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Recombinant Proteins
Product List
SRP54 Human
Signal Recognition Particle 54kDa Human Recombinant
This product consists of a full-length cDNA sequence encoding the human SRP54 protein. This protein has a molecular mass of 62kDa (determined at pH 8.9) and is fused to a hexa-histidine tag for purification purposes.
Shipped with Ice Packs 
SRP14 Human
Signal Recognition Particle 14kDa Human Recombinant
Recombinant human SRP14, with a 24 amino acid His tag attached to its N-terminus, is produced in E. coli. This single, non-glycosylated polypeptide chain consists of 160 amino acids (amino acids 1-136) and has a molecular weight of 17.1 kDa. Purification of SRP14 is achieved using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT26543
Source
Escherichia Coli.
Appearance
A clear, sterile solution without any color.
SRP19 Human
Signal Recognition Particle 19kDa Human Recombinant
SRP19 Human Recombinant is a genetically engineered protein produced in E. coli. It consists of a single, non-glycosylated polypeptide chain containing 167 amino acids (with amino acids 1-144 representing the SRP19 sequence) and a 23 amino acid His tag at the N-terminus. This recombinant protein has a molecular weight of 18.5 kDa and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT26645
Source
Escherichia Coli.
Appearance
Clear, colorless solution that has been sterilized through filtration.
Introduction
Definition and Classification
The Signal Recognition Particle (SRP) is a ribonucleoprotein complex that plays a critical role in the targeting and translocation of nascent proteins to the endoplasmic reticulum (ER) in eukaryotic cells or the plasma membrane in prokaryotic cells. SRP is classified based on its components and function, with the eukaryotic SRP being composed of a 7S RNA molecule and six protein subunits (SRP54, SRP19, SRP68, SRP72, SRP9, and SRP14).
Biological Properties
- Key Biological Properties: SRP is essential for the co-translational targeting of proteins to the ER. It recognizes and binds to signal sequences of nascent polypeptides emerging from the ribosome.
- Expression Patterns: SRP is ubiquitously expressed in all cells, given its fundamental role in protein targeting.
- Tissue Distribution: SRP is found in all tissues, with higher activity in cells with extensive secretory functions, such as liver and pancreas cells.
Biological Functions
- Primary Biological Functions: The primary function of SRP is to recognize signal peptides of nascent proteins and direct them to the ER for proper folding and modification.
- Role in Immune Responses: SRP indirectly supports immune responses by ensuring the proper synthesis and processing of proteins, including those involved in immune functions.
- Pathogen Recognition: While SRP itself does not directly recognize pathogens, its role in protein targeting is crucial for the synthesis of proteins that participate in pathogen recognition and immune defense.
Modes of Action
- Mechanisms with Other Molecules and Cells: SRP interacts with the ribosome, signal peptides, and the SRP receptor (SR) on the ER membrane.
- Binding Partners: Key binding partners include the ribosome, nascent polypeptide chains, and the SRP receptor.
- Downstream Signaling Cascades: Upon binding to the SRP receptor, SRP facilitates the transfer of the ribosome-nascent chain complex to the translocon, initiating protein translocation into the ER.
Regulatory Mechanisms
- Expression and Activity Control: SRP expression is regulated at the transcriptional level by various transcription factors.
- Transcriptional Regulation: Specific transcription factors and regulatory elements in the SRP gene promoter region control its expression.
- Post-Translational Modifications: SRP components may undergo post-translational modifications, such as phosphorylation, to modulate their activity and interactions.
Applications
- Biomedical Research: SRP is studied to understand protein targeting and translocation mechanisms.
- Diagnostic Tools: SRP components can be used as biomarkers for certain diseases involving protein misfolding and trafficking.
- Therapeutic Strategies: Targeting SRP pathways may offer therapeutic potential for diseases related to protein misfolding and secretion.
Role in the Life Cycle
- Development: SRP is crucial during development for the proper synthesis and targeting of proteins necessary for cell growth and differentiation.
- Aging: Changes in SRP function or expression may contribute to age-related declines in protein homeostasis.
- Disease: Dysregulation of SRP function can lead to diseases such as neurodegenerative disorders, where protein misfolding and aggregation are prominent features.
