Recombinant Proteins

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CRP (19-224 a.a) Human

c-Reactive Protein (19-224 a.a) Human Recombinant

This product consists of the recombinant human CRP protein, specifically the amino acids 19 to 224. It is produced in E. coli bacteria and is a single, non-glycosylated polypeptide chain with a molecular weight of 23.2 kDa. The purification process involves proprietary chromatographic techniques to ensure high purity.
Shipped with Ice Packs
Cat. No.
BT19251
Source
Escherichia Coli.
Appearance
The product is a clear solution that has been sterilized by filtration.

CRP Canine

C-Reactive Protein Canine Recombinant

Recombinant Canine CRP is a single polypeptide chain that contains 204 amino acids. It has a molecular weight of 23.2 kDa. This protein is glycosylated, meaning it has sugar molecules attached to it.

Shipped with Ice Packs
Cat. No.
BT19336
Source

HEK293 cells.

Appearance
White powder, filtered and freeze-dried.

CRP Human

C-Reactive Protein Human

This product consists of human CRP, derived from human plasma, with a molecular weight of 114 kDa. It serves as an indicator of inflammation and can be used to monitor and predict future coronary artery disease events.
Shipped with Ice Packs
Cat. No.
BT19418
Source
Human Plasma.
Appearance
A clear, sterile-filtered solution.

CRP Human Recombinant

c-Reactive Protein Human Recombinant

This product consists of recombinant human CRP generated in E. coli. It is a non-glycosylated polypeptide with a molecular weight of 115 kDa, reflecting its pentameric structure composed of five 23 kDa monomers. Purification is achieved through proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT19499
Source
Escherichia Coli.
Appearance
A clear, sterile-filtered solution.

CRP Mouse

C-Reactive Protein Mouse Recombinant

Recombinant CRP Mouse, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain consisting of 214 amino acids (20-225 a.a). It possesses a molecular mass of 24.1 kDa. The protein is fused to an 8 amino acid His-tag at the C-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT19573
Source

Sf9, Baculovirus cells.

Appearance
Clear, sterile-filtered solution.

CRP Rat

C-Reactive Protein Rat Recombinant

This recombinant CRP protein is produced in Sf9 insect cells. It is a single, glycosylated polypeptide chain containing 217 amino acids (20-230 a.a.) with a molecular mass of 24.1 kDa. The protein appears at approximately 28-40 kDa on SDS-PAGE due to glycosylation. It is expressed with a 6 amino acid His tag at the C-terminus for purification and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT19676
Source
Sf9, Insect cells.
Appearance
Clear, colorless, and sterile-filtered solution.
Definition and Classification

C-Reactive Protein (CRP) is an annular (ring-shaped) pentameric protein found in blood plasma, whose circulating concentrations rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells . CRP is a member of the pentraxin family of proteins .

Biological Properties

Key Biological Properties: CRP is synthesized by the liver in response to factors released by macrophages, T cells, and adipocytes . It is characterized by its pentameric structure and calcium-dependent binding to ligands like phosphocholine .

Expression Patterns and Tissue Distribution: CRP is primarily expressed in the liver, but it can also be found in other tissues such as the pancreas, gallbladder, and testicles . Its expression is highly upregulated during inflammatory conditions .

Biological Functions

Primary Biological Functions: CRP plays a crucial role in the body’s immune response. It binds to lysophosphatidylcholine expressed on the surface of dead or dying cells and some types of bacteria, thereby activating the complement system via C1q . This process helps in the clearance of pathogens and damaged cells .

Role in Immune Responses and Pathogen Recognition: CRP enhances phagocytosis by binding to pathogens and facilitating their recognition by immune cells . It also modulates inflammation by interacting with immune cells, cytokines, and endothelial cells .

Modes of Action

Mechanisms with Other Molecules and Cells: CRP activates certain parts of the immune system, specifically white blood cells called macrophages that clear foreign attackers and dead or dying cells from the body . It binds to specific pathogens and damaged cells, initiating the activation of the complement system .

Binding Partners and Downstream Signaling Cascades: CRP binds to phosphocholine on the surface of pathogens and damaged cells, which leads to the activation of the classical complement pathway . This activation results in a cascade of immune responses that help eliminate the foreign invaders .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The synthesis of CRP in hepatocytes is mainly regulated at the transcription level by cytokines such as interleukin-6 (IL-6) and interleukin-1β (IL-1β) . Transcription factors like STAT3, Rel p50, c-Rel, and C/EBPβ/δ are involved in the regulation of CRP expression .

Transcriptional Regulation and Post-Translational Modifications: CRP expression is upregulated in response to inflammatory cytokines, and its activity can be modulated by post-translational modifications .

Applications

Biomedical Research: CRP is widely used as a biomarker in biomedical research to study inflammation and immune responses .

Diagnostic Tools: CRP levels are measured in clinical settings to diagnose and monitor various inflammatory conditions, including infections, autoimmune diseases, and cardiovascular diseases .

Therapeutic Strategies: CRP is being explored as a potential therapeutic target for treating inflammatory diseases and conditions associated with elevated CRP levels .

Role in the Life Cycle

Role Throughout the Life Cycle: CRP plays a significant role in the body’s response to inflammation throughout the life cycle. Its levels can indicate the presence of inflammation during various stages of life, from development to aging and disease . Elevated CRP levels are associated with several age-related disorders and chronic inflammatory conditions .

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