Recombinant Proteins

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Product List

NANOG Human

NANOG Human Recombinant

NANOG Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 305 amino acids (1-305) and having a molecular mass of 34.6 kDa.
The NANOG is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT16497
Source
E.coli.
Appearance
Sterile Filtered colorless solution.

NANOG Human, His

Nanog Human Recombinant, His Tag

NANOG Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 174 amino acids (1-154) and having a molecular mass of 19.6 kDa.
The NANOG is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT16570
Source
Escherichia Coli.
Appearance
Sterile Filtered colorless solution.

NANOGP8 Human

Nanog Homeobox Pseudogene 8 Human Recombinant

NANOGP8 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 305 amino acids (1-305) and having a molecular mass of 34.6kDa.
NANOGP8 is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT16659
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

NANOG-TAT Human

NANOG-TAT Human Recombinant

NANOG Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 304 amino acids and c-terminal 13 amino acid TAT peptide having a molecular mass of 36.1kDa.
The NANOG is purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT16748
Source
Escherichia Coli.
Appearance

Sterile Filtered solution.

Introduction

Definition and Classification

NANOG is a homeobox transcription factor crucial for maintaining the pluripotency of embryonic stem cells (ESCs). It is encoded by the NANOG gene located on chromosome 12 in humans . NANOG belongs to the homeobox family of DNA-binding transcription factors, which play pivotal roles in regulating gene expression during early embryonic development .

Biological Properties

Key Biological Properties: NANOG is a master regulator of pluripotency, enabling ESCs to self-renew and maintain an undifferentiated state . It is characterized by its ability to bind DNA and regulate the expression of genes involved in maintaining stem cell properties .

Expression Patterns: NANOG is predominantly expressed in pluripotent stem cells, including ESCs and induced pluripotent stem cells (iPSCs) . Its expression is tightly regulated and diminishes as cells differentiate .

Tissue Distribution: NANOG expression is primarily restricted to early embryonic tissues and pluripotent stem cells . It is also detected in certain cancer stem cells, where it contributes to tumorigenicity .

Biological Functions

Primary Biological Functions: NANOG plays a critical role in maintaining the pluripotency and self-renewal of ESCs . It works in concert with other transcription factors such as OCT4 and SOX2 to establish and maintain the pluripotent state .

Role in Immune Responses and Pathogen Recognition: While NANOG’s primary function is related to stem cell pluripotency, its role in immune responses and pathogen recognition is not well-documented. However, its expression in certain cancer stem cells suggests a potential involvement in immune evasion mechanisms .

Modes of Action

Mechanisms with Other Molecules and Cells: NANOG interacts with various transcription factors, including OCT4 and SOX2, to regulate gene expression and maintain pluripotency . It binds to specific DNA sequences and modulates the transcription of target genes .

Binding Partners and Downstream Signaling Cascades: NANOG forms complexes with other pluripotency factors and co-regulators to orchestrate a network of gene expression that sustains the undifferentiated state of stem cells . It activates or represses downstream signaling pathways involved in cell fate determination .

Regulatory Mechanisms

Transcriptional Regulation: NANOG expression is regulated by upstream transcription factors, including OCT4 and SOX2, which bind to its promoter region and activate its transcription . Additionally, NANOG can autoregulate its expression by binding to its promoter .

Post-Translational Modifications: NANOG undergoes various post-translational modifications, such as phosphorylation and ubiquitination, which influence its stability and activity . These modifications play a crucial role in fine-tuning NANOG’s function in stem cells .

Applications

Biomedical Research: NANOG is extensively studied in the context of stem cell biology and regenerative medicine. Its role in maintaining pluripotency makes it a valuable tool for generating iPSCs and studying early embryonic development .

Diagnostic Tools: NANOG expression is used as a marker for pluripotent stem cells and certain cancer stem cells . Its detection can aid in the identification and characterization of these cell types .

Therapeutic Strategies: Targeting NANOG and its regulatory network holds potential for developing therapies aimed at eradicating cancer stem cells and improving regenerative medicine approaches .

Role in the Life Cycle

Development: NANOG is essential during early embryonic development, where it maintains the pluripotency of ESCs and ensures proper cell lineage specification . Its expression is critical for the formation of the inner cell mass in blastocysts .

Aging and Disease: While NANOG expression is primarily associated with early development, its aberrant expression in adult tissues is linked to tumorigenesis . NANOG-positive cancer stem cells contribute to tumor growth, metastasis, and resistance to therapy .

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