LIN7B Human
Description
Overview of LIN7B
LIN7B (UniProt ID: Q9HAP6) is a 207-amino acid protein belonging to the lin-7 family . It functions as a core component of membrane-associated multiprotein complexes that regulate the asymmetric distribution of ion channels, receptors, and transporters in polarized cells, such as neurons and epithelial cells .
Predicted Functional Partners:
Data derived from STRING interaction network and experimental studies .
Neuronal Development
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Axon Guidance: LIN7B knockdown in murine models disrupts interhemispheric axon growth, particularly in cortical neurons .
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Synaptic Plasticity: Stabilizes GRIN2B (NMDA receptor subunit) at postsynaptic membranes, modulating excitatory signaling .
Epithelial Polarization
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Directs basolateral localization of Kir2 potassium channels, GABA transporters (SLC6A12), and ERBB receptors .
Autism Spectrum Disorders (ASD)
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Genetic Evidence:
Cancer Associations
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Expression Profiles: Elevated in glioblastoma and colorectal cancer, with RNA-seq data from TCGA suggesting prognostic relevance .
Research Tools and Models
Product Specs
Product Science Overview
LIN7B plays a crucial role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. It forms membrane-associated multiprotein complexes that regulate the delivery and recycling of proteins to the correct membrane domains . Specifically, the tripartite complex composed of LIN7 (LIN7A, LIN7B, or LIN7C), CASK, and APBA1 associates with the motor protein KIF17 to transport vesicles containing N-methyl-D-aspartate (NMDA) receptor subunit NR2B along microtubules .
This complex ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin . Additionally, LIN7B is required to localize Kir2 channels, GABA transporter (SLC6A12), and EGFR/ERBB1, ERBB2, ERBB3, and ERBB4 to the basolateral membrane of epithelial cells .
Recombinant human LIN7B protein is typically produced in Escherichia coli and purified using conventional chromatography techniques . The recombinant protein often includes an N-terminal His-tag and corresponds to the amino acids 1-207 of the human LIN7B . It is used in various research applications, including studies on protein-protein interactions, cell signaling, and synaptic function.
- Source: E. coli
- Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAALVEP LGLERDVSRA VELLERLQRS GELPPQKLQA LQRVLQSRFC SAIREVYEQL YDTLDITGSA EIRAHATAKA TVAAFTASEG HAHPRVVELP KTDEGLGFNI MGGKEQNSPI YISRVIPGGV ADRHGGLKRG DQLLSVNGVS VEGEQHEKAV ELLKAAQGSV KLVVRYTPRV LEEMEARFEK MRSARRRQQH QSYSSLESRG
- Purity: >90%, by SDS-PAGE
- Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles .
Recombinant LIN7B protein is used in various research applications, including:
- Protein-Protein Interaction Studies: Understanding how LIN7B interacts with other proteins at the synaptic junctions.
- Cell Signaling: Investigating the role of LIN7B in cell signaling pathways.
- Synaptic Function: Studying the function of LIN7B in synaptic vesicle exocytosis and cell adhesion in the brain .
