Recombinant Proteins

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DYNLRB2 Human

Dynein Light Chain Roadblock-Type 2 Human Recombinant

Recombinant human DYNLRB2, expressed in E. coli with an N-terminal 20 amino acid His tag, is a non-glycosylated polypeptide chain consisting of 116 amino acids (residues 1-96 of the DYNLRB2 sequence) with a molecular weight of 13 kDa. Purification is achieved using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT540
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterile filtered.

DYNLT1 Human

Dynein, Light Chain, Tctex-Type 1 Human Recombinant

Recombinant Human DYNLT1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 133 amino acids (1-113 a.a.). With a molecular weight of 14.6 kDa, DYNLT1 is fused to a 20 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT632
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

DYNLT3 Human

Dynein, Light Chain, Tctex-Type 3 Human Recombinant

Recombinant human DYNLT3, expressed in E. coli, is a single polypeptide chain with a molecular weight of 15.5 kDa. It consists of 139 amino acids, including a 23 amino acid His-tag fused at the N-terminus (amino acids 1-116). Purification is achieved using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT700
Source
E.coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

DNAL1 Human

Dynein Axonemal Light Chain 1 Human Recombinant

This product consists of the human DNAL1 protein, which has been recombinantly produced in E. coli bacteria. The protein is a single chain of 213 amino acids (specifically amino acids 1-190 of the full-length protein), resulting in a molecular weight of 23.9 kDa. To facilitate purification and detection, the DNAL1 protein has been engineered with a 23 amino acid His-tag at its N-terminus. The protein has been purified using proprietary chromatographic techniques to ensure its high quality and purity.
Shipped with Ice Packs
Cat. No.
BT215
Source
E.coli.
Appearance
The product is a sterile and colorless solution that has been filtered for clarity.

DNAL4 Human

Dynein Axonemal Light Chain 4 Human Recombinant

This product consists of recombinant human DNAL4 protein produced in E. coli. It is a single, non-glycosylated polypeptide chain composed of 125 amino acids (specifically, amino acids 1 to 105). With a molecular weight of 14.1 kDa, the DNAL4 protein is fused to a 20 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT300
Source
Escherichia Coli.
Appearance
The product appears as a clear, sterile-filtered solution.

DNALI1 Human

Dynein Axonemal Light Intermediate Chain 1 Human Recombinant

Recombinant human DNALI1, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 303 amino acids, with a sequence spanning from amino acid 1 to 280, and has a molecular weight of 34.2 kDa. The protein is purified using proprietary chromatographic techniques and is fused to a 23 amino acid His-tag at its N-terminus.
Shipped with Ice Packs
Cat. No.
BT333
Source
Escherichia Coli.
Appearance
A clear, sterile-filtered solution.

DYNLL1 Human

Dynein Light Chain LC8 Type-1 Human Recombinant

Recombinant Human DYNLL1, expressed in E.Coli, is a single, non-glycosylated polypeptide chain. It consists of 109 amino acids (1-89a.a.) and has a molecular weight of 12.5kDa. A 20 amino acid His-Tag is fused to the N-terminus of the DYNLL1 protein. Purification is achieved using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT358
Source
Escherichia Coli.
Appearance
A clear solution that has been sterilized by filtration.

DYNLL2 Human

Dynein Light Chain LC8 Type-2 Human Recombinant

Recombinant human DYNLL2, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 109 amino acids (1-89a.a) with a molecular weight of 12.5kDa. The protein is expressed with a 20 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT414
Source
Escherichia Coli.
Appearance
A clear solution that has been sterilized by filtration.

DYNLRB1 Human

Dynein Light Chain Roadblock-Type 1 Human Recombinant

Recombinant Human DYNLRB1, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 104 amino acids (residues 1-96). It has a molecular weight of 11.9 kDa. The DYNLRB1 protein is fused to an 8 amino acid His-Tag at its C-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT483
Source
Escherichia Coli.
Appearance
Clear, colorless, and sterile-filtered solution.
Definition and Classification

Dynein light chains (DLCs) are small, non-catalytic subunits of the dynein motor protein complex. Dyneins are a family of cytoskeletal motor proteins that move along microtubules in cells, converting chemical energy stored in ATP to mechanical work . Dyneins are classified into two main groups: cytoplasmic dyneins and axonemal dyneins . DLCs are further classified into several types, including DYNLL1, DYNLL2, DYNLRB1, DYNLRB2, DYNLT1, and DYNLT3 .

Biological Properties

Key Biological Properties: Dynein light chains are essential for the structural integrity and function of the dynein motor complex. They play a role in stabilizing the dynein complex and facilitating its interaction with various cargoes .

Expression Patterns and Tissue Distribution: DLCs are ubiquitously expressed in various tissues, with specific expression patterns depending on the type of DLC. For example, DYNLL1 and DYNLL2 are expressed in a wide range of tissues, including the brain, heart, and skeletal muscles .

Biological Functions

Primary Biological Functions: Dynein light chains are involved in intracellular transport, mitosis, and the movement of cilia and flagella . They facilitate the transport of various cellular cargoes, including organelles, vesicles, and proteins, towards the minus-end of microtubules .

Role in Immune Responses and Pathogen Recognition: DLCs play a role in immune responses by regulating the transport of immune-related vesicles and proteins. They are also involved in the recognition and transport of pathogens within cells .

Modes of Action

Mechanisms with Other Molecules and Cells: Dynein light chains interact with other subunits of the dynein complex, including heavy chains, intermediate chains, and light intermediate chains . They also interact with various adaptor proteins that link dynein to its cargoes .

Binding Partners and Downstream Signaling Cascades: DLCs bind to a variety of proteins, including neuronal nitric oxide synthase and Bcl-2-associated athanogene 1 (BAG1) . These interactions are crucial for the regulation of downstream signaling pathways involved in cell survival, apoptosis, and intracellular transport .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of dynein light chains are regulated at multiple levels, including transcriptional regulation and post-translational modifications . Transcription factors such as ASCIZ (ATMIN/ZNF822) directly regulate the expression of DLCs .

Post-Translational Modifications: DLCs undergo various post-translational modifications, including phosphorylation, which modulate their activity and interactions with other proteins .

Applications

Biomedical Research: Dynein light chains are used as molecular tools to study intracellular transport and motor protein function . They are also used in research to understand the mechanisms of diseases related to dynein dysfunction, such as neurodegenerative diseases .

Diagnostic Tools and Therapeutic Strategies: DLCs have potential applications in diagnostic tools for detecting dynein-related dysfunctions. They are also being explored as therapeutic targets for diseases involving defects in intracellular transport and motor protein function .

Role in the Life Cycle

Role Throughout the Life Cycle: Dynein light chains play a crucial role throughout the life cycle, from development to aging and disease . During development, they are involved in processes such as cell division, differentiation, and migration . In aging and disease, dynein dysfunction can lead to various pathological conditions, including neurodegenerative diseases and impaired immune responses .

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