Recombinant Proteins

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Product List

DNAL1 Human

Dynein Axonemal Light Chain 1 Human Recombinant

DNAL1 Human Recombinant produced in E. coli is a single polypeptide chain containing 213 amino acids (1-190) and having a molecular mass of 23.9 kDa. DNAL1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT215
Source
E.coli.
Appearance
Sterile Filtered colorless solution.

DNAL4 Human

Dynein Axonemal Light Chain 4 Human Recombinant

DNAL4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 125 amino acids (1-105 a.a) and having a molecular mass of 14.1kDa.
DNAL4 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT300
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

DNALI1 Human

Dynein Axonemal Light Intermediate Chain 1 Human Recombinant

DNALI1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 303 amino acids (1-280 a.a) and having a molecular mass of 34.2kDa. DNALI1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT333
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

DYNLL1 Human

Dynein Light Chain LC8 Type-1 Human Recombinant

DYNLL1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 109 amino acids (1-89a.a.) and having a molecular mass of 12.5kDa. The DYNLL1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT358
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

DYNLL2 Human

Dynein Light Chain LC8 Type-2 Human Recombinant

DYNLL2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 109 amino acids (1-89a.a) and having a molecular mass of 12.5kDa.
DYNLL2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT414
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

DYNLRB1 Human

Dynein Light Chain Roadblock-Type 1 Human Recombinant

DYNLRB1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 104 amino acids (1-96 a.a.) and having a molecular mass of 11.9 kDa. The DYNLRB1 is fused to an 8 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT483
Source
Escherichia Coli.
Appearance
Sterile filtered colorless solution.

DYNLRB2 Human

Dynein Light Chain Roadblock-Type 2 Human Recombinant

DYNLRB2 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 116 amino acids (1-96 a.a.) and having a molecular mass of 13kDa. The DYNLRB2 is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT540
Source
Escherichia Coli.
Appearance
Sterile Filtered colorless solution.

DYNLT1 Human

Dynein, Light Chain, Tctex-Type 1 Human Recombinant

DYNLT1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 133 amino acids (1-113 a.a.) and having a molecular mass of 14.6kDa.
DYNLT1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT632
Source
Escherichia Coli.
Appearance
Sterile Filtered colorless solution.

DYNLT3 Human

Dynein, Light Chain, Tctex-Type 3 Human Recombinant

DYNLT3 Human Recombinant produced in E. coli is a single polypeptide chain containing 139 amino acids (1-116) and having a molecular mass of 15.5 kDa.
DYNLT3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT700
Source
E.coli.
Appearance
Sterile Filtered colorless solution.

Introduction

Definition and Classification

Dynein light chains (DLCs) are small, non-catalytic subunits of the dynein motor protein complex. Dyneins are a family of cytoskeletal motor proteins that move along microtubules in cells, converting chemical energy stored in ATP to mechanical work . Dyneins are classified into two main groups: cytoplasmic dyneins and axonemal dyneins . DLCs are further classified into several types, including DYNLL1, DYNLL2, DYNLRB1, DYNLRB2, DYNLT1, and DYNLT3 .

Biological Properties

Key Biological Properties: Dynein light chains are essential for the structural integrity and function of the dynein motor complex. They play a role in stabilizing the dynein complex and facilitating its interaction with various cargoes .

Expression Patterns and Tissue Distribution: DLCs are ubiquitously expressed in various tissues, with specific expression patterns depending on the type of DLC. For example, DYNLL1 and DYNLL2 are expressed in a wide range of tissues, including the brain, heart, and skeletal muscles .

Biological Functions

Primary Biological Functions: Dynein light chains are involved in intracellular transport, mitosis, and the movement of cilia and flagella . They facilitate the transport of various cellular cargoes, including organelles, vesicles, and proteins, towards the minus-end of microtubules .

Role in Immune Responses and Pathogen Recognition: DLCs play a role in immune responses by regulating the transport of immune-related vesicles and proteins. They are also involved in the recognition and transport of pathogens within cells .

Modes of Action

Mechanisms with Other Molecules and Cells: Dynein light chains interact with other subunits of the dynein complex, including heavy chains, intermediate chains, and light intermediate chains . They also interact with various adaptor proteins that link dynein to its cargoes .

Binding Partners and Downstream Signaling Cascades: DLCs bind to a variety of proteins, including neuronal nitric oxide synthase and Bcl-2-associated athanogene 1 (BAG1) . These interactions are crucial for the regulation of downstream signaling pathways involved in cell survival, apoptosis, and intracellular transport .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression and activity of dynein light chains are regulated at multiple levels, including transcriptional regulation and post-translational modifications . Transcription factors such as ASCIZ (ATMIN/ZNF822) directly regulate the expression of DLCs .

Post-Translational Modifications: DLCs undergo various post-translational modifications, including phosphorylation, which modulate their activity and interactions with other proteins .

Applications

Biomedical Research: Dynein light chains are used as molecular tools to study intracellular transport and motor protein function . They are also used in research to understand the mechanisms of diseases related to dynein dysfunction, such as neurodegenerative diseases .

Diagnostic Tools and Therapeutic Strategies: DLCs have potential applications in diagnostic tools for detecting dynein-related dysfunctions. They are also being explored as therapeutic targets for diseases involving defects in intracellular transport and motor protein function .

Role in the Life Cycle

Role Throughout the Life Cycle: Dynein light chains play a crucial role throughout the life cycle, from development to aging and disease . During development, they are involved in processes such as cell division, differentiation, and migration . In aging and disease, dynein dysfunction can lead to various pathological conditions, including neurodegenerative diseases and impaired immune responses .

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