Recombinant Proteins

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OmpA

Outer Membrane Protein-A Bacterial Recombinant

The recombinant A-protein was found to be very similar to the naturally occurring (wild type) form. Both SDS-PAGE and gel filtration chromatography showed it to be a 50.5 kDa monomer. Immunological techniques, including ELISA and Western Blot using polyclonal and monoclonal antibodies, further confirmed the similarity between the recombinant and wild type A-proteins. Significantly, all forms of A-protein were able to activate the release of tumor necrosis factor alpha from murine macrophages. For further details, refer to Maurice et al. (1999) Protein Expression and Purification 16, 396-404. The purification of OmpA is carried out using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT2392
Source
Escherichia Coli.
Appearance
Sterile Filtered White lyophilized powder
Definition and Classification

Outer Membrane Proteins (OMPs) are integral membrane proteins located in the outer membrane of Gram-negative bacteria, mitochondria, and chloroplasts. They play crucial roles in various cellular processes. OMPs can be classified into several categories based on their structure and function:

  • Porins: Form channels that allow the passive diffusion of small molecules.
  • Transporters: Facilitate the active transport of specific substrates.
  • Enzymes: Catalyze biochemical reactions.
  • Receptors: Involved in signal transduction and communication with the environment.
Biological Properties

Key Biological Properties:

  • Structure: Typically β-barrel structures that span the membrane.
  • Stability: Highly stable due to their β-barrel configuration.
  • Hydrophobicity: Hydrophobic exterior interacts with the lipid bilayer, while the interior is often hydrophilic.

Expression Patterns:

  • OMPs are expressed in a regulated manner, often in response to environmental conditions.

Tissue Distribution:

  • Found in the outer membrane of Gram-negative bacteria, mitochondria, and chloroplasts.
Biological Functions

Primary Biological Functions:

  • Nutrient Uptake: Facilitate the uptake of essential nutrients.
  • Waste Removal: Help in the expulsion of waste products.
  • Structural Integrity: Contribute to the structural integrity of the outer membrane.

Role in Immune Responses:

  • Pathogen Recognition: Recognized by the host immune system, triggering immune responses.
  • Antigen Presentation: Some OMPs act as antigens, eliciting an immune response.
Modes of Action

Mechanisms with Other Molecules and Cells:

  • Binding Partners: Interact with various molecules such as lipopolysaccharides, peptidoglycans, and other proteins.
  • Downstream Signaling Cascades: Trigger signaling pathways that lead to cellular responses.
Regulatory Mechanisms

Control of Expression and Activity:

  • Transcriptional Regulation: Controlled by transcription factors and environmental signals.
  • Post-Translational Modifications: Modifications such as phosphorylation and glycosylation can affect their function and stability.
Applications

Biomedical Research:

  • Model Systems: Used as models to study membrane protein structure and function.
  • Drug Targets: Potential targets for antibiotics and other therapeutic agents.

Diagnostic Tools:

  • Biomarkers: Serve as biomarkers for the detection of bacterial infections.

Therapeutic Strategies:

  • Vaccine Development: Used in the development of vaccines against bacterial pathogens.
Role in the Life Cycle

Throughout the Life Cycle:

  • Development: Essential for the proper development and functioning of bacterial cells.
  • Aging: Changes in OMP expression and function can occur with aging.
  • Disease: Alterations in OMPs are associated with various diseases, including bacterial infections and mitochondrial disorders.
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