Recombinant Proteins

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EDAR Human, Sf9

Ectodysplasin A Receptor Human Recombinant, Sf9

EDAR, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain consisting of 413 amino acids (27-187a.a.) with a molecular mass of 45.6 kDa. Note that on SDS-PAGE, the molecular size will appear at approximately 40-57 kDa. This EDAR protein is expressed with a 249 amino acid hIgG-His-tag at the C-Terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT1134
Source

Sf9, Baculovirus cells.

Appearance
Sterile filtered colorless solution.

EDA2R Human

Ectodysplasin A2 Receptor Human Recombinant

Recombinant EDA2R, of human origin, is produced in E. coli. This protein is a single, non-glycosylated polypeptide chain comprising 161 amino acids (with a sequence spanning from amino acid position 1 to 138) and has a molecular weight of 17.7 kDa. The EDA2R protein is expressed with a 23 amino acid His-tag fused to its N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT907
Source
Escherichia Coli.
Appearance
The product is a clear solution that has been sterilized by filtration.

EDA2R Human, Sf9

Ectodysplasin A2 Receptor Human Recombinant, Sf9

Recombinant human EDA2R, produced in Sf9 insect cells (Baculovirus expression system), is a single, glycosylated polypeptide chain. It consists of 380 amino acids (1-138a.a.), including a 242 amino acid hIgG-His tag at the C-terminus, and has a molecular mass of 42.5 kDa. However, its apparent molecular size on SDS-PAGE is approximately 40-57 kDa. The protein is purified using proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT976
Source

Sf9, Baculovirus cells.

Appearance
Clear, sterile-filtered solution.

EDAR Human

Ectodysplasin A Receptor Human Recombinant

Recombinant human EDAR, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 445 amino acids (27-448 a.a.). It has a molecular weight of 48.2kDa. The protein includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT1071
Source
Escherichia Coli.
Appearance
Clear, sterile-filtered solution.
Definition and Classification

Ectodysplasin A (EDA) is a protein encoded by the EDA gene, belonging to the tumor necrosis factor (TNF) family. It plays a crucial role in the development of ectodermal tissues such as skin, hair, teeth, and certain glands . EDA exists in two main splice variants: EDA-A1 and EDA-A2, which bind to different receptors .

Biological Properties

Key Biological Properties: EDA is a transmembrane protein that functions as a homotrimer. It is involved in the formation of various ectodermal derivatives during prenatal development .

Expression Patterns: EDA is primarily expressed in ectodermal tissues, including skin, hair follicles, and glands .

Tissue Distribution: EDA is distributed in tissues derived from the ectoderm, such as the epidermis, hair follicles, teeth, and sweat glands .

Biological Functions

Primary Biological Functions: EDA is essential for the morphogenesis of skin appendages, including hair, teeth, and glands . It also plays a role in the development of feathers in birds and scales in fish .

Role in Immune Responses and Pathogen Recognition: EDA signaling has been implicated in the regulation of immune responses, particularly in the context of inflammation and pathogen recognition .

Modes of Action

Mechanisms with Other Molecules and Cells: EDA exerts its effects by binding to its receptors, EDAR and EDA2R. EDA-A1 binds to EDAR, while EDA-A2 binds to EDA2R .

Binding Partners and Downstream Signaling Cascades: Upon binding to its receptors, EDA activates the NF-κB signaling pathway through the recruitment of the EDAR-associated death domain (EDARADD) adapter protein . This signaling cascade leads to the transcription of target genes involved in the development of ectodermal tissues .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of EDA are regulated at multiple levels, including transcriptional regulation and post-translational modifications .

Transcriptional Regulation: The EDA gene is regulated by various transcription factors that control its expression during development .

Post-Translational Modifications: EDA undergoes proteolytic processing to generate its active form, which can then bind to its receptors and initiate signaling .

Applications

Biomedical Research: EDA and its signaling pathway are studied extensively in the context of developmental biology and genetic disorders .

Diagnostic Tools: Mutations in the EDA gene are associated with ectodermal dysplasias, which can be diagnosed through genetic testing .

Therapeutic Strategies: Recombinant EDA protein has been explored as a potential therapeutic strategy for treating ectodermal dysplasias .

Role in the Life Cycle

Development: EDA is critical for the proper development of ectodermal tissues during embryogenesis .

Aging and Disease: Dysregulation of EDA signaling has been linked to various diseases, including ectodermal dysplasias, cancer, and metabolic disorders .

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