Recombinant Proteins

p53
LBP
CEA
HLA
TCL
TTC
NPM
MAF
Bax
BID

GABARAP Human

GABA(A) Receptor-Associated Protein Human Recombinant

Recombinant human GABARAP, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 137 amino acids (specifically, amino acids 1 to 117). It possesses a molecular mass of 16 kDa. A 20 amino acid His-Tag is fused to the N-terminus of GABARAP. Purification is achieved using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT10065
Source
Escherichia Coli.
Appearance
A clear and colorless solution that has undergone sterile filtration.

GABARAPL1 Human

GABA(A) Receptor-Associated Protein Like 1 Human Recombinant

GABARAPL1 Human Recombinant is a laboratory-produced protein. It is made in E. coli bacteria and contains a His tag to help with purification. This protein is a single chain of 137 amino acids, including a 20 amino acid His tag at the beginning. It has a molecular weight of 16.2kDa but may appear larger on SDS-PAGE. The protein is purified using specific chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT10135
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

GABARAPL2 Human

GABA(A) Receptor-Associated Protein Like 2 Human Recombinant

GABARAPL2, produced in E. coli, is a single polypeptide chain that lacks glycosylation. It comprises 137 amino acids (specifically, amino acids 1 through 117) and has a molecular weight of 15.8 kDa. The protein is engineered with a 20-amino acid His-tag fused to its N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT10182
Source
Escherichia Coli.
Appearance
A clear solution that has undergone sterile filtration.
Definition and Classification

Gamma-aminobutyric acid A receptor-associated protein (GABARAP) is a protein encoded by the GABARAP gene in humans. It shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B . GABARAP is involved in clustering neurotransmitter receptors by mediating interaction with the cytoskeleton .

Biological Properties

Key Biological Properties: GABARAP is highly positively charged in its N-terminus . It is a member of the microtubule-associated protein family and plays a role in intracellular receptor transport and autophagy pathways .

Expression Patterns and Tissue Distribution: GABARAP is expressed in various tissues, including the central nervous system, where it clusters neurotransmitter receptors . It is also involved in selective autophagy processes, including mitochondrial autophagy, endoplasmic reticulum autophagy, and Golgi autophagy .

Biological Functions

Primary Biological Functions: GABARAP clusters neurotransmitter receptors by mediating interaction with the cytoskeleton . It is involved in intracellular receptor transport and autophagy pathways .

Role in Immune Responses and Pathogen Recognition: GABARAP plays a role in the immune response by participating in selective autophagy processes, which are crucial for pathogen recognition and elimination .

Modes of Action

Mechanisms with Other Molecules and Cells: GABARAP interacts with the cytoskeleton to cluster neurotransmitter receptors . It also interacts with various proteins involved in autophagy pathways .

Binding Partners and Downstream Signaling Cascades: GABARAP binds to microtubule-associated proteins and other autophagy-related proteins to facilitate intracellular receptor transport and autophagy .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: GABARAP expression and activity are regulated by transcriptional and post-translational modifications . Protein transport, local protein synthesis, and synaptic protein degradation shape the transmission efficiency of excitatory synapses .

Transcriptional Regulation and Post-Translational Modifications: Calcium entering through L-type voltage-gated calcium channels can regulate GABARAP function by means of changes in transcription, phosphorylation, trafficking, and lateral diffusion of receptors on the plasma membrane .

Applications

Biomedical Research: GABARAP is a target for studying intracellular receptor transport and autophagy pathways .

Diagnostic Tools: Fluorescent peptides designed from GABARAP binders enable live neuronal staining and visualization of gephyrin in the postsynaptic sites with submicron resolution .

Therapeutic Strategies: Targeting GABARAP and its associated proteins can modulate GABAergic signaling, providing potential therapeutic strategies for neurodevelopmental disorders, neurodegenerative diseases, and mental illnesses .

Role in the Life Cycle

Role Throughout the Life Cycle: GABARAP is involved in various life processes, including intracellular receptor transport and autophagy pathways . It plays a crucial role in the regulation of many life processes, such as lowering blood pressure, treating anxiety and arrhythmia, controlling hormone secretion, and enhancing liver and kidney functions .

GABARAP’s involvement in these processes highlights its importance from development to aging and disease .

© Copyright 2024 Thebiotek. All Rights Reserved.