Recombinant Proteins

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FZD10 Human

Frizzled Class Receptor 10 Human Recombinant

Recombinant human FZD10 is a glycosylated polypeptide chain encompassing amino acids 21-225, resulting in a 444-amino acid protein with a molecular weight of 50.0 kDa. This protein is further modified by the addition of a 239-amino acid hIgG-His-Tag at the C-terminus. Purification is achieved through proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT20002
Source

HEK293 Cells.

Appearance
Sterile Filtered colorless solution.

FZD4 Human

Frizzled Class Receptor 4 Human Recombinant

FZD4 Human Recombinant, produced in Sf9 Insect cells, is a single, glycosylated polypeptide chain containing 428 amino acids (37-222 a.a.). It has a molecular mass of 48.3kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). FZD4 is expressed with a 239 amino acids hIgG-His tag at the C-Terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20077
Source
Sf9, Baculovirus cells.
Appearance
Sterile Filtered colorless solution.

SFRP2 Human

Secreted Frizzled-Related Protein 2 Human Recombinant

Recombinant human SFRP2, expressed in E. coli, is a non-glycosylated polypeptide chain. This single-chain protein consists of 297 amino acids, with a sequence spanning from amino acid positions 20 to 295. A 21 amino acid His-tag is fused to the N-terminus to facilitate purification. The protein has a molecular weight of 33.8 kDa and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20274
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterile-filtered.

SFRP2 Mouse

Secreted Frizzled-Related Protein 2 Mouse Recombinant

Recombinant mouse SFRP2, expressed in Sf9 insect cells using a baculovirus expression system, is a single polypeptide chain with glycosylation. This protein comprises 280 amino acids (spanning from amino acid positions 25 to 295) and exhibits a molecular weight of 32.1 kDa. For purification purposes, a 6-amino acid Histidine tag has been incorporated at the C-terminus of the SFRP2 protein. The purification process involves proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20375
Source

Sf9, Baculovirus cells.

Appearance
Clear, colorless solution that has undergone sterile filtration.

SFRP4 Human, sf9

Secreted Frizzled-Related Protein 4 Human Recombinant, sf9

SFRP4, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain with a molecular weight of 38.9kDa. The protein sequence consists of 337 amino acids (19-346 a.a.) and on SDS-PAGE, it appears at approximately 40-57kDa. This discrepancy in size is attributed to glycosylation. The SFRP4 protein is expressed with a 9 amino acid His tag at its C-Terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20439
Source
Sf9, Baculovirus cells.
Appearance
A clear, colorless solution that has been sterilized by filtration.

SFRP4 Human

Secreted Frizzled-Related Protein 4 Human Recombinant

Recombinant Human SFRP4, produced in HEK cells, is a single, glycosylated polypeptide chain encompassing amino acids 19-346. This totals 341 amino acids, resulting in a calculated molecular mass of 39kDa. However, it exhibits a migration pattern corresponding to approximately 55kDa on SDS-PAGE. This discrepancy is attributed to glycosylation. The SFRP4 construct includes a 5 amino acid N-terminal linker and an 8 amino acid Flag tag at the N-terminus. Purification of Human SFRP4 is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20545
Source
HEK 293.
Appearance
White lyophilized powder after filtration.

SFRP5 Human

Secreted Frizzled-Related Protein 5 Human Recombinant

Recombinant human SFRP5, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 311 amino acids (30-317 a.a.). It has a molecular weight of 35 kDa. The SFRP5 protein is fused to a 23 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20611
Source
Escherichia Coli.
Appearance
Clear, sterile-filtered solution.
Definition and Classification

Secreted Frizzled-Related Proteins (SFRPs) are a family of secreted glycoproteins that act as extracellular signaling ligands. They are structurally related to Frizzled (Fz) receptors, sharing a cysteine-rich domain (CRD) that enables them to bind to Wnt ligands or Fz receptors . The SFRP family consists of five members: SFRP1, SFRP2, SFRP3, SFRP4, and SFRP5 .

Biological Properties

Key Biological Properties: SFRPs are known for their ability to bind Wnt proteins and Fz receptors, thereby modulating Wnt signaling pathways . They can act as antagonists to Wnt signaling by preventing Wnt proteins from binding to Fz receptors .

Expression Patterns and Tissue Distribution: SFRPs are expressed in various tissues and have distinct expression patterns. For example, SFRP1 is expressed in the retina, SFRP2 in the heart and vasculature, SFRP3 in the brain, SFRP4 in the pancreas, and SFRP5 in adipose tissue .

Biological Functions

Primary Biological Functions: SFRPs play crucial roles in regulating cell growth, differentiation, and tissue homeostasis . They are involved in processes such as embryonic development, bone formation, and angiogenesis .

Role in Immune Responses and Pathogen Recognition: SFRPs have been implicated in modulating immune responses by influencing the activity of immune cells and cytokine production . They can also play a role in pathogen recognition by interacting with receptors on immune cells .

Modes of Action

Mechanisms with Other Molecules and Cells: SFRPs interact with Wnt proteins and Fz receptors in the extracellular compartment, forming inhibitory complexes that prevent Wnt signaling . They can also bind to other receptors and matrix molecules, influencing various signaling pathways .

Binding Partners and Downstream Signaling Cascades: SFRPs bind to Wnt ligands and Fz receptors, modulating downstream signaling cascades such as the canonical Wnt/β-catenin pathway and noncanonical Wnt/Ca2+ pathway . These interactions can lead to changes in gene transcription and cell behavior .

Regulatory Mechanisms

Transcriptional Regulation: The expression of SFRPs is regulated at the transcriptional level by various transcription factors and signaling pathways . For example, Wnt signaling itself can regulate the expression of SFRPs through feedback mechanisms .

Post-Translational Modifications: SFRPs undergo post-translational modifications such as glycosylation, which can affect their stability, secretion, and interactions with other molecules .

Applications

Biomedical Research: SFRPs are used as tools to study Wnt signaling pathways and their roles in development and disease . They are also investigated as potential biomarkers for various diseases, including cancer and metabolic disorders .

Diagnostic Tools and Therapeutic Strategies: SFRPs have potential applications in diagnostics and therapeutics. For example, SFRP2 is being explored as a target for anti-angiogenic therapies in cancer . SFRP4 has been identified as a biomarker for β-cell dysfunction and insulin resistance in diabetes .

Role in the Life Cycle

Development: SFRPs are essential for proper embryonic development, influencing processes such as cell differentiation, tissue patterning, and organogenesis .

Aging and Disease: The expression and activity of SFRPs can change with aging, contributing to age-related diseases such as cancer, osteoporosis, and neurodegenerative disorders . Dysregulation of SFRP expression is also associated with various pathologies, including cardiovascular diseases and metabolic disorders .

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