Recombinant Proteins

p53
LBP
CEA
HLA
TCL
TTC
NPM
MAF
Bax
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EXOSC7 Human

Exosome Component 7 Human Recombinant

Recombinant Human EXOSC7, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 314 amino acids (1-291 a.a.) with a molecular weight of 34.2 kDa. It consists of a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT5835
Source
Escherichia Coli.
Appearance
A clear, colorless solution that has been sterilized by filtration.

EXOSC8 Human

Exosome Component 8 Human Recombinant

Recombinant EXOSC8 Human, expressed in E. coli, is available as a single, non-glycosylated polypeptide chain. This protein consists of 299 amino acids, with a sequence spanning from amino acid 1 to 276, and has a molecular weight of 32.4 kDa. For purification purposes, a 23 amino acid His-tag is fused to the N-terminus, and the protein is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT5887
Source
Escherichia Coli.
Appearance
Clear, colorless solution that has been sterilized by filtration.

EXOSC9 Human

Exosome Component 9 Human Recombinant

This product consists of a full-length cDNA sequence encoding the human PM/Scl 75c-beta isoform of EXOSC9. The protein has a molecular weight of 64 KDa and is fused to a hexa-histidine purification tag.
Shipped with Ice Packs
Cat. No.
BT5962
Source
Sf9 insect cells.

EXOSC10 Human

Exosome Component 10 Human Recombinant

Recombinant human EXOSC10, produced in SF9 cells, is a glycosylated polypeptide chain with a molecular weight of 102 kDa. It's expressed with a -6xHis tag and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT5651
Source
Sf9 insect cells.
Appearance
A clear, sterile-filtered solution.

EXOSC3 Human

Exosome Component 3 Human Recombinant

Recombinant EXOSC3, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 298 amino acids (specifically, amino acids 1-275). It has a molecular weight of 32.0 kDa. For purification purposes, a 23 amino acid His-tag is fused to the N-terminus of EXOSC3. The purification process involves proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT5710
Source
Escherichia Coli.
Appearance
A clear and sterile solution without any color.

EXOSC5 Human

Exosome Component 5 Human Recombinant

Recombinant human EXOSC5, produced in E. coli, is a single polypeptide chain comprising 256 amino acids (1-235) with a molecular weight of 27.5 kDa. The protein includes a 21 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT5776
Source
E.coli.
Appearance
Clear, colorless, and sterile-filtered solution.

EXOSC1 Human

Exosome Component 1 Human Recombinant

Recombinant human EXOSC1, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 218 amino acids (residues 1-195) with a molecular weight of 23.8 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT5585
Source
Escherichia Coli.
Appearance
Clear, colorless solution, sterile-filtered.
Definition and Classification

Exosomes are small extracellular vesicles (30–150 nm) released by cells into the extracellular environment. They are formed through the fusion of multivesicular bodies (MVBs) with the plasma membrane, releasing intraluminal vesicles (ILVs) into the extracellular space . Exosomes are distinct from other extracellular vesicles like apoptotic bodies and ectosomes, which are derived from cells undergoing apoptosis and plasma membrane shedding, respectively .

Biological Properties

Key Biological Properties: Exosomes carry a variety of biomolecules, including proteins, lipids, nucleic acids (DNA, RNA), and metabolites . They are involved in intercellular communication and can influence the biological properties and activities of recipient cells .

Expression Patterns and Tissue Distribution: Exosomes are secreted by a wide variety of cells, including immune cells, cancer cells, and stem cells . Their composition and function can vary depending on the cell type and physiological state of the originating cells .

Biological Functions

Primary Biological Functions: Exosomes play a crucial role in intercellular communication by transporting proteins, lipids, and nucleic acids between cells . They are involved in various physiological processes, including immune responses, tissue repair, and cell proliferation .

Role in Immune Responses and Pathogen Recognition: Exosomes can modulate immune responses by presenting antigens to immune cells and facilitating the activation of immune responses . They also play a role in pathogen recognition by transferring pathogen-derived molecules to immune cells .

Modes of Action

Mechanisms with Other Molecules and Cells: Exosomes interact with recipient cells through various mechanisms, including endocytosis, membrane fusion, and receptor-ligand interactions . They can deliver their cargo directly into the cytoplasm of recipient cells, influencing cellular functions .

Binding Partners and Downstream Signaling Cascades: Exosomes contain transmembrane proteins (e.g., CD9, CD81) that facilitate cell penetration and fusion . They also carry signaling molecules that can activate downstream signaling cascades in recipient cells, leading to changes in gene expression and cellular behavior .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The biogenesis and release of exosomes are tightly regulated by various mechanisms, including the endosomal sorting complex required for transport (ESCRT) pathway and ESCRT-independent mechanisms . These processes involve the sorting of cargo into ILVs, MVB formation, and fusion with the plasma membrane .

Transcriptional Regulation and Post-Translational Modifications: Exosome biogenesis is influenced by transcriptional regulation of genes involved in vesicle formation and secretion . Post-translational modifications, such as ubiquitination and phosphorylation, also play a role in regulating exosome release and cargo sorting .

Applications

Biomedical Research: Exosomes are valuable tools in biomedical research for studying intercellular communication and disease mechanisms .

Diagnostic Tools: Exosomes can serve as biomarkers for various diseases, including cancer, due to their specific cargo reflecting the physiological state of the originating cells .

Therapeutic Strategies: Exosomes have potential as drug delivery vehicles and cell-free therapeutic agents. They can be engineered to carry therapeutic molecules, improving bioavailability and reducing off-target effects .

Role in the Life Cycle

Role Throughout the Life Cycle: Exosomes play a role in various stages of the life cycle, from development to aging and disease . They are involved in processes such as stem cell maintenance, tissue repair, and immune modulation . Exosomes also contribute to disease progression by facilitating the spread of pathogenic molecules and promoting tumor growth .

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