Retinoic Acid Early Transcript 1G Human Recombinant
Retinoic Acid Early Transcript 1L Human Recombinant
This product is a recombinant human RAET1L protein produced in Sf9 insect cells using a baculovirus expression system. It consists of a single polypeptide chain with 202 amino acids (specifically, amino acids 26 to 218) and has a molecular weight of 22.9 kDa. The protein is glycosylated, meaning it has sugar molecules attached. For purification and detection purposes, a 6-amino acid histidine tag (His-tag) has been added to the C-terminus of the protein. The protein is purified using proprietary chromatographic techniques.
Sf9, Baculovirus cells.
The product appears as a clear and colorless solution that has been sterilized by filtration.
Retinoic Acid Early Transcript 1E Human Recombinant, IgG-His Tag
Retinoic Acid Early Transcript 1E Human Recombinant, Sf9
Recombinant human RAET1E, produced in Sf9 insect cells, is a single, glycosylated polypeptide chain consisting of 204 amino acids (residues 31-225). With a molecular weight of 23.4 kDa, it appears as a band at approximately 28-40 kDa on SDS-PAGE. The protein is expressed with a C-terminal 6-His tag and purified using proprietary chromatographic methods.
Retinoic Acid Early Transcript 1E Human Recombinant
Retinoic Acid Early Transcript 1E Mouse Recombinant
Retinoic Acid Early Transcript 1E (RAET1E) is a cell surface glycoprotein encoded by the RAET1E gene located on chromosome 6 . RAET1E is related to MHC class I molecules but maps outside the MHC locus . It is composed of an external α1α2 domain, a transmembrane segment, and a C-terminal cytoplasmic tail .
Key Biological Properties: RAET1E functions as a stress-induced ligand for the NKG2D receptor . It is involved in immune responses and pathogen recognition.
Expression Patterns and Tissue Distribution: RAET1E is expressed in various tissues, including the mucosa of the esophagus, skin, testicles, olfactory zone of the nasal mucosa, Achilles tendon, tonsils, and gonads .
Primary Biological Functions: RAET1E plays a crucial role in immune responses by acting as a ligand for the NKG2D receptor, which is involved in natural killer (NK) cell-mediated cytotoxicity . It helps in the recognition and elimination of stressed or infected cells.
Role in Immune Responses and Pathogen Recognition: RAET1E is upregulated in response to cellular stress and infection, facilitating the activation of NK cells and T cells to target and destroy abnormal cells .
Mechanisms with Other Molecules and Cells: RAET1E interacts with the NKG2D receptor on NK cells and some T cells . This interaction triggers the activation of these immune cells, leading to the destruction of target cells.
Binding Partners and Downstream Signaling Cascades: The binding of RAET1E to the NKG2D receptor activates downstream signaling pathways that result in the release of cytotoxic granules and cytokines, promoting the immune response .
Regulatory Mechanisms Controlling Expression and Activity: The expression of RAET1E is regulated by various factors, including stress signals and cytokines . Transcriptional regulation involves the activation of specific transcription factors in response to cellular stress.
Transcriptional Regulation and Post-Translational Modifications: RAET1E expression is modulated at the transcriptional level by stress-induced transcription factors. Post-translational modifications, such as glycosylation, are essential for its proper function and stability .
Biomedical Research: RAET1E is studied for its role in immune responses and its potential as a target for immunotherapy .
Diagnostic Tools: RAET1E expression levels can be used as biomarkers for cellular stress and immune activation .
Therapeutic Strategies: Targeting RAET1E-NKG2D interactions is being explored as a therapeutic strategy for enhancing immune responses against cancer and infections .
Role Throughout the Life Cycle: RAET1E plays a vital role in immune surveillance throughout life. It is involved in the recognition and elimination of stressed or infected cells from development to aging .
From Development to Aging and Disease: RAET1E expression is crucial during development for immune system maturation and continues to be important in maintaining immune homeostasis and responding to infections and cellular stress throughout life .