Recombinant Proteins

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ICAM1 Human HEK

Intercellular Adhesion Molecule-1 Human Recombinant HEK

This product consists of the recombinant human ICAM1 protein, produced in a mammalian expression system using human cells. The protein encompasses amino acids 28 to 480 of the ICAM1 sequence, with an 8-amino acid His-tag added to the C-terminus. Purification is achieved through proprietary chromatographic techniques, resulting in a single polypeptide chain.
Shipped with Ice Packs
Cat. No.
BT25036
Source
HEK293 cells.
Appearance
The product appears as a sterile, white powder that has been lyophilized (freeze-dried).

ICAM1 Human, Sf9

Intercellular Adhesion Molecule-1 Human Recombinant, SF9

This product is a glycosylated recombinant human ICAM1 protein produced in Sf9 insect cells. It is a single polypeptide chain consisting of 692 amino acids, with a molecular weight of 76.5 kDa. The protein contains amino acids 28-480 of the mature ICAM1 sequence, a 239 amino acid His tag at the C-terminus, and undergoes purification using proprietary chromatographic techniques. On SDS-PAGE, it appears at approximately 70-100 kDa due to glycosylation.
Shipped with Ice Packs
Cat. No.
BT25144
Source
Sf9, Baculovirus cells.
Appearance
Clear, colorless, and sterile solution.

ICAM1 Mouse

Intercellular Adhesion Molecule-1 Mouse Recombinant

Recombinant Mouse ICAM1, expressed in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It comprises 466 amino acids (28-485 a.a), resulting in a molecular weight of 51.2kDa. The protein includes an 8 amino acid His-tag at the C-terminus and is purified using proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT25213
Source

Sf9, Baculovirus cells.

Appearance
A clear, colorless solution that has been sterilized by filtration.

CADM1 Human

Cell Adhesion Molecule 1 Human Recombinant

Recombinant human CADM1, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 353 amino acids (45-374 a.a.). It has a molecular mass of 39.4 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT24892
Source
Escherichia Coli.
Appearance
Sterile, colorless solution.

ICAM-1 Human

Intercellular Adhesion Molecule-1 Human Recombinant

This product consists of the recombinant human ICAM-1 protein, expressed in E. coli, with a hexahistidine tag at its amino terminus. The protein spans amino acids 28-483, totaling 455 amino acids. Purification is achieved through proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT24959
Source
Escherichia Coli.
Appearance
A clear solution that has been sterilized by filtration.

ICAM2 Human

Intercellular Adhesion Molecule-2 Human Recombinant

Recombinant human ICAM2, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 225 amino acids (residues 22-223). It has a molecular weight of 24.8 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT25308
Source
Escherichia Coli.
Appearance
A sterile, colorless solution.

ICAM3 Human

Intercellular Adhesion Molecule-3 Human Recombinant

Recombinant Human ICAM3, expressed in Sf9 insect cells using a baculovirus system, is a single, glycosylated polypeptide chain. This protein consists of 698 amino acids (residues 30-485), resulting in a molecular weight of 76.7kDa. For purification purposes, ICAM3 is fused to a 242 amino acid hIgG-His-Tag at the C-terminus and is purified using proprietary chromatographic methods.

Shipped with Ice Packs
Cat. No.
BT25426
Source

Sf9, Baculovirus cells.

Appearance
The product is a sterile, colorless, and clear solution.

ICAM5 Human

Intercellular Adhesion Molecule-5 Human Recombinant

Recombinant human ICAM5, produced in HEK293 cells, is a single, glycosylated polypeptide chain consisting of 1052 amino acids (23-835 a.a). It has a molecular mass of 113.8 kDa. The ICAM5 protein is fused to a 239 amino acid hIgG-His-tag at the C-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT25504
Source

HEK293.

Appearance
Sterile, colorless, and filtered solution.

L1CAM Human

L1 Cell Adhesion Molecule Human Recombinant

Recombinant human L1CAM, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It comprises 1104 amino acids (20-1115a.a.) and has a molecular mass of 123.6kDa. It's important to note that the molecular size on SDS-PAGE might appear between 100-150kDa. This L1CAM protein is expressed with an 8 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT25597
Source

Sf9, Baculovirus cells.

Appearance
A sterile, colorless solution.

NCAM1 Human

Neural Cell Adhesion Molecule 1 Human Recombinant

Recombinant human NCAM1, expressed in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It comprises 593 amino acids (20-603 a.a), resulting in a molecular weight of 65.7 kDa. This protein includes a 6-amino acid His-tag fused at the C-terminus and is purified using proprietary chromatographic methods.

Shipped with Ice Packs
Cat. No.
BT25664
Source

Sf9, Baculovirus cells.

Appearance

Sterile filtered, colorless solution.

Definition and Classification

Intercellular Adhesion Molecules (ICAMs) are a group of cell surface glycoproteins that play a crucial role in the immune response by facilitating the adhesion and interaction of leukocytes with endothelial cells. They belong to the immunoglobulin superfamily and are classified into several types, including ICAM-1, ICAM-2, ICAM-3, ICAM-4, and ICAM-5, each with distinct structural and functional properties.

Biological Properties

Key Biological Properties: ICAMs are characterized by their ability to mediate cell-cell adhesion, which is essential for immune surveillance and response. They possess immunoglobulin-like domains that facilitate binding to integrins on leukocytes.

Expression Patterns: ICAMs are expressed on various cell types, including endothelial cells, epithelial cells, and immune cells. Their expression can be upregulated in response to inflammatory cytokines such as TNF-α and IL-1β.

Tissue Distribution: ICAMs are widely distributed across different tissues, with high expression levels in the vascular endothelium, lymphoid organs, and sites of inflammation.

Biological Functions

Primary Biological Functions: The primary function of ICAMs is to mediate the adhesion and transmigration of leukocytes across the endothelium during immune responses. They also play a role in antigen presentation and the activation of T cells.

Role in Immune Responses: ICAMs are critical for the recruitment of immune cells to sites of infection or injury. They facilitate the firm adhesion of leukocytes to the endothelium, allowing them to migrate into tissues and mount an effective immune response.

Pathogen Recognition: Some pathogens, such as rhinoviruses, exploit ICAMs as receptors to gain entry into host cells, highlighting their role in pathogen recognition and infection.

Modes of Action

Mechanisms with Other Molecules and Cells: ICAMs interact with integrins, such as LFA-1 and Mac-1, on the surface of leukocytes. This interaction is crucial for the firm adhesion and transmigration of leukocytes.

Binding Partners: ICAMs bind to integrins on leukocytes, facilitating cell-cell adhesion. They also interact with other adhesion molecules and signaling receptors on endothelial and immune cells.

Downstream Signaling Cascades: The binding of ICAMs to integrins triggers intracellular signaling cascades that promote cytoskeletal rearrangements, cell migration, and the activation of immune responses.

Regulatory Mechanisms

Regulatory Mechanisms: The expression and activity of ICAMs are tightly regulated at multiple levels, including transcriptional and post-transcriptional mechanisms.

Transcriptional Regulation: The transcription of ICAM genes is regulated by various transcription factors, such as NF-κB and AP-1, which are activated in response to inflammatory stimuli.

Post-Translational Modifications: ICAMs undergo post-translational modifications, such as glycosylation, which are essential for their proper folding, stability, and function.

Applications

Biomedical Research: ICAMs are widely studied in biomedical research for their role in immune responses and inflammation. They serve as valuable models for understanding cell adhesion and signaling mechanisms.

Diagnostic Tools: Soluble forms of ICAMs (sICAMs) are used as biomarkers for various inflammatory and autoimmune diseases, such as rheumatoid arthritis and cardiovascular diseases.

Therapeutic Strategies: Targeting ICAMs with monoclonal antibodies or small molecules is being explored as a therapeutic strategy for treating inflammatory diseases, cancer, and viral infections.

Role in the Life Cycle

Development: ICAMs play a role in embryonic development by mediating cell-cell interactions and tissue formation.

Aging: The expression and function of ICAMs can be altered with aging, contributing to age-related changes in immune function and inflammation.

Disease: Dysregulation of ICAM expression and function is associated with various diseases, including chronic inflammatory conditions, autoimmune diseases, and cancer.

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