LIN28-TAT Human Recombinant
Recombinant Human LIN28, expressed in E.Coli, is a single, non-glycosylated polypeptide chain composed of 222 amino acids. This includes a 13-residue C-terminal TAT peptide, contributing to a molecular weight of 24.4kDa.
Purification of LIN28 is achieved using proprietary chromatographic methods.
LIN7A Human Recombinant
LIN7B Human Recombinant
LIN7C Human Recombinant
LIN28 Human Recombinant
LIN proteins, particularly LIN28, are RNA-binding proteins that play crucial roles in various biological processes. They are evolutionarily conserved and are known for their involvement in regulating developmental timing, pluripotency, and oncogenesis . LIN28 proteins are classified based on their RNA-binding domains, which include a cold-shock domain (CSD) and a zinc-knuckle domain (ZKD) .
Key Biological Properties: LIN28 proteins are best known for their role in promoting pluripotency by regulating the microRNA let-7 . They are highly expressed in embryonic stem cells and undifferentiated tissues and are downregulated as development and cellular differentiation proceed .
Expression Patterns: LIN28 is expressed early during development in various species, including humans, mice, C. elegans, Xenopus, zebrafish, and Drosophila . It is also highly expressed in embryonic stem cells and is downregulated in response to differentiation .
Tissue Distribution: LIN28 is predominantly found in undifferentiated tissues and embryonic stem cells. It is also expressed in certain cancers, where its expression is associated with less differentiated, more aggressive tumors .
Primary Biological Functions: LIN28 proteins are crucial for maintaining pluripotency and regulating the timing of developmental events . They play a significant role in the reprogramming of mammalian somatic cells to pluripotent cells .
Role in Immune Responses and Pathogen Recognition: While LIN28’s primary functions are related to development and pluripotency, its role in immune responses and pathogen recognition is not well-documented. However, its involvement in regulating cellular differentiation and proliferation may indirectly influence immune functions .
Mechanisms with Other Molecules and Cells: LIN28 proteins interact with microRNAs, particularly the let-7 family, to regulate their biogenesis . They bind to the let-7 pre-microRNA and block the production of mature let-7 microRNA .
Binding Partners and Downstream Signaling Cascades: LIN28 binds to and enhances the translation of the IGF-2 (insulin-like growth factor 2) mRNA . It also interacts with other mRNAs to modulate their translation and splicing events .
Transcriptional Regulation: LIN28 expression is tightly regulated during development. It is highly expressed in early development and downregulated as differentiation proceeds .
Post-Translational Modifications: LIN28 activity is regulated by various post-translational modifications, including phosphorylation and ubiquitination, which influence its stability and interaction with other molecules .
Biomedical Research: LIN28 is widely studied for its role in stem cell biology and cancer research. It is a key factor in the reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) .
Diagnostic Tools: LIN28 expression levels can serve as biomarkers for certain cancers, indicating tumor aggressiveness and differentiation status .
Therapeutic Strategies: Targeting the LIN28/let-7 pathway holds potential for cancer therapy, as it regulates multiple oncogenic proteins .
Development: LIN28 is essential for early embryonic development and maintaining pluripotency in stem cells .
Aging and Disease: Aberrant expression of LIN28 is associated with various cancers and metabolic disorders. It can influence glucose homeostasis and resistance to obesity and type 2 diabetes .