SRGN Human

Serglycin Human Recombinant
Shipped with Ice Packs
In Stock
Cat. No.
BT22168
Source
E.coli.
Synonyms
Serglycin, PRG, PRG1, PPG, Proteoglycan 1 secretory granule, Hematopoetic proteoglycan core protein, Platelet proteoglycan core protein, proteoglycan protein core for mast cell secretory granule.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Quick Inquiry

Description

Molecular Structure

SRGN consists of a core protein with 158 amino acids and a molecular weight of ~17.4 kDa (non-glycosylated form) . Its structure includes:

  • Signal peptide (residues 1–27)

  • N-terminal domain (residues 28–76)

  • C-terminal domain (residues 77–158), rich in serine-glycine repeats for glycosaminoglycan (GAG) attachment .

Recombinant SRGN (e.g., PRO-965 by Prospec Bio) is a 156-amino acid polypeptide with an N-terminal His-tag, expressed in E. coli and purified to >90% purity .

ParameterDetailSource
Gene LocationChromosome 10q22.1
Core Protein Length158 amino acids
Molecular Weight17.4 kDa (non-glycosylated recombinant)

Biological Functions

SRGN is the only known intracellular proteoglycan and plays diverse roles:

  • Granule Formation: Mediates storage of proteases (e.g., granzyme B, elastase), cytokines, and chemokines in secretory granules .

  • Apoptosis Regulation: Scaffolds granzyme-perforin complexes in cytotoxic T-cells for targeted cell death .

  • Inflammatory Modulation: Binds and stabilizes mediators like TNF-α, IL-6, and IL-8 .

Key Interactions:

MoleculeCell TypeFunctionSource
TGFβ2TNBC cellsPromotes metastasis via EMT
PD-L1Lung adenocarcinomaEnhances immunosuppression
CXCR-2GlioblastomaDrives fibroblast activation
Carboxypeptidase A3Mast cellsMediates protease storage

Cancer Progression

  • Triple-Negative Breast Cancer (TNBC):

    • SRGN overexpression correlates with lymph node metastasis and TGFβ2-driven epithelial-mesenchymal transition (EMT) .

    • Silencing SRGN reduces invasiveness in vitro and metastasis in vivo .

  • Lung Adenocarcinoma (LUAD):

    • High SRGN levels predict poor survival (HR = 4.22) and upregulate PD-L1, promoting immune evasion .

  • Glioblastoma (GBM):

    • SRGN enhances TGFβRI signaling and fibroblast activation, fostering a pro-tumorigenic microenvironment .

Non-Cancer Pathologies

  • Diabetic Retinopathy: Plasma SRGN levels distinguish diabetic retinopathy patients from controls (AUC = 0.875) and correlate with disease severity .

  • Inflammatory Diseases: SRGN regulates neutrophil elastase storage, impacting conditions like asthma .

Diagnostic and Therapeutic Potential

ApplicationMechanismExample Study OutcomeSource
BiomarkerElevated in TNBC, LUAD, retinopathy95% specificity in retinopathy
Therapeutic TargetKnockdown reduces metastasis99% suppression in GBM models

Recombinant SRGN in Research

Prospec Bio’s recombinant SRGN (PRO-965) is widely used to study:

  • Protease binding kinetics

  • Cytokine storage mechanisms

  • In vitro tumor microenvironment modeling .

PropertyDetail
Expression SystemE. coli
Purity>90% (SDS-PAGE)
Storage-20°C with carrier protein (e.g., BSA)

Product Specs

Introduction
SRGN, a hematopoietic cell granule proteoglycan, plays a crucial role in the function of secretory granules found in various hematopoietic cells. This proteoglycan features a protease-resistant peptide core, which is essential for neutralizing harmful hydrolytic enzymes. Notably, SRGN is associated with the macromolecular complex of granzymes and perforin, acting as a key mediator in granule-mediated apoptosis.
Description
Recombinant SRGN Human, produced in E.coli, is a single, non-glycosylated polypeptide chain comprising 156 amino acids (28-158) with a molecular mass of 17.4 kDa. Note that the molecular weight on SDS-PAGE might appear higher. This protein is fused to a 25 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The SRGN solution is provided at a concentration of 1mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 2mM DTT, 0.15M NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of the SRGN protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Serglycin, PRG, PRG1, PPG, Proteoglycan 1 secretory granule, Hematopoetic proteoglycan core protein, Platelet proteoglycan core protein, proteoglycan protein core for mast cell secretory granule.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMYPTRR ARYQWVRCNP DSNSANCLEE KGPMFELLPG ESNKIPRLRT DLFPKTRIQD LNRIFPLSED YSGSGFGSGS GSGSGSGSGF LTEMEQDYQL VDESDAFHDN LRSLDRNLPS DSQDLGQHGL EEDFML.

Product Science Overview

Definition and Classification

Serglycin is a proteoglycan composed of a relatively small core protein (approximately 17 kDa) to which sulfated glycosaminoglycans (GAGs) such as heparin, heparan sulfate, or chondroitin sulfate are attached . It is primarily known as a hematopoietic cell granule proteoglycan .

Biological Properties

Serglycin is characterized by its protease-resistant peptide core, which is crucial for neutralizing hydrolytic enzymes . It is associated with the macromolecular complex of granzymes and perforin, playing a role in granule-mediated apoptosis .

Expression Patterns and Tissue Distribution

Serglycin is expressed in various cell types, particularly those of hematopoietic origin . It is stored in the secretory granules of different hematopoietic cells, including mast cells, T-lymphocytes, and neutrophils .

Biological Functions

Serglycin has several key functions:

  • Storage of Proteases: It is essential for the storage of proteases in connective tissue and mucosal mast cells, as well as granzyme B in T-lymphocytes .
  • Granule Homeostasis: It maintains the homeostasis of secretory granules in mast cells, affecting their morphology and staining properties .
  • Localization of Enzymes: Serglycin localizes neutrophil elastase in the azurophil granules of neutrophils .
Roles in Immune Responses and Pathogen Recognition

Serglycin plays a significant role in immune responses by mediating granule-mediated apoptosis and storing various proteases and enzymes that are crucial for immune functions .

Modes of Action and Mechanisms with Other Molecules and Cells

Serglycin interacts with granzymes and perforin to mediate apoptosis. It also interacts with various proteases and enzymes to facilitate their storage and function within granules .

Binding Partners and Downstream Signaling Cascades

Serglycin binds to sulfated GAGs and forms complexes with granzymes and perforin. These interactions are vital for its role in apoptosis and enzyme storage .

Regulatory Mechanisms

The expression and function of serglycin are regulated by various factors, including local growth factors such as stem cell factor (SCF) that influence mast cell development and function .

Serglycin’s multifaceted roles in immune responses, enzyme storage, and granule homeostasis make it a critical component in various physiological processes.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

SRGN Human, HEK
Serglycin Human Recombinant, HEK
Cat. No.
BT22268
Source
HEK 293.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.