Recombinant Proteins

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Product List

EPHA2 Human

EPH Receptor A2 Human Recombinant

EPHA2 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (Ala24-Glu530) containing a total of 515 amino acids, having a calculated molecular mass of 56.9kDa. The EPHA2 protein is fused to a 2 aa C-terminal linker and a 6 aa C-terminal His tag.
Shipped with Ice Packs
Cat. No.
BT1964
Source
HEK 293.
Appearance
Filtered White lyophilized (freeze-dried) powder.

EPHA2 Human, sf9

EPH Receptor A2 Human Recombinant, sf9

EPHA2 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 520 amino acids (27-537) and having a molecular mass of 57.3kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
EPHA2 is fused to 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT2021
Source
Sf9, Baculovirus cells.
Appearance
Sterile Filtered clear solution.

EPHA3 Mouse

EPH Receptor A3 Mouse Recombinant

EPHA3 Mouse Recombinant produced in HEK293 cells is a single, polypeptide chain containing 527 amino acids (21-541 a.a.) and having a molecular mass of 59.5kDa. EPHA3 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT2064
Source

HEK293 Cells.

Appearance
Sterile Filtered colorless solution.

EPHA4 Mouse

EPH Receptor A4 Mouse Recombinant

EPHA4 Mouse Recombinant produced in Sf9 Baculovirus cells is a single polypeptide chain containing 536 amino acids (20-547) and having a molecular mass of 59.3kDa.
(Molecular size on SDS-PAGE will appear at approximately 50-70KDa).
EPHA4 is fused to 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT2184
Source
Sf9, Baculovirus cells.
Appearance
Sterile Filtered clear solution.

EPHB1 Human

EPH Receptor B1 Human Recombinant

EPHB1 Human Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 529 amino acids (18-540 a.a) and having a molecular mass of 59.2 kDa.
EPHB1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT2231
Source

HEK293 Cells.

Appearance

Sterile filtered colorless solution.

EPHB2 Human

EPH Receptor B2 Human Recombinant

EPHB2 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 533 amino acids (19-543a.a) and having a molecular mass of 59.1kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). EPHB2 is fused to a 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT2297
Source
Sf9, Baculovirus cells.
Appearance
Sterile Filtered colorless solution.

EPHB4 Mouse

EPH Receptor B4 Mouse Recombinant

EPHB4 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 532 amino acids (16-539 a.a.) and having a molecular mass of 58.7kDa (Migrates at 50-70kDa on SDS-PAGE under reducing conditions).
EPHB4 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT2395
Source
Sf9, Baculovirus cells.
Appearance
Sterile Filtered clear solution.

Introduction

Definition and Classification

Eph receptors, named after their expression in an erythropoietin-producing human hepatocellular carcinoma cell line, represent the largest family of receptor tyrosine kinases (RTKs) in the animal kingdom . They are divided into two subclasses, EphA and EphB, based on sequence similarity and their binding affinity for either the glycosylphosphatidylinositol (GPI)-linked ephrin-A ligands or the transmembrane-bound ephrin-B ligands . Humans express nine EphA receptors (EphA1-8 and EphA10) and five EphB receptors (EphB1-4 and EphB6) .

Biological Properties

Eph receptors are widely expressed in various tissues and play crucial roles in numerous biological processes. They are involved in cell positioning, tissue patterning, and organ development . Eph receptors are expressed in most, if not all, cell types and are essential for cell contact-dependent communication . Their expression patterns and tissue distribution are highly regulated, with specific receptors being predominant in certain tissues .

Biological Functions

Eph receptors are pivotal in regulating cell migration, adhesion, differentiation, and proliferation . They play significant roles in the development of the nervous system, cardiovascular system, and other organs . Eph/ephrin signaling is crucial for immune responses and pathogen recognition, influencing cell-cell communication and immune cell migration .

Modes of Action

Eph receptors interact with their ligands, ephrins, on neighboring cells, leading to bidirectional signaling . This interaction can result in either cell-cell adhesion or repulsion, depending on the relative expression and affinity of the ligand-receptor pairs . The signaling cascades initiated by Eph/ephrin interactions involve autophosphorylation of tyrosine residues and recruitment of downstream signaling molecules .

Regulatory Mechanisms

The expression and activity of Eph receptors are tightly regulated at multiple levels. Transcriptional regulation, post-translational modifications, and interactions with other signaling pathways all contribute to the precise control of Eph receptor functions . Proteolytic cleavage and alternative splicing further increase the diversity and complexity of Eph receptor signaling .

Applications

Eph receptors have significant applications in biomedical research, diagnostic tools, and therapeutic strategies. They are potential targets for cancer therapy due to their roles in tumor progression, angiogenesis, and metastasis . Eph receptors are also being explored as targets for immunotherapy and as biomarkers for various diseases .

Role in the Life Cycle

Eph receptors are involved in critical processes throughout the life cycle, from embryonic development to aging . During development, they regulate cell sorting, migration, and boundary formation . In adulthood, Eph receptors maintain tissue homeostasis and are implicated in neural plasticity and immune responses . Dysregulation of Eph signaling is associated with various diseases, including cancer and neurological disorders .

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