Recombinant Proteins

p53
LBP
CEA
HLA
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NPM
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Bax
BID

IgG Mouse

Immunoglobulin Heavy Chain Constant Region Gamma 2a Mouse Recombinant

Recombinant Mouse IgG, produced in Hi-5 cells, is a single polypeptide chain comprising 236 amino acids (98-330 a.a) with a molecular weight of 26.6 kDa. The purification of IgG is achieved using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT22878
Source
Hi-5 cells.
Appearance
Sterile, colorless solution after filtration.

IgG Mouse, His

Immunoglobulin Heavy Chain Constant Region Gamma 2a Mouse Recombinant, His Tag

Recombinant Mouse IgG, produced in Hi-5 cells, is a single polypeptide chain comprising 242 amino acids (98-330 a.a) with a molecular weight of 27.5 kDa. The IgG protein is tagged with a 6-amino acid His-tag at the C-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT22966
Source
Hi-5 cells.
Appearance
Clear, colorless, and sterile-filtered solution.

IgG1 Fc Human

Immunoglobulin Heavy Constant Gamma 1 Human Recombinant

Recombinant Human IgG1 Fc, produced in HEK cells, is a single polypeptide chain consisting of 231 amino acids (residues 100-330) with a molecular weight of 25.9 kDa. Purification of IgG1 Fc is achieved using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT23048
Source

HEK293 Cells

Appearance
Sterile Filtered colorless solution.

IGJ Human

Immunoglobulin J Human Recombinant

Recombinant human IGJ, produced in E. coli, is a single polypeptide chain consisting of 160 amino acids (residues 23-159) with a molecular weight of 18 kDa. The protein includes a 23-amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT23141
Source
E.coli.
Appearance
Clear, colorless solution, sterile-filtered.

LILRB2 Human

Leukocyte Immunoglobulin Like Receptor B2 Human Recombinant

Recombinant human LILRB2, produced in HEK293 cells, is a single, glycosylated polypeptide chain. It comprises 444 amino acids (24-461 a.a), has a molecular weight of 48.3kDa, and includes a 6 amino acid His tag at the C-terminus. Purification is achieved through proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT23536
Source

HEK293 Cells.

Appearance

A clear, colorless solution that has been sterilized by filtration.

IGLL1 Human

Immunoglobulin Lambda-Like Polypeptide 1 Human Recombinant

Recombinant Human IGLL1, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 199 amino acids (38-213a.a.). This protein, with a molecular weight of 21.5 kDa, features a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT23221
Source
E.coli.
Appearance
Clear, colorless, and sterile-filtered solution.

IgM Human

Immunoglobulin-M Human

Human Immunoglobulin-M is a protein found in human plasma. It has a molecular mass of 950kDa.

Shipped with Ice Packs
Cat. No.
BT23312
Source

Human plasma.

Appearance

A solution that has been sterilized by filtration.

IGSF8 Human

Immunoglobulin Superfamily Member 8 Human Recombinant

Recombinant human IGSF8, produced in Sf9 insect cells using a baculovirus expression system, is a single, glycosylated polypeptide chain. This protein consists of 561 amino acids (28-579a.a.) with a molecular weight of 59.6kDa. However, it appears as a band at approximately 50-70kDa on SDS-PAGE due to glycosylation. The recombinant IGSF8 contains a 6-amino acid His tag at its C-terminus and is purified through proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT23389
Source

Sf9, Baculovirus cells.

Appearance
Clear, colorless solution that has been sterilized by filtration.

LILRB1 Human

Leukocyte Immunoglobulin Like Receptor B1 Human Recombinant

Recombinant human LILRB1, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It comprises 446 amino acids (24-461 a.a), resulting in a molecular weight of 48.5 kDa. The protein includes an 8 amino acid His-tag at the C-terminus and is purified using proprietary chromatographic methods.

Shipped with Ice Packs
Cat. No.
BT23460
Source

Sf9, Baculovirus cells.

Appearance
A clear, colorless solution that has been sterilized by filtration.
Definition and Classification

The Immunoglobulin (Ig) Heavy Chain Constant Region is a part of the antibody molecule that remains constant across different antibodies of the same class. Antibodies, also known as immunoglobulins, are glycoprotein molecules produced by plasma cells. They are composed of two identical heavy chains and two identical light chains. The heavy chains determine the class or isotype of the antibody, which includes IgG, IgA, IgM, IgD, and IgE . Each class has a distinct heavy chain: γ (gamma) for IgG, α (alpha) for IgA, μ (mu) for IgM, δ (delta) for IgD, and ε (epsilon) for IgE .

Biological Properties

The constant region of the heavy chain is crucial for the antibody’s effector functions. It is composed of multiple immunoglobulin domains, typically three or four, depending on the class . The expression patterns and tissue distribution of these regions vary. For instance, IgG is the most abundant in serum and extracellular fluid, while IgA is primarily found in mucosal areas such as the gut, respiratory tract, and urogenital tract . IgM is usually the first antibody produced in response to an infection and is found in the blood and lymphatic fluid .

Biological Functions

The primary biological functions of the Ig Heavy Chain Constant Region include mediating effector functions such as complement activation, binding to Fc receptors on immune cells, and facilitating phagocytosis . These functions are essential for the immune response, including pathogen recognition and clearance. The constant region also determines the half-life of the antibody and its ability to cross the placenta, providing passive immunity to the fetus .

Modes of Action

The constant region interacts with various molecules and cells to exert its functions. It binds to Fc receptors on immune cells, such as macrophages, neutrophils, and natural killer cells, triggering downstream signaling cascades that lead to phagocytosis, antibody-dependent cellular cytotoxicity, and release of inflammatory mediators . The constant region also interacts with complement proteins, leading to the formation of the membrane attack complex and lysis of pathogens .

Regulatory Mechanisms

The expression and activity of the Ig Heavy Chain Constant Region are tightly regulated at multiple levels. Transcriptional regulation involves various enhancers and promoters that control the expression of heavy chain genes . Post-translational modifications, such as glycosylation, are crucial for the stability and function of the antibody . Class switch recombination (CSR) is another regulatory mechanism that allows B cells to switch from producing one class of antibody to another, enabling a more effective immune response .

Applications

The Ig Heavy Chain Constant Region has numerous applications in biomedical research, diagnostics, and therapeutics. Monoclonal antibodies, which are engineered to have specific constant regions, are used in the treatment of various diseases, including cancers, autoimmune disorders, and infectious diseases . Diagnostic tools, such as ELISA and Western blot, rely on antibodies to detect specific antigens . Research on the constant region also aids in the development of vaccines and understanding immune mechanisms .

Role in the Life Cycle

Throughout the life cycle, the Ig Heavy Chain Constant Region plays a critical role in immune defense. During development, it is involved in the maturation of B cells and the establishment of the immune repertoire . In adulthood, it contributes to the immune response against pathogens and the maintenance of immune memory . In aging, changes in the expression and function of the constant region can affect immune competence and susceptibility to infections and autoimmune diseases .

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