Recombinant Proteins

p53
LBP
CEA
HLA
TCL
TTC
NPM
MAF
Bax
BID

Product List

E Selectin Human

E-selectin Human Recombinant

E-Selectin Human Recombinant is expressed in E. coli containing amino acids 179-557 fused to an amino terminal hexahistidine tag, having a total molecular weight of 45.22 kDa.
Shipped with Ice Packs
Cat. No.
BT21132
Source
Escherichia Coli.
Appearance
Sterile Filtered liquid formulation.

L Selectin Human

L-selectin Human Recombinant

L-Selectin Human Recombinant is expressed in E. coli containing 294 amino acids 39-332 fused to an amino terminal hexahistidine tag, having a total molecular weight of 37.55kDa.
Shipped with Ice Packs
Cat. No.
BT21189
Source
Escherichia Coli.
Appearance
Sterile filtered colorless solution.

L-Selectin Human, Sf9

L-Selectin Human Recombinant, Sf9

L-Selectin produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 303 amino acids (52-345a.a.) and having a molecular mass of 34.1kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
L-Selectin is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT21258
Source

Sf9, Baculovirus cells.

Appearance
Sterile Filtered clear solution.

P Selectin Human

P-selectin Human Recombinant

P-Selectin Human Recombinant is expressed in E. coli containing 566 amino acids 197-761 fused to an amino terminal hexahistidine tag.
Shipped with Ice Packs
Cat. No.
BT21314
Source
Escherichia Coli.
Appearance
Sterile Filtered liquid formulation.

SELE Human, HEK

E-Selectin Human Recombinant, HEK

SELE Human Recombinant produced by mammalian expression system in human cells is a single polypeptide chain containing 543 amino acids (22-556). SELE is fused to an 8 amino acid His-tag at C-terminus is purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT21392
Source
HEK293 cells.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

SELE Human, Sf9

E-Selectin Human Recombinant, Sf9

SELE Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 541 amino acids (22-556 a.a) and having a molecular mass of 59.4kDa.
SELE is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT21490
Source

Sf9, Baculovirus cells.

Appearance

Sterile filtered colorless solution.

SELPLG Human

Selectin P Ligand Human Recombinant

SELPLG Human Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 496 amino acids (42-295 a.a) and having a molecular mass of 53.4kDa.
SELPLG is fused to a 239 amino acid hIgG-His-Tag at C-terminus & purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT21579
Source

HEK293 Cells.

Appearance

Sterile filtered colorless solution.

Introduction

Definition and Classification

Selectins are a family of cell adhesion molecules (CAMs) that play a crucial role in mediating the interaction between leukocytes and endothelial cells. They are single-chain transmembrane glycoproteins that share properties with C-type lectins due to their calcium-dependent binding to sugar moieties . There are three main types of selectins:

  • L-selectin: Found on leukocytes.
  • E-selectin: Expressed on endothelial cells.
  • P-selectin: Present on platelets and endothelial cells .
Biological Properties

Selectins are characterized by their ability to bind to specific carbohydrate structures on the surfaces of cells. They have a modular structure consisting of an N-terminal lectin domain, an epidermal growth factor (EGF)-like domain, a series of consensus repeat units, a transmembrane domain, and a cytoplasmic tail .

  • Expression Patterns: L-selectin is expressed on most leukocytes, E-selectin is induced on endothelial cells by inflammatory cytokines, and P-selectin is stored in granules of platelets and endothelial cells .
  • Tissue Distribution: L-selectin is found on leukocytes, E-selectin on endothelial cells, and P-selectin on platelets and endothelial cells .
Biological Functions

Selectins are primarily involved in the initial steps of leukocyte adhesion to the endothelium, which is critical for immune responses and inflammation . They facilitate the rolling of leukocytes along the vascular endothelium, allowing them to migrate to sites of infection or injury .

  • Immune Responses: Selectins play a key role in the recruitment of leukocytes to sites of inflammation .
  • Pathogen Recognition: They help in the recognition and clearance of pathogens by mediating the interaction between leukocytes and endothelial cells .
Modes of Action

Selectins mediate their effects through binding to specific carbohydrate ligands on the surfaces of other cells . This interaction is calcium-dependent and involves the lectin domain of the selectin molecule .

  • Binding Partners: The primary ligands for selectins include sialyl Lewisx and P-selectin glycoprotein ligand-1 (PSGL-1) .
  • Downstream Signaling Cascades: Binding of selectins to their ligands triggers intracellular signaling pathways that lead to changes in cell behavior, such as increased adhesion and migration .
Regulatory Mechanisms

The expression and activity of selectins are tightly regulated at multiple levels .

  • Transcriptional Regulation: Inflammatory cytokines such as TNF-α and IL-1β induce the expression of E-selectin on endothelial cells .
  • Post-translational Modifications: Selectins undergo various post-translational modifications, including glycosylation, which are essential for their proper function .
Applications

Selectins have several applications in biomedical research and clinical practice .

  • Biomedical Research: They are used as markers to study inflammation and immune responses .
  • Diagnostic Tools: Selectins can be used as biomarkers for inflammatory diseases and certain cancers .
  • Therapeutic Strategies: Targeting selectins and their ligands is being explored as a therapeutic approach for treating inflammatory diseases and preventing cancer metastasis .
Role in the Life Cycle

Selectins play a role throughout the life cycle, from development to aging and disease .

  • Development: They are involved in the homing of lymphocytes to peripheral lymph nodes during development .
  • Aging and Disease: Dysregulation of selectin expression and function is associated with various age-related diseases, including atherosclerosis and cancer .
© Copyright 2024 Thebiotek. All Rights Reserved.