Recombinant Proteins

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SRGN Human

Serglycin Human Recombinant

Recombinant SRGN Human, produced in E.coli, is a single, non-glycosylated polypeptide chain comprising 156 amino acids (28-158) with a molecular mass of 17.4 kDa. Note that the molecular weight on SDS-PAGE might appear higher. This protein is fused to a 25 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT22168
Source
E.coli.
Appearance
Clear, colorless solution that has been sterilized by filtration.

SRGN Human, HEK

Serglycin Human Recombinant, HEK

Recombinant Human Serglycin, produced in HEK cells, is a single, glycosylated polypeptide chain. This protein comprises 137 amino acids (Tyr28-Leu158), resulting in a calculated molecular mass of 15.5 kDa. It also includes a 6 aa His tag fused at the C-terminus.
Shipped with Ice Packs
Cat. No.
BT22268
Source
HEK 293.
Appearance
Clear, colorless solution.
Definition and Classification

Serglycin, also known as hematopoietic proteoglycan core protein or secretory granule proteoglycan core protein, is a proteoglycan primarily expressed in hematopoietic cells and endothelial cells . It is encoded by the SRGN gene and is the only known intracellular proteoglycan . Proteoglycans are complex macromolecules consisting of a core protein covalently linked with glycosaminoglycan (GAG) chains .

Biological Properties

Key Biological Properties: Serglycin is unique in its ability to adopt highly divergent structures due to glycosylation with variable types of GAGs when expressed by different cell types . It is protease-resistant and rich in serine and glycine residues .

Expression Patterns and Tissue Distribution: Serglycin is predominantly expressed in hematopoietic cells, including mast cells, T lymphocytes, and macrophages . It is also found in endothelial cells and various non-hematopoietic cell types, including tumor cells .

Biological Functions

Primary Biological Functions: Serglycin plays a crucial role in the storage and secretion of bioactive molecules within secretory granules and vesicles . It is involved in the formation of storage granules in immune cells and the retention of key inflammatory mediators .

Role in Immune Responses and Pathogen Recognition: Serglycin is essential for the proper functioning of immune cells, including the storage and release of proteases, chemokines, cytokines, and growth factors . It also plays a role in granule-mediated apoptosis by serving as a scaffold for granzymes and perforin .

Modes of Action

Mechanisms with Other Molecules and Cells: Serglycin interacts with various molecules through its GAG chains or core protein . It binds to proteolytic enzymes, chemokines, cytokines, and growth factors, modulating their activities and protecting them from degradation .

Binding Partners and Downstream Signaling Cascades: Serglycin’s interactions with its binding partners can lead to the activation of downstream signaling pathways, such as Src-mediated focal adhesion turnover and cytoskeleton reorganization . These interactions facilitate processes like cell migration and immune responses .

Regulatory Mechanisms

Regulatory Mechanisms Controlling Expression and Activity: The expression of serglycin is regulated by various factors, including lipopolysaccharide (LPS) in macrophages, tumor necrosis factor (TNF) in endothelial cells, and interleukin 1β (IL-1β) in smooth muscle cells .

Transcriptional Regulation and Post-Translational Modifications: Serglycin undergoes dynamic regulation, including differences in the regulation of distinct sulfotransferases, which reflect temporal regulation important for the synthesis of granule components .

Applications

Biomedical Research: Serglycin is studied for its role in tumorigenesis, particularly in glioblastoma and non-small cell lung cancer . It is associated with poor survival in cancer patients and promotes malignant phenotypes through interactions with molecules like TGFβRI and CD44 .

Diagnostic Tools and Therapeutic Strategies: Due to its involvement in cancer progression and immune responses, serglycin is explored as a potential biomarker for cancer diagnosis and a target for therapeutic interventions .

Role in the Life Cycle

Role Throughout the Life Cycle: Serglycin plays a significant role in various stages of the life cycle, from development to aging and disease. It is crucial for the proper functioning of the immune system and the regulation of inflammatory responses . In the context of disease, serglycin’s expression and activity are altered, contributing to conditions like cancer and chronic inflammation .

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