Recombinant Proteins

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NPM1 Human

Nucleophosmin Human Recombinant

Recombinant human NPM1, produced in E. coli bacteria, is a single polypeptide chain that lacks glycosylation. It consists of 314 amino acids (specifically, amino acids 1 to 294), resulting in a molecular weight of 34.7 kDa. The NPM1 protein has a 20 amino acid His-tag attached to its N-terminus and is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT20466
Source
Escherichia Coli.
Appearance
A clear and sterile solution.

NPM2 Human

Nucleophosmin 2 Human Recombinant

Recombinant NPM2 Human, produced in E. coli, is a single polypeptide chain consisting of 237 amino acids (1-214) with a molecular mass of 26.6 kDa. It comprises NPM2 fused to a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20572
Source
E.coli.
Appearance
A sterile, filtered, and colorless solution.
Definition and Classification

Nucleophosmin (NPM), also known as nucleolar phosphoprotein B23, numatrin, or NPM1, is an abundant nucleolar protein found in the nuclei of proliferating cells . It is involved in various cellular processes, including ribosome biogenesis, mRNA processing, chromatin remodeling, and embryogenesis . NPM1 is classified as a multifunctional protein due to its diverse roles in cellular biology .

Biological Properties

Key Biological Properties: NPM1 is a critical cellular protein that participates in mRNA transport, chromatin remodeling, apoptosis, and genome stability . It functions as both a proto-oncogene and a tumor suppressor .

Expression Patterns: NPM1 is abundantly expressed in all tissues, with higher expression levels in proliferating cells .

Tissue Distribution: NPM1 is predominantly localized in the nucleolus but can also be found in the nucleoplasm and cytoplasm under certain conditions .

Biological Functions

Primary Biological Functions: NPM1 is involved in ribosome biogenesis, mRNA processing, chromatin remodeling, and embryogenesis . It also plays a crucial role in maintaining genomic stability by participating in various DNA repair pathways and regulating apoptosis .

Role in Immune Responses and Pathogen Recognition: NPM1 is implicated in immune responses and pathogen recognition, although its exact role in these processes is still being studied .

Modes of Action

Mechanisms with Other Molecules and Cells: NPM1 interacts with various molecules and cells, including histones and other chromatin remodeling proteins . It binds to single-stranded and double-stranded nucleic acids, with a preference for G-quadruplex forming nucleic acids .

Binding Partners: NPM1 has multiple binding partners, including ribonucleoprotein structures and viral basic proteins .

Downstream Signaling Cascades: NPM1 is involved in several downstream signaling cascades that regulate apoptosis, DNA repair, and chromatin remodeling .

Regulatory Mechanisms

Expression and Activity Control: The expression and activity of NPM1 are regulated through various mechanisms, including transcriptional regulation and post-translational modifications .

Transcriptional Regulation: NPM1 expression is controlled at the transcriptional level by various transcription factors .

Post-Translational Modifications: NPM1 undergoes several post-translational modifications, including phosphorylation, acetylation, and SUMOylation, which regulate its activity and localization .

Applications

Biomedical Research: NPM1 is widely studied in biomedical research due to its multifunctional roles and involvement in various cellular processes .

Diagnostic Tools: NPM1 is used as a diagnostic marker for certain cancers, particularly acute myeloid leukemia (AML), where NPM1 mutations are common .

Therapeutic Strategies: Targeting NPM1 and its pathways is being explored as a therapeutic strategy for treating cancers and other diseases .

Role in the Life Cycle

Development to Aging and Disease: NPM1 plays a crucial role throughout the life cycle, from development to aging and disease . It is essential for normal cellular function and is involved in various processes that maintain cellular homeostasis .

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