Recombinant Proteins

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Product List

Y.Enterocolitica (O:8) YopM

Yersinia Enterocolitica (O:8) YopM Recombinant

Recombinant Yersinia Enterocolitica (O:8) YopM produced in E.coli is a non-glycosylated, polypeptide chain having a calculated molecular mass of 60,898 Dalton.
Y.Enterocolitica (O:8) YopM is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT26750
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

Y.Enterocolitica (O:9) LcrV

Yersinia Enterocolitica (O:9) LcrV Recombinant

Recombinant Yersinia Enterocolitica (O:9) LcrV produced in E.coli is a non-glycosylated, polypeptide chain having a calculated molecular mass of 38,668 Dalton.
Y.Enterocolitica (O:9) LcrV is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT26844
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

Y.Enterocolitica (O:9) YopB

Yersinia Enterocolitica (O:9) YopB Recombinant

Recombinant Yersinia Enterocolitica (O:9) YopB produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain having a calculated molecular mass of 43kDa.

Y.Enterocolitica (O:9) YopB is expressed with a -10x His tag at N-terminus and purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT26931
Source

Sf9, Baculovirus cells.

Appearance

Sterile Filtered clear solution.

Y.Enterocolitica (O:9) YopD

Yersinia Enterocolitica (O:9) YopD Recombinant

Recombinant Yersinia Enterocolitica (O:9) YopD produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 34,603 Dalton.
Y.Enterocolitica (O:9) YopD is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT27006
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

Y.Enterocolitica (O:9) YopE

Yersinia Enterocolitica (O:9) YopE Recombinant

Recombinant Yersinia Enterocolitica (O:9) YopE in E.coli is a non-glycosylated, polypeptide chain having a calculated molecular mass of 24kDa.

Y.Enterocolitica (O:9) YopE is expressed with a -10x His tag at N-terminus and purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT27083
Source

Escherichia Coli.

Appearance

Sterile Filtered clear solution.

Y.Enterocolitica (O:9) YopH

Yersinia Enterocolitica (O:9) YopH Recombinant

Recombinant Yersinia Enterocolitica (O:9) YopH produced in E.coli is a non-glycosylated, polypeptide chain having a calculated molecular mass of 52,311 Dalton.
Y.Enterocolitica (O:9) YopH is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT27180
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

Y.Enterocolitica (O:9) YopM

Yersinia Enterocolitica (O:9) YopM Recombinant

Recombinant Yersinia Enterocolitica (O:9) YopM produced in E.coli is a non-glycosylated, polypeptide chain having a calculated molecular mass of 42,703 Dalton.
Y.Enterocolitica (O:9) YopM is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT27259
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

Y.Enterocolitica (O:9) YopN

Yersinia Enterocolitica (O:9) YopN Recombinant

Recombinant Yersinia Enterocolitica (O:9) YopN produced in E.coli is a non-glycosylated, polypeptide chain having a calculated molecular mass of 34,296 Dalton.
Y.Enterocolitica (O:9) YopN is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT27324
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

Introduction

Definition and Classification

Yersinia enterocolitica is a Gram-negative, rod-shaped bacterium belonging to the family Yersiniaceae . It is motile at temperatures between 22–29°C but becomes non-motile at human body temperature . Y. enterocolitica is classified into six biogroups based on phenotypic characteristics and over 57 O serogroups based on their lipopolysaccharide (LPS) surface antigen . Pathogenic strains are typically found in biogroups 1B and 2-5 .

Biological Properties

Y. enterocolitica exhibits unique biological properties, including the ability to adhere to and invade host cells using surface proteins such as invasin and YadA . It is widely distributed in nature, found in the intestinal tracts of mammals, birds, and cold-blooded species . Pathogenic strains are often isolated from pigs, dogs, sheep, and environmental water sources . The expression of virulence factors like invasin is temperature-regulated, with higher expression at lower temperatures .

Biological Functions

The primary biological function of Y. enterocolitica is to cause yersiniosis, a disease characterized by diarrhea, ileitis, and mesenteric lymphadenitis . The bacterium’s cell surface structures, such as LPS and enterobacterial common antigen (ECA), play significant roles in virulence by providing adhesive properties and resistance to host immune responses . Y. enterocolitica also interacts with the host’s immune system, evading phagocytosis and inhibiting inflammatory responses .

Modes of Action

Y. enterocolitica employs several mechanisms to interact with host cells and molecules. It uses the type III secretion system (T3SS) to inject effector proteins into host cells, manipulating host cell behavior . The bacterium’s surface proteins, invasin and YadA, facilitate binding to host cells and entry . The T3SS is activated upon host cell contact and can rapidly toggle between secretion and cell division .

Regulatory Mechanisms

The expression and activity of Y. enterocolitica’s virulence factors are tightly regulated. The yop genes, which encode Yersinia outer proteins, are regulated by environmental cues through a post-transcriptional mechanism involving the yscM1 and yscM2 genes . The AraC-like regulator YbtA and the iron-responsive negative regulator Fur also play roles in regulating the bacterium’s virulence systems .

Applications

Y. enterocolitica has several applications in biomedical research, particularly in studying bacterial pathogenesis and host-pathogen interactions . Diagnostic tools for detecting Y. enterocolitica include chromogenic media and CRISPR/Cas12a-based nucleic acid identification platforms . Therapeutic strategies focus on targeting the bacterium’s virulence factors and regulatory mechanisms to prevent and control infections .

Role in the Life Cycle

Throughout its life cycle, Y. enterocolitica plays a role in various stages of development, aging, and disease. The bacterium’s ability to adhere to and invade host cells is crucial for establishing infection . Its regulatory mechanisms allow it to adapt to different environmental conditions and host immune responses . The T3SS is essential for the bacterium’s survival and replication within host cells .

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