Recombinant Proteins

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RCN1 Human

Reticulocalbin 1 Human Recombinant

Recombinant Human RCN1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 341 amino acids (30-331 a.a.). It has a molecular mass of 40.4 kDa. However, the actual molecular weight observed on SDS-PAGE might be slightly higher. This discrepancy arises from the fusion of a 39 amino acid His-Tag to the N-terminus of RCN1. The protein undergoes purification using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT13950
Source
Escherichia Coli.
Appearance
A clear, sterile solution.

RCN2 Human

Reticulocalbin 2 Human Recombinant

Produced in E. coli, our recombinant RCN2 protein is a single, non-glycosylated polypeptide chain. It comprises 313 amino acids (specifically, residues 26-317) and exhibits a molecular mass of 36.8 kDa. Please note that the molecular weight observed on SDS-PAGE may appear higher. For purification purposes, RCN2 is tagged with a 21 amino acid His-tag at the N-terminus and subsequently purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT14010
Source
Escherichia Coli.
Appearance
The product is a sterile-filtered solution, appearing colorless.

RCN3 Human

Reticulocalbin 3 Human Recombinant

Recombinant human RCN3, expressed in E. coli, is available as a single, non-glycosylated polypeptide chain. This protein comprises 333 amino acids, specifically spanning from amino acid positions 21 to 328. With a molecular mass of 37.9 kDa, the recombinant RCN3 includes a 25 amino acid His-tag fused at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT14085
Source
Escherichia Coli.
Appearance
The product is provided as a sterile, filtered solution, appearing colorless.
Definition and Classification

Reticulocalbin (RCN) is an endoplasmic reticulum (ER)-resident calcium-binding protein characterized by the presence of six EF-hand motifs . It belongs to the CREC family of proteins, which are involved in calcium-binding and regulation within the secretory pathway . Reticulocalbin is encoded by the RCN1 gene in humans .

Biological Properties

Key Biological Properties: Reticulocalbin is known for its calcium-binding capabilities, facilitated by its six EF-hand motifs . This protein is localized in the lumen of the ER, where it plays a role in calcium-dependent activities .

Expression Patterns: Reticulocalbin exhibits a ubiquitous expression pattern in both mouse and human tissues . It is expressed in various cell types, including endothelial and prostate cancer cell lines .

Tissue Distribution: Reticulocalbin is widely distributed across different tissues, reflecting its fundamental role in cellular functions .

Biological Functions

Primary Biological Functions: Reticulocalbin is involved in the regulation of calcium-dependent activities within the ER lumen or post-ER compartments . It may also play a role in protein folding and quality control within the ER .

Role in Immune Responses and Pathogen Recognition: While specific roles in immune responses and pathogen recognition are not well-documented, reticulocalbin’s involvement in calcium signaling suggests it may influence immune cell functions indirectly .

Modes of Action

Mechanisms with Other Molecules and Cells: Reticulocalbin interacts with various molecules within the ER, including other calcium-binding proteins and chaperones . It may also interact with signaling molecules involved in calcium homeostasis .

Binding Partners: Reticulocalbin binds to calcium ions through its EF-hand motifs, which are crucial for its function . It may also interact with other ER-resident proteins to facilitate its role in calcium regulation .

Downstream Signaling Cascades: Reticulocalbin’s role in calcium regulation suggests it may influence downstream signaling pathways that depend on calcium as a second messenger .

Regulatory Mechanisms

Transcriptional Regulation: The expression of reticulocalbin is regulated at the transcriptional level, although specific transcription factors involved are not well-characterized .

Post-Translational Modifications: Reticulocalbin undergoes post-translational modifications, including phosphorylation, which may influence its activity and interactions with other proteins .

Applications

Biomedical Research: Reticulocalbin is a subject of interest in biomedical research due to its role in calcium regulation and potential involvement in various diseases .

Diagnostic Tools: Reticulocalbin has been identified as a potential biomarker for diseases such as chronic obstructive pulmonary disease (COPD) and atherosclerosis .

Therapeutic Strategies: Targeting reticulocalbin and its pathways may offer therapeutic potential for diseases characterized by dysregulated calcium homeostasis .

Role in the Life Cycle

Development: Reticulocalbin plays a role in cellular development by regulating calcium-dependent processes essential for cell differentiation and growth .

Aging and Disease: Dysregulation of reticulocalbin expression or function has been associated with various diseases, including cancer and cardiovascular diseases . Its role in maintaining calcium homeostasis is crucial for cellular health throughout the life cycle .

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