Recombinant Proteins

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TARDBP (1-414) Human, His

TAR DNA Binding Protein (1-414 a.a.) Human Recombinant, His Tag

Recombinant human TARDBP, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It encompasses 450 amino acids, including amino acids 1-414 of the TARDBP sequence, and has a molecular weight of 48.8 kDa. The protein includes a 36 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT10630
Source
E.coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

TARDBP Human

TAR DNA Binding Protein Human Recombinant

This product consists of a recombinant human TARDBP protein produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 296 amino acids (residues 1-260), with a molecular weight of 33.6 kDa. The protein includes a 36 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT10735
Source
E.coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

TARBP2 Human

TAR RNA Binding Protein 2 Human Recombinant

Recombinant human TARBP2 protein was expressed in E. coli and purified to a single, non-glycosylated polypeptide chain. This protein comprises amino acids 1-345 and possesses a molecular weight of 36.9 kDa.
Shipped with Ice Packs
Cat. No.
BT10430
Source
Escherichia Coli.
Appearance
Clear, colorless, and sterile-filtered solution.

TARDBP (1-414) Human

TAR DNA Binding Protein (1-414 a.a.) Human Recombinant

Recombinant human TARDBP, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 414 amino acids (1-414 a.a) with a molecular weight of 44.7 kDa. The protein is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT10551
Source
E.coli.
Appearance
Clear, colorless, and sterile-filtered solution.
Definition and Classification

TAR DNA refers to the trans-activation response (TAR) element, a sequence found in the long terminal repeat (LTR) of the human immunodeficiency virus type 1 (HIV-1) genome. This sequence forms a hairpin structure that is crucial for the transcriptional activation of the viral genome . TAR DNA-binding protein 43 (TDP-43) is a protein that binds to TAR DNA and is encoded by the TARDBP gene .

Biological Properties

Key Biological Properties: TDP-43 is a 414 amino acid protein with multiple domains, including an N-terminal domain, two RNA recognition motifs, and a C-terminal domain . It binds both DNA and RNA, playing roles in transcriptional repression, pre-mRNA splicing, and translational regulation .

Expression Patterns: TDP-43 is ubiquitously expressed in various tissues, with high levels in the brain and spinal cord .

Tissue Distribution: TDP-43 is predominantly localized in the nucleus but can shuttle between the nucleus and cytoplasm .

Biological Functions

Primary Biological Functions: TDP-43 is involved in multiple cellular processes, including transcriptional repression, RNA splicing, and mRNA stability . It binds to the TAR DNA sequence of HIV-1, repressing viral transcription .

Role in Immune Responses and Pathogen Recognition: TDP-43’s role in immune responses is primarily linked to its interaction with viral elements like TAR DNA, influencing the transcriptional activity of the virus .

Modes of Action

Mechanisms with Other Molecules and Cells: TDP-43 interacts with various RNA and DNA sequences, influencing their processing and stability . It forms complexes with other proteins, such as hnRNPs, to regulate RNA metabolism .

Binding Partners and Downstream Signaling Cascades: TDP-43 binds to UG-rich intronic regions of RNA, affecting splicing and stability . It also interacts with proteins involved in stress granule formation and autophagy .

Regulatory Mechanisms

Transcriptional Regulation: TDP-43 regulates its own expression through an autoregulatory feedback loop . It binds to its mRNA, influencing its stability and translation .

Post-Translational Modifications: TDP-43 undergoes various post-translational modifications, including phosphorylation, ubiquitination, and cleavage . These modifications affect its localization, stability, and function .

Applications

Biomedical Research: TDP-43 is a significant focus in neurodegenerative disease research, particularly in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD) .

Diagnostic Tools: TDP-43 inclusions are used as biomarkers for diagnosing ALS and FTLD .

Therapeutic Strategies: Targeting TDP-43 aggregation and mislocalization is a potential therapeutic strategy for neurodegenerative diseases .

Role in the Life Cycle

Development to Aging and Disease: TDP-43 is essential for normal development and cellular function . Its dysregulation is linked to various neurodegenerative diseases, highlighting its role in aging and disease progression .

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