Recombinant Proteins

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SDC1 Human

Syndecan-1 Human Recombinant

Recombinant human SDC1, produced in E. coli, is a single polypeptide chain consisting of 262 amino acids (residues 18-254) with a molecular weight of 27kDa. Note that the molecular size on SDS-PAGE might appear higher. The SDC1 protein is fused to a 25 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT7078
Source
E.coli.
Appearance
Clear, colorless solution that has been sterilized by filtration.

SDC2 Human

Syndecan-2 Human Recombinant

This product consists of the recombinant human SDC2 protein produced in Sf9 Baculovirus cells. It is a single, glycosylated polypeptide chain with a molecular weight of 15 kDa (appears at approximately 18-28 kDa on SDS-PAGE). The protein sequence encompasses amino acids 19-144a.a. and includes a 6-amino acid His tag at the C-terminus. Purification is achieved using proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT7172
Source

Sf9, Baculovirus cells.

Appearance
A clear, colorless solution that has been sterilized by filtration.

SDC4 Human

Syndecan-4 Human Recombinant

Recombinant human SDC4, produced in E. coli, is an unglycosylated polypeptide chain comprising 128 amino acids (residues 19-145a.a.) with a molecular weight of 13.9 kDa. The protein is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT7242
Source
Escherichia Coli.
Appearance
Clear, colorless, and sterile-filtered solution.

SDCBP2 Human

Syndecan Binding Protein 2 Human Recombinant

Recombinant SDCBP2 Human, produced in E.Coli, is a single, non-glycosylated polypeptide chain consisting of 315 amino acids (1-292 a.a.). With a molecular mass of 34.0kDa, it features a 23 amino acid His-tag at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT8090
Source
Escherichia Coli.
Appearance
A clear solution that has been sterilized through filtration.

SDCBP Human

Syndecan Binding Protein Human Recombinant

Recombinant SDCBP protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 318 amino acids (residues 1-298). This protein has a molecular weight of 34.6 kDa. For purification and detection purposes, a 20 amino acid His-tag is fused to the N-terminus. The purification process involves proprietary chromatographic techniques to ensure high purity.
Shipped with Ice Packs
Cat. No.
BT7336
Source
Escherichia Coli.
Appearance
The product is a sterile and filtered solution, appearing clear and without any visible particles.
Definition and Classification

Syndecans are a family of transmembrane heparan sulfate proteoglycans (HSPGs) that play crucial roles in cell signaling, adhesion, and migration. They are characterized by their single transmembrane domain and the presence of heparan sulfate and chondroitin sulfate chains on their extracellular domain . The syndecan family consists of four members: Syndecan-1, Syndecan-2, Syndecan-3, and Syndecan-4 . These proteins are classified based on their sequence homology and structural features .

Biological Properties

Key Biological Properties: Syndecans are involved in various cellular processes, including cell adhesion, migration, and proliferation . They interact with a wide range of ligands, such as growth factors, cytokines, and extracellular matrix proteins .

Expression Patterns: Syndecans are expressed in a tissue-specific manner. For example, Syndecan-1 is predominantly found in epithelial cells, while Syndecan-2 is expressed in fibroblasts and endothelial cells .

Tissue Distribution: Syndecans are distributed across various tissues. Syndecan-1 is primarily located in epithelial tissues, Syndecan-2 in connective tissues, Syndecan-3 in neural tissues, and Syndecan-4 in a variety of tissues, including muscle and skin .

Biological Functions

Primary Biological Functions: Syndecans are involved in cell-cell and cell-matrix interactions, which are essential for tissue development and repair . They also play a role in the regulation of cell growth and differentiation .

Role in Immune Responses and Pathogen Recognition: Syndecans participate in immune responses by mediating the interaction between leukocytes and endothelial cells, facilitating leukocyte extravasation . They also bind to pathogens, aiding in their recognition and clearance by the immune system .

Modes of Action

Mechanisms with Other Molecules and Cells: Syndecans interact with various molecules, including growth factors, cytokines, and extracellular matrix proteins, through their heparan sulfate chains . These interactions trigger downstream signaling pathways that regulate cellular behavior .

Binding Partners and Downstream Signaling Cascades: Syndecans bind to multiple partners, such as fibroblast growth factors and vascular endothelial growth factor . These interactions activate signaling cascades involving pathways like Wnt, integrin, and receptor tyrosine kinases .

Regulatory Mechanisms

Regulatory Mechanisms: The expression and activity of syndecans are regulated at multiple levels. Transcriptional regulation involves various transcription factors, including Hox and MyoD family members . Post-translational modifications, such as phosphorylation and glycosylation, also play a critical role in modulating syndecan function .

Applications

Biomedical Research: Syndecans are studied for their roles in cancer, inflammation, and wound healing . They serve as biomarkers for disease progression and therapeutic targets .

Diagnostic Tools: Syndecan levels can be measured to diagnose and monitor diseases, such as cancer and cardiovascular conditions .

Therapeutic Strategies: Syndecans are targeted in therapies aimed at modulating their interactions with ligands to treat diseases like cancer and fibrosis .

Role in the Life Cycle

Development to Aging and Disease: Syndecans are involved in various stages of the life cycle. During development, they regulate cell proliferation and differentiation . In adulthood, they maintain tissue homeostasis and repair . Dysregulation of syndecan expression is associated with aging and diseases, such as cancer and neurodegenerative disorders .

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