Recombinant Proteins

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Collagen-VI Bovine

Bovine Collagen-VI

Bovine Collagen-VI is a naturally occurring protein extracted from bovine placenta and purified using specialized chromatographic methods.

Shipped with Ice Packs
Cat. No.
BT12997
Source

Bovine placenta.

Appearance

It appears as a white, freeze-dried powder that has been filtered.

CTHRC1 Human

Collagen Triple Helix Repeat Containing 1 Human Recombinant

Recombinant human CTHRC1, expressed in E. coli, is a single, non-glycosylated polypeptide chain composed of 234 amino acids (spanning from amino acid positions 31 to 243). It possesses a molecular weight of 25.3 kDa. The protein includes a 21 amino acid His-tag fused at its N-terminus. Purification is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT13068
Source
Escherichia Coli.
Appearance
The product appears as a clear and colorless solution that has been sterilized by filtration.

CTHRC1 Human, HEK

Collagen Triple Helix Repeat Containing 1 Human Recombinant, HEK

This product consists of the recombinant human CTHRC1 protein, produced in HEK cells. It is a single chain polypeptide with glycosylation, encompassing amino acids 31 to 243, and a 6-amino acid His tag at the C-terminus. The calculated molecular mass is 23.9 kDa.
Shipped with Ice Packs
Cat. No.
BT13138
Source
HEK 293.
Appearance
The product appears as a white powder, obtained through lyophilization after filtration.

COL4A3BP Human

Collagen Type IV Alpha 3 Binding Protein Human Recombinant

This product consists of the recombinant human COL4A3BP protein, produced in E. coli. It is a single, non-glycosylated polypeptide chain encompassing amino acids 347 to 598 of the full protein sequence, resulting in a molecular weight of 33.1 kDa. The protein includes a 38 amino acid His-Tag fused to its N-terminus to facilitate purification via proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT12117
Source
Escherichia Coli.
Appearance
The product appears as a clear, colorless solution that has been sterilized through filtration.

Collagen-I Bovine

Bovine Collagen-I

Bovine Collagen-I is a naturally occurring protein extracted and purified from bovine skin using specialized chromatographic methods.

Shipped with Ice Packs
Cat. No.
BT12200
Source

Bovine skin.

Appearance

White, lyophilized powder that has been filtered.

Collagen-I Goat

Goat Collagen-I

This product consists of Collagen-I, a natural protein, extracted from Goat tissues and purified through proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT12284
Source

Goat tissues.

Appearance

It appears as a white, lyophilized powder after filtration.

Collagen-I Mouse

Mouse Collagen-I

Mouse Collagen-I is a naturally occurring protein extracted from the tail tendon of mice. The purification process involves specialized chromatographic methods.

Shipped with Ice Packs
Cat. No.
BT12357
Source

Mouse tail tendon.

Appearance

This product appears as a white, lyophilized (freeze-dried) powder that has been filtered.

Collagen-II Bovine

Bovine Collagen-II

This product is a purified natural protein extracted from bovine knee joints using specialized chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT12427
Source

Bovine knee joint.

Appearance

It appears as a white, freeze-dried powder that has been filtered.

Collagen-II Human

Human Collagen-II

Human Collagen-II, sourced from human cartilage, undergoes a purification process using specialized chromatographic methods.

Shipped with Ice Packs
Cat. No.
BT12517
Source

Human cartilage.

Appearance

White, freeze-dried powder that has been filtered.

Collagen-III Bovine

Bovine Collagen-III

Mouse Collagen-III is obtained through a purification process involving the washing and extraction of dissected tissue with dilute acetic acid. Subsequent purification of Collagen-I is achieved using proprietary chromatographic methods.

Shipped with Ice Packs
Cat. No.
BT12574
Source

Bovine washed dissected tissue.

Appearance

White lyophilized powder (freeze-dried).

Definition and Classification

Collagen is the most abundant protein in the human body, constituting about 25-35% of the total protein content. It is a key structural protein found in the extracellular matrix of various connective tissues, including skin, bones, tendons, ligaments, and cartilage . Collagen molecules are composed of three polypeptide chains that form a triple helix structure . There are at least 28 different types of collagen, classified based on their structure and function . The most common types include:

  • Type I: Found in skin, tendon, vasculature, organs, and bone.
  • Type II: Predominantly in cartilage.
  • Type III: Present in reticular fibers, commonly alongside Type I.
  • Type IV: Forms the basal lamina of the basement membrane.
  • Type V: Found in cell surfaces, hair, and placenta .
Biological Properties

Collagen exhibits several key biological properties:

  • Expression Patterns: Collagen is synthesized primarily by fibroblasts and other specialized cells such as osteoblasts and chondrocytes .
  • Tissue Distribution: It is widely distributed across various tissues, providing structural integrity and mechanical strength. For instance, Type I collagen is abundant in skin and bones, while Type II is mainly found in cartilage .
  • Hierarchical Structure: Collagen fibers are organized into fibrils, fibers, and bundles, each contributing to the unique mechanical properties of different tissues .
Biological Functions

Collagen plays several crucial roles in the body:

  • Structural Support: It provides tensile strength and structural integrity to tissues .
  • Immune Responses: Collagen can influence immune responses by interacting with immune cells and modulating inflammation .
  • Pathogen Recognition: Certain collagen types can bind to pathogens, aiding in their recognition and clearance by the immune system .
Modes of Action

Collagen interacts with various molecules and cells through several mechanisms:

  • Binding Partners: Collagen binds to cell surface receptors such as integrins and discoidin domain receptors (DDRs), initiating signaling cascades .
  • Downstream Signaling: These interactions trigger downstream signaling pathways that regulate cell proliferation, differentiation, and migration .
  • Enzymatic Breakdown: Enzymes like collagenases break down collagen, which is crucial for tissue remodeling and repair .
Regulatory Mechanisms

The expression and activity of collagen are tightly regulated:

  • Transcriptional Regulation: Genes encoding collagen are regulated by transcription factors such as TGF-β and BMP1 .
  • Post-Translational Modifications: Collagen undergoes several post-translational modifications, including hydroxylation and glycosylation, which are essential for its stability and function .
  • Epigenetic Regulation: DNA methylation and histone modifications also play a role in regulating collagen gene expression .
Applications

Collagen has numerous applications in biomedical research and clinical practice:

  • Biomedical Research: Collagen-based scaffolds are used in tissue engineering and regenerative medicine .
  • Diagnostic Tools: Collagen biomarkers are used in diagnosing and monitoring diseases such as fibrosis and osteoarthritis .
  • Therapeutic Strategies: Collagen is used in wound healing, drug delivery systems, and as a component of medical devices .
Role in the Life Cycle

Collagen plays a vital role throughout the human life cycle:

  • Development: During development, collagen provides the necessary framework for tissue formation and organ development .
  • Aging: Collagen production decreases with age, leading to wrinkles, reduced skin elasticity, and weakened connective tissues .
  • Disease: Alterations in collagen structure and function are associated with various diseases, including fibrosis, osteoarthritis, and certain cancers .
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