Recombinant Proteins

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E Selectin Human

E-selectin Human Recombinant

Recombinant Human E-Selectin is produced in E. coli. This protein encompasses amino acid residues 179 to 557 and includes an amino-terminal hexahistidine tag, resulting in a total molecular weight of 45.22 kDa.
Shipped with Ice Packs
Cat. No.
BT21132
Source
Escherichia Coli.
Appearance
The product is a sterile-filtered liquid.

L Selectin Human

L-selectin Human Recombinant

Recombinant Human L-Selectin, expressed in E. coli, consists of 294 amino acids (residues 39-332) and a hexahistidine tag at the N-terminus, resulting in a 37.55 kDa protein.
Shipped with Ice Packs
Cat. No.
BT21189
Source
Escherichia Coli.
Appearance
Clear, colorless, and sterile-filtered solution.

L-Selectin Human, Sf9

L-Selectin Human Recombinant, Sf9

Recombinant L-Selectin, expressed in Sf9 insect cells using a baculovirus system, is a single, glycosylated polypeptide chain. This protein encompasses amino acids 52-345, corresponding to the extracellular domain, with a molecular weight of approximately 34.1 kDa. Due to glycosylation, it appears as a band around 40-57 kDa on SDS-PAGE. The recombinant protein includes a 6-amino acid Histidine tag at the C-terminus to facilitate purification by affinity chromatography.

Shipped with Ice Packs
Cat. No.
BT21258
Source

Sf9, Baculovirus cells.

Appearance
Clear, colorless solution, sterile-filtered.

P Selectin Human

P-selectin Human Recombinant

Recombinant Human P-Selectin is expressed in E. coli. It consists of amino acids 197-761 (566 amino acids total) fused to an amino-terminal hexahistidine tag.
Shipped with Ice Packs
Cat. No.
BT21314
Source
Escherichia Coli.
Appearance
Sterile filtered liquid.

SELE Human, HEK

E-Selectin Human Recombinant, HEK

Recombinant human SELE protein, expressed in a mammalian system using human cells, is a single polypeptide chain consisting of 543 amino acids (residues 22-556). The protein includes an 8 amino acid His-tag fused to the C-terminus and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT21392
Source
HEK293 cells.
Appearance
Sterile, white lyophilized (freeze-dried) powder.

SELE Human, Sf9

E-Selectin Human Recombinant, Sf9

This recombinant SELE protein is produced in Sf9 insect cells using a baculovirus expression system. It is a single polypeptide chain with a molecular weight of 59.4 kDa. The protein sequence includes amino acids 22 to 556 of the human SELE protein, with a 6-amino acid His-tag added to the C-terminus to facilitate purification. The protein is purified using proprietary chromatographic techniques and is glycosylated, meaning it has sugar molecules attached.

Shipped with Ice Packs
Cat. No.
BT21490
Source

Sf9, Baculovirus cells.

Appearance

The product is a colorless solution that has been sterilized by filtration.

SELPLG Human

Selectin P Ligand Human Recombinant

Recombinant Human SELPLG, produced in HEK293 cells, is a single, glycosylated polypeptide chain comprising 496 amino acids (42-295 a.a.). It has a molecular weight of 53.4 kDa. The SELPLG is fused to a 239 amino acid hIgG-His-Tag at its C-terminus and is purified using proprietary chromatographic methods.

Shipped with Ice Packs
Cat. No.
BT21579
Source

HEK293 Cells.

Appearance

Sterile filtered, colorless solution.

Definition and Classification

Selectins are a family of cell adhesion molecules (CAMs) that play a crucial role in mediating the interaction between leukocytes and endothelial cells. They are single-chain transmembrane glycoproteins that share properties with C-type lectins due to their calcium-dependent binding to sugar moieties . There are three main types of selectins:

  • L-selectin: Found on leukocytes.
  • E-selectin: Expressed on endothelial cells.
  • P-selectin: Present on platelets and endothelial cells .
Biological Properties

Selectins are characterized by their ability to bind to specific carbohydrate structures on the surfaces of cells. They have a modular structure consisting of an N-terminal lectin domain, an epidermal growth factor (EGF)-like domain, a series of consensus repeat units, a transmembrane domain, and a cytoplasmic tail .

  • Expression Patterns: L-selectin is expressed on most leukocytes, E-selectin is induced on endothelial cells by inflammatory cytokines, and P-selectin is stored in granules of platelets and endothelial cells .
  • Tissue Distribution: L-selectin is found on leukocytes, E-selectin on endothelial cells, and P-selectin on platelets and endothelial cells .
Biological Functions

Selectins are primarily involved in the initial steps of leukocyte adhesion to the endothelium, which is critical for immune responses and inflammation . They facilitate the rolling of leukocytes along the vascular endothelium, allowing them to migrate to sites of infection or injury .

  • Immune Responses: Selectins play a key role in the recruitment of leukocytes to sites of inflammation .
  • Pathogen Recognition: They help in the recognition and clearance of pathogens by mediating the interaction between leukocytes and endothelial cells .
Modes of Action

Selectins mediate their effects through binding to specific carbohydrate ligands on the surfaces of other cells . This interaction is calcium-dependent and involves the lectin domain of the selectin molecule .

  • Binding Partners: The primary ligands for selectins include sialyl Lewisx and P-selectin glycoprotein ligand-1 (PSGL-1) .
  • Downstream Signaling Cascades: Binding of selectins to their ligands triggers intracellular signaling pathways that lead to changes in cell behavior, such as increased adhesion and migration .
Regulatory Mechanisms

The expression and activity of selectins are tightly regulated at multiple levels .

  • Transcriptional Regulation: Inflammatory cytokines such as TNF-α and IL-1β induce the expression of E-selectin on endothelial cells .
  • Post-translational Modifications: Selectins undergo various post-translational modifications, including glycosylation, which are essential for their proper function .
Applications

Selectins have several applications in biomedical research and clinical practice .

  • Biomedical Research: They are used as markers to study inflammation and immune responses .
  • Diagnostic Tools: Selectins can be used as biomarkers for inflammatory diseases and certain cancers .
  • Therapeutic Strategies: Targeting selectins and their ligands is being explored as a therapeutic approach for treating inflammatory diseases and preventing cancer metastasis .
Role in the Life Cycle

Selectins play a role throughout the life cycle, from development to aging and disease .

  • Development: They are involved in the homing of lymphocytes to peripheral lymph nodes during development .
  • Aging and Disease: Dysregulation of selectin expression and function is associated with various age-related diseases, including atherosclerosis and cancer .
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