Recombinant Proteins

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LAMP1 Human

Lysosomal-Associated Membrane Protein Human 1 Recombinant

Produced in Sf9 Baculovirus cells, LAMP1 is a single, glycosylated polypeptide chain consisting of 363 amino acids (29-382a.a.). It has a molecular weight of 39.4kDa, although it appears between 57-70kDa on SDS-PAGE. This LAMP1 protein is expressed with a 9 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT4032
Source

Sf9, Baculovirus cells.

Appearance

The product is a sterile filtered solution, colorless in appearance.

LAMP2 Mouse

Lysosomal-Associated Membrane Protein 2 Mouse Recombinant

Recombinant LAMP2 protein, derived from mice, is produced in Sf9 Baculovirus cells. This glycosylated polypeptide chain consists of 362 amino acids (26-379 a.a.), with a molecular mass of 40.2kDa. Notably, on SDS-PAGE, the protein appears at a size of approximately 50-70kDa. The LAMP2 protein is expressed with an 8 amino acid His tag at the C-Terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT4175
Source
Sf9, Baculovirus cells.
Appearance
Clear solution, sterile filtered.

LAMP2 Human

Lysosomal-Associated Membrane Protein 2 Human Recombinant

LAMP2 is a full-length cDNA that encodes the human lysosomal-associated membrane protein 2. This protein has a molecular mass of 42,488 Daltons at a pH of 5.88. A deca-histidine purification tag is fused to the LAMP2 protein.
Shipped with Ice Packs
Cat. No.
BT4240
Source
Sf9 insect cells.

LAMP3 Human

Lysosomal-Associated Membrane Protein 3 Human Recombinant

LAMP3, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain composed of 362 amino acids (28-381a.a.) with a molecular mass of 38.8 kDa. (Molecular size on SDS-PAGE is estimated to be between 57-70 kDa). LAMP3 is expressed with an 8 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT4464
Source
Sf9, Baculovirus cells.
Appearance
Sterile Filtered colorless solution.
Definition and Classification

Lysosomal-Associated Membrane Proteins (LAMPs) are a family of glycoproteins primarily located on the lysosomal membrane. The most well-known members of this family include LAMP-1, LAMP-2, and LAMP-3 (also known as DC-LAMP). These proteins play crucial roles in maintaining lysosomal integrity and function .

Biological Properties

Key Biological Properties: LAMPs are highly glycosylated proteins, which protect them from degradation by lysosomal enzymes. They are integral membrane proteins with a large luminal domain and a short cytoplasmic tail .

Expression Patterns: LAMP-1 and LAMP-2 are ubiquitously expressed in various tissues, while LAMP-3 is more specific to dendritic cells .

Tissue Distribution: LAMP-1 and LAMP-2 are found in almost all cell types, with high expression in cells involved in immune responses, such as macrophages and dendritic cells .

Biological Functions

Primary Biological Functions: LAMPs are involved in maintaining lysosomal stability, pH regulation, and catabolism. They also play roles in autophagy and phagocytosis .

Role in Immune Responses: LAMPs, particularly LAMP-1 and LAMP-2, are involved in the immune response by participating in antigen presentation and pathogen recognition .

Modes of Action

Mechanisms with Other Molecules and Cells: LAMPs interact with various molecules, including selectins and other glycan-binding proteins. These interactions are crucial for lysosomal function and cellular signaling .

Binding Partners: LAMPs bind to carbohydrate ligands, which are essential for their role in immune responses and cellular communication .

Downstream Signaling Cascades: LAMPs are involved in signaling pathways that regulate lysosomal biogenesis, autophagy, and immune responses .

Regulatory Mechanisms

Regulatory Mechanisms: The expression and activity of LAMPs are regulated at multiple levels, including transcriptional regulation and post-translational modifications .

Transcriptional Regulation: LAMP genes are regulated by various transcription factors that respond to cellular stress and nutrient availability .

Post-Translational Modifications: LAMPs undergo extensive glycosylation, which is crucial for their stability and function .

Applications

Biomedical Research: LAMPs are used as markers for lysosomal function and integrity in various research studies .

Diagnostic Tools: LAMPs, particularly LAMP-1, are used as biomarkers for lysosomal storage disorders and other diseases .

Therapeutic Strategies: Targeting LAMPs has potential therapeutic applications in treating lysosomal storage disorders and modulating immune responses .

Role in the Life Cycle

Development to Aging and Disease: LAMPs play essential roles throughout the life cycle, from development to aging. They are involved in cellular homeostasis, development, and aging processes. Dysregulation of LAMPs is associated with various diseases, including neurodegenerative disorders and cancer .

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