Recombinant Proteins

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AHSG

Alpha-2-HS-Glycoprotein Human

Human Alpha-2-HS-Glycoprotein, comprising 349 amino acids, was purified from human plasma. The calculated molecular mass, excluding glycosylation, is 37.3 kDa.
Shipped with Ice Packs
Cat. No.
BT18617
Source
Purified from the human plasma.
Appearance
White lyophilized powder that has undergone filtration.

AHSG Human

Alpha-2-HS-Glycoprotein Human Recombinant

Recombinant human AHSG, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 372 amino acids (residues 19-367). This protein variant has a molecular weight of 39.7 kDa, although it may appear larger on SDS-PAGE due to the presence of a 23 amino acid His-tag at the N-terminus. The purification process involves proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT18686
Source
Escherichia Coli.
Appearance
The product is a sterile, colorless solution.

AHSG Human HEK

Alpha-2-HS-Glycoprotein Human Recombinant HEK

Recombinant human AHSG, produced in transfected human cells, is a single polypeptide chain consisting of 357 amino acids (residues 19-367). An 8 amino acid His-tag is fused to the C-terminus of AHSG, and the protein is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT18753
Source
HEK293 cells.
Appearance
Sterile Filtered White lyophilized powder.

AHSG Mouse

Alpha-2-HS-Glycoprotein Mouse Recombinant

Recombinant AHSG protein, expressed in Sf9 insect cells using a baculovirus system, is a single polypeptide chain containing 333 amino acids (residues 19-345). This glycosylated protein has a molecular weight of 36.1 kDa, although it may appear between 40-57 kDa on SDS-PAGE due to glycosylation. The recombinant AHSG protein includes a 6-amino acid Histidine tag at the C-terminus to facilitate purification via proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT18836
Source
Sf9, Baculovirus cells.
Appearance
A clear, colorless solution that has been sterilized by filtration.
Definition and Classification

Alpha-2-HS-glycoprotein (AHSG), also known as fetuin-A, is a plasma glycoprotein encoded by the AHSG gene . It belongs to the fetuin class of plasma binding proteins and is more abundant in fetal blood than in adult blood . AHSG is synthesized primarily by hepatocytes and adipocytes .

Biological Properties

Key Biological Properties: AHSG is a negatively charged serum glycoprotein that consists of two polypeptide chains cleaved from a single mRNA . It has a molecular weight of approximately 49,000 Daltons .

Expression Patterns: AHSG is predominantly expressed in the liver and adipose tissues . It is also present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix .

Tissue Distribution: AHSG is found in various tissues, including the liver, gallbladder, fetal liver, hematopoietic progenitor cells, and the choroid plexus .

Biological Functions

Primary Biological Functions: AHSG plays a role in endocytosis, brain development, and bone tissue formation . It also has opsonic properties and influences the mineral phase of bone .

Role in Immune Responses and Pathogen Recognition: AHSG is involved in the regulation of inflammatory responses and has been shown to negatively regulate the insulin receptor signaling pathway . It also plays a role in the acute-phase response and neutrophil degranulation .

Modes of Action

Mechanisms with Other Molecules and Cells: AHSG interacts with various molecules and cells through its binding partners. It shows affinity for calcium and barium ions, which may influence its role in bone mineralization .

Binding Partners: AHSG can bind multiple ligands, including endopeptidase inhibitors and kinases . It has been identified as a key glycoprotein in exerting cytoprotective activity against oxidative injury in neuronal cells .

Downstream Signaling Cascades: AHSG is involved in several signaling pathways, including the regulation of insulin-like growth factor transport and uptake by insulin-like growth factor binding proteins .

Regulatory Mechanisms

Transcriptional Regulation: The expression of AHSG is regulated by various factors, including testosterone via androgen receptor activation . The AHSG gene harbors putative androgen receptor response elements in its promoter sequence .

Post-Translational Modifications: AHSG undergoes complex proteolytic processing and circulates in blood and extracellular fluids as a partially phosphorylated glycoprotein .

Applications

Biomedical Research: AHSG is used in research to study its role in bone mineralization, inflammation, and metabolic processes .

Diagnostic Tools: AHSG levels are measured as biomarkers for various conditions, including cardiovascular diseases and metabolic disorders .

Therapeutic Strategies: AHSG has potential therapeutic applications in neuroprotection and the treatment of neurological diseases .

Role in the Life Cycle

Development: AHSG is more abundant in fetal blood and plays a crucial role in brain development and bone tissue formation during early development .

Aging and Disease: AHSG levels and activity are associated with various age-related conditions, including cardiovascular diseases and metabolic disorders . It also contributes to the prevention of soft tissue calcification and modulates inflammation .

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