Recombinant Proteins

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Cadherin-E Human

Cadherin-E Human Recombinant

Recombinant human Cadherin-E (amino acids 600-707) expressed in E. coli, exhibiting a molecular weight of 38 kDa on SDS-PAGE. Purification of Cadherin-E is achieved using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT27412
Source
Escherichia Coli.
Appearance
A clear, sterile-filtered solution.

CDH1 Human, HEK

E-Cadherin Human Recombinant, HEK

Recombinant Human E-Cadherin, expressed in HEK cells, is a secreted protein encompassing amino acids Asp155 to Ile707 of the human E-Cadherin sequence. It is produced with a C-terminal 6xHis tag.
Shipped with Ice Packs
Cat. No.
BT27516
Source
HEK cells.
Appearance
Sterile, filtered, white lyophilized powder.

CDH1 Human, Sf9

E-Cadherin Human Recombinant, Sf9

CDH1, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain consisting of 694 amino acids (24-709a.a.) with a molecular mass of 76.6 kDa. Note that on SDS-PAGE, the molecular size will appear around 70 kDa. This CDH1 protein is expressed with an 8 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT27611
Source
Sf9, Baculovirus cells.
Appearance
Sterile Filtered colorless solution.

CDH11 Human

Cadherin 11 Human Recombinant

Recombinant human CDH11, produced in E. coli, is a non-glycosylated polypeptide chain with 601 amino acids (residues 54-617). It has a molecular weight of 66.2 kDa. The protein consists of a 37 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT27688
Source
E.coli.
Appearance
A sterile, colorless, and clear solution.

CDH2 Human

Cadherin 2 Human Recombinant

Recombinant human CDH2, produced in Sf9 insect cells using a baculovirus expression system, is a single, glycosylated polypeptide chain. This protein comprises 574 amino acids (residues 160-724), including a C-terminal 6-His tag, and has a molecular weight of 62.9 kDa (appears as 70-100 kDa on SDS-PAGE). Purification is achieved through proprietary chromatographic methods.

Shipped with Ice Packs
Cat. No.
BT27781
Source

Sf9, Baculovirus cells.

Appearance
Sterile, colorless solution.

CDH5 Human

Cadherin 5 Human Recombinant

Recombinant Human Cadherin 5, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain with 810 amino acids (29-599 a.a.). It has a molecular mass of 91.5kDa and migrates at 70-100kDa on SDS-PAGE under reducing conditions. This CDH5 protein is expressed with a C-terminal 239 amino acid hIgG-His-tag and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT27844
Source
Sf9, Insect cells.
Appearance
A sterile, colorless solution that has been filtered.

CDH5 Mouse

Cadherin 5 Mouse Recombinant

Produced in Sf9 Insect cells, CDH5 is a single, glycosylated polypeptide chain with a molecular weight of 66.2 kDa (25-599 amino acids). It appears as a band at approximately 70-100 kDa on SDS-PAGE. The protein is expressed with an 8 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT27923
Source

Sf9, Insect cells.

Appearance
A clear, colorless solution that has been sterilized by filtration.

CDHR5 Mouse

Cadherin Related Family Member 5 Mouse Recombinant

Recombinant CDHR5 Mouse protein was expressed in Sf9 Baculovirus cells. It is a single, glycosylated polypeptide chain consisting of 621 amino acids (29-641 a.a), with a molecular weight of 66.3 kDa. The protein includes an 8 amino acid His-tag at the C-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT28012
Source

Sf9, Baculovirus cells.

Appearance
Clear, colorless solution, sterile filtered.
Definition and Classification

Cadherins are a class of type-1 transmembrane proteins that play a crucial role in cell-cell adhesion. They are calcium-dependent adhesion molecules, meaning their adhesive function is regulated by the presence of calcium ions . Cadherins are classified into several types based on their structure and function:

  • Classical Cadherins: These include E-cadherin, N-cadherin, and P-cadherin, which are primarily involved in maintaining cell-cell adhesion in epithelial, neural, and placental tissues, respectively .
  • Desmosomal Cadherins: These include desmogleins and desmocollins, which are involved in forming desmosomes that provide mechanical strength to tissues .
  • Protocadherins: These are primarily involved in neural development and function .
  • Atypical Cadherins: These include molecules like CELSR1, which are involved in transmitting developmental signals rather than adhesion .
Biological Properties

Cadherins exhibit several key biological properties:

  • Expression Patterns: Cadherins are expressed in a tissue-specific manner. For example, E-cadherin is predominantly found in epithelial tissues, while N-cadherin is expressed in neural and mesenchymal tissues .
  • Tissue Distribution: Cadherins are widely distributed across various tissues, including epithelial, neural, and endothelial tissues .
  • Key Biological Properties: Cadherins mediate cell-cell adhesion, maintain tissue architecture, and regulate cell polarity and migration .
Biological Functions

Cadherins serve several primary biological functions:

  • Cell-Cell Adhesion: Cadherins mediate homophilic cell-cell adhesion, meaning they bind to the same type of cadherin on adjacent cells .
  • Tissue Morphogenesis: During development, cadherins play a critical role in tissue morphogenesis by regulating cell sorting and tissue organization .
  • Immune Responses and Pathogen Recognition: Cadherins are involved in immune responses by mediating interactions between immune cells and other cell types .
Modes of Action

Cadherins interact with other molecules and cells through several mechanisms:

  • Binding Partners: Cadherins bind to catenins (α-, β-, and γ-catenins) on the intracellular side, linking them to the actin cytoskeleton .
  • Downstream Signaling Cascades: Cadherin-catenin complexes can activate various signaling pathways, including the Wnt/β-catenin pathway, which regulates gene expression and cell behavior .
Regulatory Mechanisms

The expression and activity of cadherins are regulated through multiple mechanisms:

  • Transcriptional Regulation: Cadherin gene expression is controlled by transcription factors and signaling pathways that respond to developmental and environmental cues .
  • Post-Translational Modifications: Cadherins undergo post-translational modifications such as phosphorylation, which can modulate their adhesive activity and interactions with other proteins .
Applications

Cadherins have several applications in biomedical research and clinical practice:

  • Biomedical Research: Cadherins are used as markers to study cell adhesion, tissue development, and cancer progression .
  • Diagnostic Tools: Altered cadherin expression is used as a diagnostic marker for various cancers and other diseases .
  • Therapeutic Strategies: Targeting cadherin interactions and signaling pathways is being explored as a therapeutic strategy for cancer and other diseases .
Role in the Life Cycle

Cadherins play a vital role throughout the life cycle:

  • Development: During embryonic development, cadherins are essential for tissue formation and organogenesis .
  • Aging: Cadherin function can be altered with aging, affecting tissue integrity and function .
  • Disease: Dysregulation of cadherin expression and function is associated with various diseases, including cancer and neurodegenerative disorders .
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