LIN7A Human

LIN7A Human Recombinant
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In Stock
Cat. No.
BT2992
Source
Escherichia Coli.
Synonyms
Protein lin-7 homolog A, Lin-7A, hLin-7, Mammalian lin-seven protein 1, MALS-1, Tax interaction protein 33, TIP-33, Vertebrate lin-7 homolog 1, Veli-1, LIN7A, MALS1, VELI1, LIN7.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Functional Interaction Network

LIN7A operates within multiprotein complexes, particularly with:

Partner ProteinFunctionInteraction Score
CASKSynaptic scaffolding, ion channel trafficking0.999
APBA1 (Mint1)Vesicle transport, APP processing0.992
GRIN2B (NR2B)NMDA receptor localization0.937
KIF17Microtubule-based transport0.919
MPP5/PALS1Tight junction formation0.999

This network enables:

  1. Polarized trafficking of receptors (EGFR, NMDA) to specific membrane domains

  2. Synaptic vesicle exocytosis coordination via CASK interaction

  3. Neuronal migration through β-catenin-mediated recruitment

Neurodevelopmental Role

  • Embryonic expression: Detected from E13.5 in mice, increasing through corticogenesis

  • Migration defects: LIN7A knockdown delays neuronal positioning in cortical layers (60% deficient neurons in intermediate zone at P2)

  • Axonal guidance: Silencing disrupts corpus callosum formation (50% reduction in contralateral projections)

Disease Associations

12q21-deletion syndrome:

  • 14 Mb deletions encompassing LIN7A correlate with:

    • Intellectual disability (72% of cases)

    • Corpus callosum hypoplasia (83% incidence)

    • Cardiac abnormalities (67% prevalence)

Breast carcinogenesis:

  • Overexpression induces:

    • 3.2-fold increase in MCF10A invasion (p = 2.4×10⁻⁴)

    • PI3K/AKT pathway activation (2.5× phospho-AKT levels)

    • Loss of epithelial polarity in 89% of invasive micropapillary carcinomas

Experimental Models & Tools

Recombinant protein:

  • Human LIN7A (1-233 aa) expressed in E. coli:

    • 90% purity by SDS-PAGE

    • Used in synaptic complex studies

Cellular systems:

  • In utero electroporation (mouse): 65% migration defect rescue via shRNA

  • 3D mammary acini (MCF10A): LIN7A overexpression causes:

    • 230% increase in acinar size

    • 4.7-fold lumen filling

Clinical Implications Table

ConditionMechanismTherapeutic Target Potential
Intellectual disabilityImpaired NMDA receptor traffickingGRIN2B/LIN7A interaction modulators
Breast cancerPolarity loss → metastasisLIN7A-EGFR axis inhibitors
EpilepsySynaptic vesicle mislocalizationCASK/LIN7A complex stabilizers

Compiled from

Future Directions

  1. Structural studies: Cryo-EM analysis of LIN7A-CASK-APBA1 tripartite complex

  2. Therapeutic development:

    • Peptide disruptors of LIN7A-KIF17 interaction (tested in vitro)

    • CRISPR-based LIN7A restoration in 12q21-deletion models

  3. Biomarker potential: Serum LIN7A levels correlate with breast cancer metastasis (AUC = 0.82 in preliminary studies)

Product Specs

Introduction
Lin7A, a member of the lin-7 family, contains one L27 domain and one PDZ (DHR) domain. It is involved in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Lin7A is found in various tissues, including the brain, testis, kidney, placenta, and liver.
Description
Recombinant human LIN7A, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 256 amino acids (residues 1-233) with a molecular weight of 28.4 kDa. The protein includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that is sterile-filtered.
Formulation
The LIN7A protein solution (0.25 mg/ml) is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms
Protein lin-7 homolog A, Lin-7A, hLin-7, Mammalian lin-seven protein 1, MALS-1, Tax interaction protein 33, TIP-33, Vertebrate lin-7 homolog 1, Veli-1, LIN7A, MALS1, VELI1, LIN7.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMLKPSVT SAPTADMATL TVVQPLTLDR DVARAIELLE KLQESGEVPV HKLQSLKKVL QSEFCTAIRE VYQYMHETIT VNGCPEFRAR ATAKATVAAF AASEGHSHPR VVELPKTDEG LGFNVMGGKE QNSPIYISRI IPGGVAERHG GLKRGDQLLS VNGVSVEGEH HEKAVELLKA AKDSVKLVVR YTPKVLEEME ARFEKLRTAR RRQQQQLLIQ QQQQQQQQQT QQNHMS.

Product Science Overview

Structure and Domains

LIN7A comprises one L27 domain and one PDZ (DHR) domain . These domains are crucial for its function in cellular processes. The L27 domain is involved in protein-protein interactions, while the PDZ domain plays a role in binding to specific sequences in other proteins, facilitating the formation of protein complexes at the cell membrane .

Expression and Localization

LIN7A is expressed in various tissues, including the brain, testis, kidney, placenta, and liver . Its expression in these tissues suggests a role in diverse physiological processes. For instance, in the brain, LIN7A is involved in synaptic function and neuronal signaling .

Function

The primary function of LIN7A is to maintain the polarized distribution of membrane proteins. This is essential for the proper functioning of epithelial cells and neurons. By interacting with other proteins, LIN7A helps to anchor channels and receptors at specific locations on the cell membrane, ensuring that they are correctly positioned to carry out their functions .

Recombinant LIN7A

Recombinant LIN7A is produced in Escherichia coli and is a single, non-glycosylated polypeptide chain containing 256 amino acids, with a molecular mass of 28.4 kDa . It is fused to a 23 amino acid His-tag at the N-terminus, which facilitates its purification using chromatographic techniques . The recombinant protein is typically used in research to study its function and interactions with other proteins.

Applications in Research

Recombinant LIN7A is valuable in various research applications, including:

  • Protein-protein interaction studies: Understanding how LIN7A interacts with other proteins can provide insights into its role in cellular processes.
  • Functional assays: Investigating the effects of LIN7A on cell polarity and membrane protein distribution.
  • Structural studies: Analyzing the structure of LIN7A to understand the mechanisms underlying its function.
Storage and Stability

Recombinant LIN7A should be stored at 4°C if used within 2-4 weeks. For longer storage, it should be frozen at -20°C, with the addition of a carrier protein (e.g., 0.1% HSA or BSA) to prevent degradation. It is important to avoid multiple freeze-thaw cycles to maintain the protein’s stability .

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