Recombinant Proteins

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TUBG1 Human

Tubulin Gamma 1 Human Recombinant

Recombinant human TUBG1, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 471 amino acids (specifically, amino acids 1 to 451) and has a molecular weight of 53.3 kDa. The protein features a 20 amino acid His-tag at the N-terminus and undergoes purification using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20972
Source
E.coli.
Appearance
A sterile, colorless solution that has been filtered.

TUBG1 Human, Sf9

Tubulin Gamma 1 Human Recombinant, Sf9

Produced in Sf9 insect cells, TUBG1 is a single, glycosylated polypeptide chain consisting of 457 amino acids (1-451 a.a.) with a molecular weight of 51.9kDa. It is expressed with a C-terminal 6-amino acid His tag and purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT21057
Source

Sf9, Insect cells.

Appearance
Sterile filtered, colorless solution.
Definition and Classification

Tubulin gamma (γ-tubulin) is a member of the tubulin superfamily, which also includes α-tubulin and β-tubulin. It plays a crucial role in the nucleation and polar orientation of microtubules, primarily found in centrosomes and spindle pole bodies . γ-Tubulin is essential for the formation of the γ-tubulin ring complex (γ-TuRC), which is critical for microtubule nucleation .

Biological Properties

Key Biological Properties: γ-Tubulin is highly conserved across eukaryotic species and is essential for microtubule organization . It is involved in the formation of spindle, phragmoplast, and cortical microtubule arrays .

Expression Patterns and Tissue Distribution: γ-Tubulin is ubiquitously expressed in eukaryotic cells, with high concentrations in centrosomes and spindle pole bodies . In plants, it is localized with all microtubule arrays and in nuclei .

Biological Functions

Primary Biological Functions: γ-Tubulin is a key player in microtubule nucleation, which is essential for cell division and intracellular organization . It also plays a role in the coordination of late mitotic events and mitotic checkpoint control .

Role in Immune Responses and Pathogen Recognition: While γ-tubulin’s primary functions are related to microtubule organization, its role in immune responses and pathogen recognition is less well-documented. However, its involvement in cell division and intracellular transport indirectly supports immune cell functions .

Modes of Action

Mechanisms with Other Molecules and Cells: γ-Tubulin interacts with other proteins to form the γ-tubulin ring complex (γ-TuRC), which serves as a template for microtubule nucleation . It also interacts with centrosomal proteins to organize microtubules .

Binding Partners and Downstream Signaling Cascades: γ-Tubulin binds to accessory proteins such as Spc97 and Spc98 to form the γ-tubulin small complex (γ-TuSC), which is essential for microtubule nucleation . It also interacts with proteins involved in cell cycle regulation and stress signaling .

Regulatory Mechanisms

Regulatory Mechanisms: The expression and activity of γ-tubulin are regulated by various mechanisms, including transcriptional regulation and post-translational modifications . γ-Tubulin is part of a multi-subunit ring complex that promotes microtubule nucleation and caps the minus ends of microtubules .

Transcriptional Regulation and Post-Translational Modifications: γ-Tubulin undergoes various post-translational modifications that affect its function and interactions with other proteins . These modifications include phosphorylation, which can regulate its activity and localization .

Applications

Biomedical Research: γ-Tubulin is widely studied in the context of cell division and cancer research. Its role in microtubule nucleation makes it a target for anti-cancer drugs .

Diagnostic Tools and Therapeutic Strategies: γ-Tubulin antibodies are used in diagnostic tools to study microtubule organization and cell division . Targeting γ-tubulin and its associated complexes is a potential therapeutic strategy for cancer treatment .

Role in the Life Cycle

Role Throughout the Life Cycle: γ-Tubulin is essential for proper cell division and development. Disruption of γ-tubulin function can lead to defects in spindle formation and cell division, affecting development and growth . It also plays a role in aging and disease, particularly in cancer, where its dysregulation can contribute to tumorigenesis .

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