Recombinant Proteins

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SLAMF6 Human, sf9

SLAMF6 Human Recombinant, sf9

Recombinant human SLAMF6, produced in insect cells, is a single glycosylated polypeptide chain comprising 214 amino acids (residues 22-226). It has a molecular weight of 24.1 kDa, but on SDS-PAGE, it appears around 28-40 kDa due to glycosylation. The protein contains a C-terminal 6-amino acid His-tag and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT28645
Source
Baculovirus sf9 cells.
Appearance
Clear, sterile-filtered solution.

SLAMF7 Human

SLAMF7 Human Recombinant

Produced in Sf9 Baculovirus cells, SLAMF7 is a single, glycosylated polypeptide chain with a molecular weight of 23.4kDa (212 amino acids, spanning from 23-226a.a.). Despite its actual size, it appears between 28-40kDa on SDS-PAGE due to glycosylation. This protein is engineered with an 8 amino acid His tag at the C-terminus and undergoes purification using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT28722
Source
Sf9, Baculovirus cells.
Appearance
A clear solution that has been sterilized by filtration.

SLAMF1 Human

SLAMF1 Human Recombinant

Recombinant human SLAMF1, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 240 amino acids (residues 21-237). It has a molecular weight of 26.7 kDa. The protein consists of the SLAMF1 sequence with an N-terminal 23 amino acid His-tag. Purification is achieved using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT28378
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

SLAMF1 Human, Sf9

SLAMF1 Human Recombinant, Sf9

Recombinant human SLAMF1, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain consisting of 226 amino acids (21-237a.a.). It has a molecular mass of 25.3 kDa. Note: On SDS-PAGE, the molecular size will appear between 28-40 kDa. This SLAMF1 protein is expressed with a 6 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT28446
Source

Sf9, Baculovirus cells.

Appearance
Clear, sterile solution.

SLAMF6 Human

SLAMF6 Human Recombinant

Recombinant SLAMF6, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 228 amino acids (22-226 a.a.). With a molecular weight of 25.5 kDa, it consists of the SLAMF6 protein fused to a 23 amino acid His-tag at the N-terminus. The protein is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT28527
Source
Escherichia Coli.
Appearance
Clear solution, sterile-filtered.
Definition and Classification

The Signaling Lymphocytic Activation Molecule (SLAM) family is a group of cell surface receptors belonging to the immunoglobulin superfamily. These receptors are primarily involved in the regulation of immune responses. The SLAM family consists of nine members: SLAMF1 (CD150), SLAMF2 (CD48), SLAMF3 (CD229), SLAMF4 (CD244), SLAMF5 (CD84), SLAMF6 (CD352), SLAMF7 (CD319), SLAMF8 (CD353), and SLAMF9 .

Biological Properties

Key Biological Properties: SLAM family receptors are type I transmembrane glycoproteins characterized by an amino-terminal IgV variable domain and a membrane-adjacent IgC2 constant domain. They also contain immunoreceptor tyrosine-based switch motifs (ITSMs) in their cytoplasmic domains .

Expression Patterns and Tissue Distribution: SLAM receptors are expressed on a wide range of immune cells, including B and T cells, natural killer (NK) cells, dendritic cells, macrophages, eosinophils, neutrophils, and platelets. The expression patterns vary among different SLAM family members .

Biological Functions

Primary Biological Functions: SLAM family receptors play crucial roles in immune cell communication and activation. They enhance T cell proliferation by stimulating the production of cytokines such as IL-4 and IFN-gamma. SLAM receptors also facilitate cell-to-cell adhesion during antigen presentation .

Role in Immune Responses and Pathogen Recognition: SLAM receptors can interact directly with microbes, promoting phagocytic cell migration to infection sites. For example, SLAMF1 is known to enhance phagocytosis by localizing to phagosomes and inducing signaling cascades that result in the fusion of phagosomes and lysosomes .

Modes of Action

Mechanisms with Other Molecules and Cells: SLAM receptors typically engage in homophilic interactions, meaning they bind to identical receptors on adjacent cells. This binding triggers downstream signaling cascades involving SLAM-associated proteins (SAPs) and other adaptor molecules .

Binding Partners and Downstream Signaling Cascades: SLAM receptors recruit SAPs, which in turn recruit Src family kinases like Fyn. This leads to the phosphorylation of ITSMs and the activation of downstream signaling pathways that promote immune cell activation and cytokine production .

Regulatory Mechanisms

Transcriptional Regulation: The expression of SLAM family receptors is regulated at the transcriptional level by various cytokines and transcription factors. For instance, the differentiation of monocytes into macrophages can upregulate SLAMF7 expression .

Post-Translational Modifications: SLAM receptors undergo post-translational modifications such as phosphorylation, which is crucial for their signaling functions. Phosphorylation of ITSMs is necessary for the binding of SAPs and the subsequent activation of downstream signaling pathways .

Applications

Biomedical Research: SLAM family receptors are valuable tools in immunological research due to their roles in immune cell activation and communication. They are studied in the context of various diseases, including immunodeficiencies and autoimmune disorders .

Diagnostic Tools and Therapeutic Strategies: SLAM receptors are potential targets for diagnostic and therapeutic applications. For example, monoclonal antibodies targeting SLAMF7 are being developed for the treatment of multiple myeloma .

Role in the Life Cycle

Development to Aging and Disease: SLAM family receptors are involved in various stages of the immune response throughout the life cycle. They play roles in the development of immune cells, their activation during infections, and the regulation of immune responses in aging and disease .

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