Recombinant Proteins

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RAET1G Human

Retinoic Acid Early Transcript 1G Human Recombinant

Recombinant human RAET1G, produced in E. coli, is a single, non-glycosylated polypeptide chain. This chain corresponds to Isoform 2 (Uniprot accession #Q6H3X3-2) and consists of 198 amino acids, including a 10 amino acid N-terminal His tag. The calculated total molecular mass is 22.43kDa.
Shipped with Ice Packs
Cat. No.
BT14752
Source
Escherichia Coli.
Appearance
Lyophilized powder with a white color after filtration.

RAET1L Human

Retinoic Acid Early Transcript 1L Human Recombinant

This product is a recombinant human RAET1L protein produced in Sf9 insect cells using a baculovirus expression system. It consists of a single polypeptide chain with 202 amino acids (specifically, amino acids 26 to 218) and has a molecular weight of 22.9 kDa. The protein is glycosylated, meaning it has sugar molecules attached. For purification and detection purposes, a 6-amino acid histidine tag (His-tag) has been added to the C-terminus of the protein. The protein is purified using proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT14817
Source

Sf9, Baculovirus cells.

Appearance

The product appears as a clear and colorless solution that has been sterilized by filtration.

RAET1E Human, IgG-His

Retinoic Acid Early Transcript 1E Human Recombinant, IgG-His Tag

Recombinant Human RAET1E, produced in Sf9 insect cells, is a single glycosylated polypeptide chain encompassing 437 amino acids (31-225 a.a.) with a molecular weight of 49.6 kDa (observed molecular weight on SDS-PAGE may vary between 57-70 kDa). This protein is expressed with a 239 amino acid IgG-His tag at the C-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT14374
Source
Sf9, Baculovirus cells.
Appearance
A colorless, sterile-filtered solution.

RAET1E Human, Sf9

Retinoic Acid Early Transcript 1E Human Recombinant, Sf9

Recombinant human RAET1E, produced in Sf9 insect cells, is a single, glycosylated polypeptide chain consisting of 204 amino acids (residues 31-225). With a molecular weight of 23.4 kDa, it appears as a band at approximately 28-40 kDa on SDS-PAGE. The protein is expressed with a C-terminal 6-His tag and purified using proprietary chromatographic methods.

Shipped with Ice Packs
Cat. No.
BT14461
Source
Sf9, Baculovirus cells.
Appearance
A clear, colorless solution that has undergone sterile filtration.

RAET1E Human

Retinoic Acid Early Transcript 1E Human Recombinant

Recombinant human RAET1E, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 219 amino acids (residues 31-225). With a molecular weight of 24.9 kDa, it includes a 24 amino acid His-tag fused at the N-terminus. The protein is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT14596
Source
Escherichia Coli.
Appearance
Clear, sterile-filtered solution.

RAET1E Mouse

Retinoic Acid Early Transcript 1E Mouse Recombinant

Produced in Sf9 insect cells, RAET1E is a single, glycosylated polypeptide chain comprising 207 amino acids (29-227a.a.). It has a molecular weight of 23.5 kDa, but on SDS-PAGE, it appears at a size of approximately 28-40 kDa. The protein is expressed with an 8 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT14683
Source
Sf9, Insect cells.
Appearance
A clear, sterile-filtered solution.
Definition and Classification

Retinoic Acid Early Transcript 1E (RAET1E) is a cell surface glycoprotein encoded by the RAET1E gene located on chromosome 6 . RAET1E is related to MHC class I molecules but maps outside the MHC locus . It is composed of an external α1α2 domain, a transmembrane segment, and a C-terminal cytoplasmic tail .

Biological Properties

Key Biological Properties: RAET1E functions as a stress-induced ligand for the NKG2D receptor . It is involved in immune responses and pathogen recognition.

Expression Patterns and Tissue Distribution: RAET1E is expressed in various tissues, including the mucosa of the esophagus, skin, testicles, olfactory zone of the nasal mucosa, Achilles tendon, tonsils, and gonads .

Biological Functions

Primary Biological Functions: RAET1E plays a crucial role in immune responses by acting as a ligand for the NKG2D receptor, which is involved in natural killer (NK) cell-mediated cytotoxicity . It helps in the recognition and elimination of stressed or infected cells.

Role in Immune Responses and Pathogen Recognition: RAET1E is upregulated in response to cellular stress and infection, facilitating the activation of NK cells and T cells to target and destroy abnormal cells .

Modes of Action

Mechanisms with Other Molecules and Cells: RAET1E interacts with the NKG2D receptor on NK cells and some T cells . This interaction triggers the activation of these immune cells, leading to the destruction of target cells.

Binding Partners and Downstream Signaling Cascades: The binding of RAET1E to the NKG2D receptor activates downstream signaling pathways that result in the release of cytotoxic granules and cytokines, promoting the immune response .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression of RAET1E is regulated by various factors, including stress signals and cytokines . Transcriptional regulation involves the activation of specific transcription factors in response to cellular stress.

Transcriptional Regulation and Post-Translational Modifications: RAET1E expression is modulated at the transcriptional level by stress-induced transcription factors. Post-translational modifications, such as glycosylation, are essential for its proper function and stability .

Applications

Biomedical Research: RAET1E is studied for its role in immune responses and its potential as a target for immunotherapy .

Diagnostic Tools: RAET1E expression levels can be used as biomarkers for cellular stress and immune activation .

Therapeutic Strategies: Targeting RAET1E-NKG2D interactions is being explored as a therapeutic strategy for enhancing immune responses against cancer and infections .

Role in the Life Cycle

Role Throughout the Life Cycle: RAET1E plays a vital role in immune surveillance throughout life. It is involved in the recognition and elimination of stressed or infected cells from development to aging .

From Development to Aging and Disease: RAET1E expression is crucial during development for immune system maturation and continues to be important in maintaining immune homeostasis and responding to infections and cellular stress throughout life .

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