Recombinant Proteins

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Product List

PDCD1 Human

Programmed Cell Death 1 Human Recombinant

PDCD1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 392 amino acids (21-170a.a.) and having a molecular mass of 44.0kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
PDCD1 is expressed with a 239 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT7485
Source
Sf9, Baculovirus cells.
Appearance
Sterile Filtered colorless solution.

PDCD1 Human, Active

Programmed Cell Death 1 Human Recombinant, Active

PDCD1 Human Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 383 amino acids (21-170 a.a) and having a molecular mass of 42.9kDa.
PDCD1 is fused to a 233 amino acid hIgG-His-Tag at C-terminus & purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT7553
Source

HEK293 Cells.

Appearance

Sterile filtered colorless solution.

PDCD1LG2 Human

Programmed Cell Death 1 Ligand 2 Human Recombinant

PDCD1LG2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 204 amino acids (20-200 a.a.) and having a molecular mass of 22.8kDa.
PDCD1LG2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT7689
Source
E.coli.
Appearance
Sterile Filtered colorless solution.

PDCD1LG2 Human, Sf9

Programmed Cell Death 1 Ligand 2 Human Recombinant, Sf9

PDCD1LG2 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 423 amino acids (20-200a.a.) and having a molecular mass of 47.7kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
PDCD1LG2 is expressed with a 239 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.

Shipped with Ice Packs
Cat. No.
BT7806
Source

Sf9, Baculovirus cells.

Appearance
Sterile Filtered colorless solution.

PDCD4 Human

Programmed Cell Death 4 Human Recombinant

PDCD4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 469 amino acids and having a molecular mass of 51 kDa.
Shipped with Ice Packs
Cat. No.
BT7892
Source
Escherichia Coli.
Appearance
Sterile Filtered colorless solution.

PDCD5 Human

Programmed Cell Death-5 Human Recombinant

PDCD5 Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 125 amino acids and having a molecular mass of 14 kDa.
Shipped with Ice Packs
Cat. No.
BT7998
Source
Escherichia Coli.
Appearance
Sterile Filtered colorless solution.

PDCD6 Human

Programmed Cell Death 6 Human Recombinant

PDCD6 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 211 amino acids (1-191a.a.) and having a molecular mass of 24.0 kDa.
PDCD6 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT8098
Source
Escherichia Coli.
Appearance
Sterile Filtered clear solution.

PDCD6IP Human

Programmed Cell Death 6 Interacting Protein Human Recombinant

PDCD6IP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 412 amino acids (1-392 a.a.) and having a molecular mass of 45.8 kDa. The PDCD6IP is fused to a 20 amino acid His-tag at N-terminus and purified by conventional chromatography.
Shipped with Ice Packs
Cat. No.
BT8169
Source
Escherichia Coli.
Appearance
Sterile Filtered colorless solution.

Introduction

Definition and Classification

Programmed Cell Death (PCD) refers to the regulated process by which cells undergo self-destruction in a controlled manner. This process is essential for maintaining homeostasis and proper development in multicellular organisms. PCD can be classified into several types, including:

  • Apoptosis: Often termed as cellular suicide, it involves a series of biochemical events leading to characteristic cell changes and death .
  • Autophagy: This involves the degradation of cellular components through the lysosomal machinery .
  • Necroptosis: A form of programmed necrosis that serves as a backup to apoptosis when the latter is inhibited .
Biological Properties

Key Biological Properties:

  • Expression Patterns: PCD is expressed in various tissues and is crucial during development, such as the differentiation of fingers and toes in embryos .
  • Tissue Distribution: PCD occurs in almost all tissues, playing a role in removing damaged or unnecessary cells .
Biological Functions

Primary Biological Functions:

  • Development: PCD is vital for proper tissue and organ development, such as the formation of separate digits in embryos .
  • Immune Responses: PCD helps in eliminating infected or damaged cells, thus preventing the spread of pathogens .
  • Pathogen Recognition: Cells undergoing PCD can release signals that alert the immune system to the presence of pathogens .
Modes of Action

Mechanisms:

  • Intrinsic Pathway: Triggered by internal stimuli like DNA damage, leading to the activation of pro-apoptotic proteins .
  • Extrinsic Pathway: Initiated by external signals binding to death receptors on the cell surface .
  • Binding Partners: Proteins like Bcl-2 and Bax regulate the susceptibility of cells to apoptosis .
  • Downstream Signaling Cascades: Activation of caspases, which are proteases that degrade cellular components .
Regulatory Mechanisms

Control of Expression and Activity:

  • Transcriptional Regulation: Genes like p53 play a crucial role in regulating PCD by halting the cell cycle and initiating DNA repair .
  • Post-Translational Modifications: Modifications such as phosphorylation and ubiquitination regulate the activity of proteins involved in PCD .
Applications

Biomedical Research:

  • Diagnostic Tools: Biomarkers of PCD can be used to diagnose diseases like cancer .
  • Therapeutic Strategies: Targeting PCD pathways can help in developing treatments for diseases where cell death is dysregulated, such as cancer .
Role in the Life Cycle

Throughout the Life Cycle:

  • Development: PCD is crucial during embryonic development for proper tissue and organ formation .
  • Aging: PCD helps in removing senescent cells, thus maintaining tissue homeostasis .
  • Disease: Dysregulation of PCD can lead to diseases like cancer and neurodegenerative disorders .
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