Recombinant Proteins

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Vimentin Human

Vimentin Human Recombinant

Recombinant Human Vimentin, expressed in E. coli, is a non-glycosylated single polypeptide chain protein. It has a molecular weight of 53.5 kDa and consists of 465 amino acids. The protein undergoes purification using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT25715
Source
Escherichia Coli.
Appearance
White, sterile-filtered, lyophilized powder.

Vimentin Bovine

Bovine Vimentin

Bovine Vimentin protein, with a molecular weight of 57 kDa, produced in Bovine Lens.
Shipped with Ice Packs
Cat. No.
BT25628
Source
Bovine Lens.
Appearance
Sterile Filtered White lyophilized powder.
Definition and Classification

Vimentin is a type III intermediate filament (IF) protein predominantly expressed in mesenchymal cells . It is a crucial component of the cytoskeleton, providing structural support and maintaining cellular integrity . Vimentin is encoded by the VIM gene located on chromosome 10 in humans .

Biological Properties

Key Biological Properties: Vimentin plays a significant role in maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions . It is involved in various cellular processes, including cell migration, cell shape, and organelle anchorage .

Expression Patterns: Vimentin is widely expressed in mesenchymal cells and is also found in endothelial cells, fibroblasts, and certain types of epithelial cells . It is highly dynamic and can be found in different cellular locations, including the cytoplasm and cell surface .

Tissue Distribution: Vimentin is expressed in various tissues, including the endothelial cells of lymphatic vessels, dermis, and stroma of bone marrow . It is also present in the ventricular zone, descending thoracic aorta, and synovial membrane .

Biological Functions

Primary Biological Functions: Vimentin is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions . It plays a role in cell migration, cell shape, and plasticity .

Role in Immune Responses and Pathogen Recognition: Vimentin is involved in immune responses by regulating leukocyte migration and cell attachment to vascular endothelium . It also participates in pathogen recognition and immune-inflammatory responses .

Modes of Action

Mechanisms with Other Molecules and Cells: Vimentin interacts with various molecules and cells, including integrins and adhesion receptors . It regulates cell adhesion by interacting with and regulating integrin function .

Binding Partners and Downstream Signaling Cascades: Vimentin binds to cytoplasmic domains of adhesion receptors such as integrins, promoting the association with the extracellular matrix (ECM) . It also regulates downstream signaling cascades involved in cell migration and adhesion .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: Vimentin expression is regulated at both the transcriptional and post-transcriptional levels . Post-transcriptional regulation involves microRNAs (miRNAs) and other regulatory molecules .

Transcriptional Regulation and Post-Translational Modifications: Vimentin undergoes various post-translational modifications, including phosphorylation, which affects its function and interaction with other proteins . These modifications play a crucial role in regulating vimentin’s activity and stability .

Applications

Biomedical Research: Vimentin is widely used as a marker for mesenchymal cells in biomedical research . It is also used to study cell migration, cell shape, and cytoskeletal dynamics .

Diagnostic Tools: Vimentin is used as a diagnostic marker for certain types of cancers, including prostate cancer, gastrointestinal tumors, and breast cancer . It helps in distinguishing between different types of tumors and understanding their progression .

Therapeutic Strategies: Vimentin is a potential therapeutic target for cancer treatment . Various strategies, including antibodies, nanobodies, and miRNAs, are being explored to inhibit vimentin’s pro-tumorigenic effects .

Role in the Life Cycle

Role Throughout the Life Cycle: Vimentin plays a crucial role throughout the life cycle, from development to aging and disease . It is involved in various processes, including cell migration, cell shape, and organelle anchorage .

Development to Aging and Disease: Vimentin’s expression and function are regulated during development, and its dysregulation is associated with various diseases, including cancer, cataracts, Crohn’s disease, and rheumatoid arthritis . It also plays a role in viral infections by facilitating virus invasion and replication .

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