Recombinant Proteins

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Product List

GABARAP Human

GABA(A) Receptor-Associated Protein Human Recombinant

GABARAP Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 137 amino acids (1-117 a.a.) and having a molecular mass of 16 kDa. GABARAP is fused to a 20 amino acid His-Tag at N-Terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT10065
Source
Escherichia Coli.
Appearance
Sterile filtered colorless solution.

GABARAPL1 Human

GABA(A) Receptor-Associated Protein Like 1 Human Recombinant

GABARAPL1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 137 amino acids (1-117 a.a.) and having a molecular mass of 16.2kDa (Molecular size on SDS-PAGE will appear higher). The GABARAPL1 is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT10135
Source
Escherichia Coli.
Appearance
Sterile Filtered colorless solution.

GABARAPL2 Human

GABA(A) Receptor-Associated Protein Like 2 Human Recombinant

GABARAPL2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 137 amino acids (1-117a.a.) and having a molecular mass of 15.8kDa.
GABARAPL2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT10182
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

Introduction

Definition and Classification

Gamma-aminobutyric acid A receptor-associated protein (GABARAP) is a protein encoded by the GABARAP gene in humans. It shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B . GABARAP is involved in clustering neurotransmitter receptors by mediating interaction with the cytoskeleton .

Biological Properties

Key Biological Properties: GABARAP is highly positively charged in its N-terminus . It is a member of the microtubule-associated protein family and plays a role in intracellular receptor transport and autophagy pathways .

Expression Patterns and Tissue Distribution: GABARAP is expressed in various tissues, including the central nervous system, where it clusters neurotransmitter receptors . It is also involved in selective autophagy processes, including mitochondrial autophagy, endoplasmic reticulum autophagy, and Golgi autophagy .

Biological Functions

Primary Biological Functions: GABARAP clusters neurotransmitter receptors by mediating interaction with the cytoskeleton . It is involved in intracellular receptor transport and autophagy pathways .

Role in Immune Responses and Pathogen Recognition: GABARAP plays a role in the immune response by participating in selective autophagy processes, which are crucial for pathogen recognition and elimination .

Modes of Action

Mechanisms with Other Molecules and Cells: GABARAP interacts with the cytoskeleton to cluster neurotransmitter receptors . It also interacts with various proteins involved in autophagy pathways .

Binding Partners and Downstream Signaling Cascades: GABARAP binds to microtubule-associated proteins and other autophagy-related proteins to facilitate intracellular receptor transport and autophagy .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: GABARAP expression and activity are regulated by transcriptional and post-translational modifications . Protein transport, local protein synthesis, and synaptic protein degradation shape the transmission efficiency of excitatory synapses .

Transcriptional Regulation and Post-Translational Modifications: Calcium entering through L-type voltage-gated calcium channels can regulate GABARAP function by means of changes in transcription, phosphorylation, trafficking, and lateral diffusion of receptors on the plasma membrane .

Applications

Biomedical Research: GABARAP is a target for studying intracellular receptor transport and autophagy pathways .

Diagnostic Tools: Fluorescent peptides designed from GABARAP binders enable live neuronal staining and visualization of gephyrin in the postsynaptic sites with submicron resolution .

Therapeutic Strategies: Targeting GABARAP and its associated proteins can modulate GABAergic signaling, providing potential therapeutic strategies for neurodevelopmental disorders, neurodegenerative diseases, and mental illnesses .

Role in the Life Cycle

Role Throughout the Life Cycle: GABARAP is involved in various life processes, including intracellular receptor transport and autophagy pathways . It plays a crucial role in the regulation of many life processes, such as lowering blood pressure, treating anxiety and arrhythmia, controlling hormone secretion, and enhancing liver and kidney functions .

GABARAP’s involvement in these processes highlights its importance from development to aging and disease .

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