGENCAGWS SDKAHKVLKQ AFGEKITMTI RDRPFERTIT MHKDSTGHVG FIFKNGKITS IVKDSSAARN GLLTEHNICE INGQNVIGLK

Product Science Overview

Structure and Function

Syndecan-4 has two conserved cytoplasmic domains and divergent extracellular portions, except for heparan sulfate attachment sites . It plays a crucial role in cell adhesion, migration, and proliferation by interacting with various growth factors, cytokines, and extracellular matrix components .

Recombinant Production

Recombinant human Syndecan-4 protein is typically produced using a mouse myeloma cell line, NS0-derived human Syndecan-4 protein, with a C-terminal 6-His tag . The protein is purified to a high degree, with a purity greater than 90% as determined by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining .

Applications

Recombinant Syndecan-4 is used in various research applications, including:

  • Functional ELISA: It is measured by its binding ability in a functional ELISA. Immobilized recombinant human Syndecan-4 at 500 ng/mL can bind recombinant human FGF-basic with a linear range of 0.1-10 ng/mL .
  • Cell Culture: It is used in cell or tissue culture to study cell adhesion, migration, and proliferation .
  • Cancer Research: Syndecan-4 is involved in tumor microenvironment modulation, cancer metastasis, and drug resistance .
Storage and Stability

Recombinant Syndecan-4 protein is typically lyophilized from a 0.2 μm filtered solution in PBS. It is recommended to store the protein at -20 to -70 °C to maintain its stability. After reconstitution, it can be stored at 2 to 8 °C for up to one month under sterile conditions .

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