Recombinant Proteins

p53
LBP
CEA
HLA
TCL
TTC
NPM
MAF
Bax
BID

HNRNPA1 Human

Heterogeneous Nuclear Ribonucleoprotein A1 Human Recombinant

Recombinant human HNRNPA1, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 343 amino acids (1-320 a.a.) with a 36.6 kDa molecular weight. A 23-amino acid His-tag is fused to the N-terminus. Purification is achieved using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT20576
Source
Escherichia Coli.
Appearance
A clear solution that has been sterilized by filtration.

HNRNPAB Human

Heterogeneous Nuclear Ribonucleoprotein A/B Human Recombinant

Recombinant human HNRNPAB, produced in E. coli bacteria, is a single polypeptide chain that does not have any sugar molecules attached (non-glycosylated). It contains 308 amino acids, specifically amino acids 1 to 285, and has a molecular weight of 33kDa. For purification and detection purposes, a 23 amino acid His-tag is attached to the N-terminus of the HNRNPAB protein. Purification is achieved using specialized chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT20674
Source
Escherichia Coli.
Appearance
A clear solution that has been sterilized by filtration.

HNRNPC Human

Heterogeneous Nuclear Ribonucleoprotein C Human Recombinant

Recombinant HNRNPC protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 313 amino acids (1-293a.a.) with a molecular mass of 34.5 kDa. It's important to note that the molecular weight observed on SDS-PAGE might be slightly higher. The protein features an N-terminal His-tag of 20 amino acids and undergoes purification using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20724
Source
Escherichia Coli.
Appearance
Clear solution, sterile filtered.

HNRNPK Human

Heterogeneous Nuclear Ribonucleoprotein K Human Recombinant

Recombinant human HNRNPK, produced in E. coli, is a single chain polypeptide comprising 299 amino acids (specifically residues 1 to 276) and exhibiting a molecular weight of 33kDa. The protein includes a 23 amino acid His-tag fused at its N-terminus. Its purification is achieved through proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT20802
Source
Escherichia Coli.
Appearance
A clear solution that has undergone sterile filtration.
Definition and Classification

Heterogeneous nuclear ribonucleoproteins (hnRNPs) are a family of RNA-binding proteins that play crucial roles in the processing of pre-mRNA into mature mRNA within the nucleus of eukaryotic cells . They are involved in various aspects of nucleic acid metabolism, including alternative splicing, mRNA stabilization, and transcriptional and translational regulation . The hnRNP family consists of over 20 members, designated alphabetically from hnRNP A1 to U, each with distinct molecular weights and functional properties .

Biological Properties

Key Biological Properties: hnRNPs are essential for the maturation, transport, and stabilization of mRNA . They bind to nascent RNA transcripts and influence their processing and export from the nucleus .

Expression Patterns: hnRNPs are ubiquitously expressed in various cell types, but their expression levels can vary depending on the tissue and developmental stage .

Tissue Distribution: hnRNPs are predominantly localized in the nucleus, although some members can shuttle between the nucleus and the cytoplasm . They are found in various tissues, including the brain, where they play critical roles in neuronal function and plasticity .

Biological Functions

Primary Biological Functions: hnRNPs are involved in multiple steps of mRNA metabolism, including alternative splicing, mRNA stabilization, and transport . They also play roles in transcriptional and translational regulation .

Role in Immune Responses and Pathogen Recognition: hnRNPs contribute to the regulation of immune responses by modulating the expression of immune-related genes . They are also involved in the recognition and response to viral infections by regulating the splicing and translation of viral RNAs .

Modes of Action

Mechanisms with Other Molecules and Cells: hnRNPs interact with various RNA molecules and other proteins to form ribonucleoprotein complexes . These interactions are crucial for their roles in RNA processing and regulation .

Binding Partners and Downstream Signaling Cascades: hnRNPs bind to specific RNA sequences and interact with other regulatory proteins to influence RNA splicing, stability, and translation . They also participate in signaling cascades that regulate gene expression and cellular responses .

Regulatory Mechanisms

Transcriptional Regulation: The expression of hnRNPs is regulated at the transcriptional level by various transcription factors and promoter elements .

Post-Translational Modifications: hnRNPs undergo several post-translational modifications, including phosphorylation, SUMOylation, ubiquitination, and methylation, which regulate their activity, localization, and interactions with other molecules .

Applications

Biomedical Research: hnRNPs are studied extensively in biomedical research due to their roles in RNA metabolism and gene regulation . They are used as models to understand RNA-binding protein functions and their implications in diseases .

Diagnostic Tools: hnRNPs serve as biomarkers for various diseases, including cancer and neurodegenerative disorders . Their expression levels and modifications can provide insights into disease states and progression .

Therapeutic Strategies: Targeting hnRNPs and their interactions with RNA is being explored as a therapeutic strategy for diseases such as cancer and viral infections . RNA-based therapies that modulate hnRNP functions are under investigation .

Role in the Life Cycle

Development to Aging and Disease: hnRNPs play critical roles throughout the life cycle, from development to aging . During development, they regulate gene expression patterns essential for cell differentiation and tissue formation . In aging and disease, dysregulation of hnRNP functions can lead to various pathologies, including neurodegenerative diseases and cancer .

© Copyright 2024 Thebiotek. All Rights Reserved.