Recombinant Proteins

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PTX2 Mouse

Pentraxin-2 Mouse Recombinant

Recombinant Mouse PTX2, produced in Sf9 insect cells, is a single glycosylated polypeptide chain consisting of 210 amino acids (residues 21-224). It has a molecular weight of 24.6 kDa, but on SDS-PAGE, it appears between 28-40 kDa due to glycosylation. This PTX2 protein is fused to a 6-amino acid His-tag at its C-terminus and is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT3250
Source

HEK293.

Appearance
A clear solution that has been sterilized by filtration.

PTX3 Human

Pentraxin-3 Human Recombinant

Recombinant Human PTX3, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 401 amino acids (18-381 a.a) with a molecular weight of 44.4 kDa. This PTX3 protein is fused to a 37 amino acid His Tag at the N-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT3297
Source
Escherichia Coli.
Appearance
Sterile, colorless solution.

PTX3 Human, HEK

Pentraxin-3 Human Recombinant, HEK

Recombinant Human Pentraxin-3, produced in HEK cells, is a single, glycosylated polypeptide chain. It spans from Glu18 to Ser381 (total of 370 amino acids), resulting in a calculated molecular mass of 41kDa. The protein includes a 6 amino acid His-tag fused at the C-terminus.
Shipped with Ice Packs
Cat. No.
BT3352
Source
HEK 293.
Appearance
White, lyophilized powder after filtration.
Definition and Classification

Pentraxin is a family of proteins characterized by a conserved 200 amino acid domain with a distinctive pentameric structure. They are classified into two main groups:

  • Short Pentraxins: Includes C-reactive protein (CRP) and serum amyloid P component (SAP).
  • Long Pentraxins: Includes pentraxin 3 (PTX3), which has a longer N-terminal domain.
Biological Properties

Key Biological Properties:

  • Structure: Pentraxins have a cyclic pentameric structure, which is crucial for their function.
  • Expression Patterns: Short pentraxins like CRP are primarily produced in the liver, while long pentraxins like PTX3 are produced by a variety of cells including endothelial cells, macrophages, and dendritic cells.
  • Tissue Distribution: CRP is found in the blood plasma, whereas PTX3 is found in various tissues, especially at sites of inflammation.
Biological Functions

Primary Biological Functions:

  • Immune Responses: Pentraxins play a crucial role in the innate immune system by recognizing pathogens and damaged cells.
  • Pathogen Recognition: They bind to microbial pathogens and apoptotic cells, facilitating their clearance by immune cells.
Modes of Action

Mechanisms with Other Molecules and Cells:

  • Binding Partners: Pentraxins interact with complement proteins, Fc receptors, and other pattern recognition receptors.
  • Downstream Signaling Cascades: Upon binding to their targets, pentraxins activate complement pathways and enhance phagocytosis by immune cells.
Regulatory Mechanisms

Regulatory Mechanisms:

  • Transcriptional Regulation: The expression of pentraxins is regulated by cytokines such as IL-6 and IL-1β, which are released during inflammation.
  • Post-Translational Modifications: Glycosylation and phosphorylation are common modifications that affect the stability and function of pentraxins.
Applications

Biomedical Research:

  • Diagnostic Tools: CRP levels are commonly measured as a marker of inflammation and infection.
  • Therapeutic Strategies: PTX3 is being explored as a potential therapeutic target for inflammatory diseases and cancer.
Role in the Life Cycle

Role Throughout the Life Cycle:

  • Development: Pentraxins are involved in the early immune response during development.
  • Aging and Disease: Elevated levels of CRP are associated with aging and chronic inflammatory diseases such as cardiovascular disease and rheumatoid arthritis.
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