Recombinant Proteins

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TFPI Human, Sf9

Tissue Factor Pathway Inhibitor Human Recombinant, Sf9

Recombinant Human TFPI, expressed in Sf9 insect cells using a baculovirus expression system, is a single, glycosylated polypeptide chain. This protein consists of 285 amino acids, encompassing residues 29 to 304, with a molecular weight of 33 kDa. Notably, on SDS-PAGE analysis, the protein migrates at an apparent molecular weight of approximately 40-57 kDa due to glycosylation. The recombinant TFPI is engineered with a 9 amino acid Histidine (His) tag at the C-terminus to facilitate purification. Following expression, the protein undergoes purification using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT13647
Source

Sf9, Baculovirus cells.

Appearance
Sterile Filtered colorless solution.

TFPI2 Human

Tissue Factor Pathway Inhibitor 2 Human Recombinant

Recombinant Human TFPI2, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain comprising 199 amino acids (23-213 a.a.). It has a molecular mass of 22.9kDa and appears between 18-40kDa on SDS-PAGE under reducing conditions due to glycosylation. This TFPI2 protein is expressed with an 8 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT13742
Source
Sf9, Baculovirus cells.
Appearance
Clear, colorless solution, sterile filtered.

TFPI Human

Tissue Factor Pathway Inhibitor Human Recombinant

Recombinant Human TFPI, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 299 amino acids (residues 29-304). The protein, with a molecular weight of 34.3 kDa, incorporates a 23-amino acid His-tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT13564
Source
Escherichia Coli.
Appearance
Clear, sterile-filtered solution.
Definition and Classification

Tissue Factor Pathway Inhibitor (TFPI) is a protease inhibitor that plays a crucial role in regulating the tissue factor (TF)-initiated blood coagulation cascade. It is a single-chain polypeptide that can reversibly inhibit factor Xa (FXa) and subsequently inhibit the FVIIa-TF complex . TFPI is classified into two main isoforms: TFPIα and TFPIβ .

Biological Properties

Key Biological Properties: TFPI is a multivalent Kunitz-type proteinase inhibitor with three tandem Kunitz-type inhibitor domains . TFPIα has three Kunitz domains (K1, K2, K3) and a basic C terminus, while TFPIβ has K1 and K2 domains attached to a glycosylphosphatidyl inositol-anchored C terminus .

Expression Patterns: TFPIα is the only isoform present in platelets, whereas endothelial cells produce both isoforms, secreting TFPIα and expressing TFPIβ on the cell surface .

Tissue Distribution: TFPI is expressed in various tissues, including the liver, lungs, and placenta . It is also found in endothelial cells and platelets .

Biological Functions

Primary Biological Functions: TFPI is the primary inhibitor of the initiation of blood coagulation. It inhibits the TF-FVIIa complex and FXa, thereby modulating the severity of bleeding and clotting disorders .

Role in Immune Responses and Pathogen Recognition: TFPI also plays a role in immune responses by inhibiting TF-dependent cellular signaling, which can influence inflammatory and cancer pathologies .

Modes of Action

Mechanisms with Other Molecules and Cells: TFPI inhibits the TF-FVIIa complex and FXa through high-affinity interactions. Protein S enhances FXa inhibition by TFPIα .

Binding Partners: TFPI binds to TF, FVIIa, and FXa to form inhibitory complexes .

Downstream Signaling Cascades: TFPI suppresses TF-dependent cellular signaling, which can influence various cellular processes, including inflammation and cancer progression .

Regulatory Mechanisms

Regulatory Mechanisms: TFPI expression and activity are regulated through transcriptional and post-translational modifications. An alternative splicing event in the 5′ untranslated region allows for translational regulation of TFPIβ expression .

Transcriptional Regulation: TFPI gene expression is regulated by various transcription factors and signaling pathways .

Post-Translational Modifications: TFPI undergoes post-translational modifications, including glycosylation, which can influence its activity and stability .

Applications

Biomedical Research: TFPI is used in research to study coagulation disorders and the development of anticoagulant therapies .

Diagnostic Tools: TFPI levels can be measured to diagnose and monitor bleeding and clotting disorders .

Therapeutic Strategies: TFPI and its analogs are being explored as potential therapeutic agents for treating coagulation disorders and certain cancers .

Role in the Life Cycle

Role Throughout the Life Cycle: TFPI plays a critical role in regulating blood coagulation throughout the life cycle. It is essential for embryonic development, particularly in the central nervous system . TFPI also influences vascular development, cellular migration, and tumor angiogenesis .

From Development to Aging and Disease: TFPI’s regulatory functions are crucial from embryogenesis to aging. Dysregulation of TFPI activity can lead to various pathological conditions, including thrombosis, hemorrhage, and cancer .

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