Recombinant Proteins

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CNOT8 Human

CCR4-NOT Transcription Complex, Subunit 8 Human Recombinant

Recombinant human CNOT8, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 315 amino acids (residues 1-292) with a molecular weight of 35.9 kDa. It features a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic methods.
Shipped with Ice Packs
Cat. No.
BT5601
Source
Escherichia Coli.
Appearance
Clear, colorless, and sterile-filtered solution.

CNOT7 Mouse

CCR4-NOT Transcription Complex, Subunit 7 Mouse Recombinant

Recombinant CNOT7 Mouse protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 271 amino acids, with 248 amino acids (1-248 a.a) forming the CNOT7 protein and a 23 amino acid His-tag fused at the N-terminus. This protein has a molecular weight of 31.1kDa and is purified using proprietary chromatographic techniques.
Shipped with Ice Packs
Cat. No.
BT5528
Source
Escherichia Coli.
Appearance
Clear, colorless solution that has been sterilized by filtration.
Definition and Classification

The CCR4-NOT transcription complex is a multiprotein complex that plays a crucial role in gene expression regulation. It is involved in various cellular processes, including transcription, mRNA degradation, and protein modification. The complex has multiple enzymatic activities, such as poly(A) 3′-5′ exonuclease and ubiquitin ligase . It is conserved across eukaryotes and consists of several subunits, including CNOT1, CNOT2, CNOT3, CNOT4, CNOT6, CNOT7, CNOT8, CNOT9, CNOT10, and CNOT11 .

Biological Properties

The CCR4-NOT complex is highly conserved and multifunctional. It is present in both the nucleus and cytoplasm, where it regulates various aspects of mRNA metabolism . The complex is expressed in multiple tissues and has a broad tissue distribution. Key biological properties include its role in mRNA degradation, transcription initiation and elongation, ubiquitination, and protein modification .

Biological Functions

The primary biological functions of the CCR4-NOT complex include:

  • Regulation of mRNA stability and degradation: The complex is involved in deadenylation, which is the shortening of the poly(A) tail of mRNA, leading to mRNA decay .
  • Transcription regulation: It plays a role in both the activation and inhibition of transcription initiation and elongation .
  • Protein quality control: The complex is involved in ubiquitination, which tags proteins for degradation .
  • Immune responses and pathogen recognition: The complex has been implicated in the regulation of immune responses and pathogen recognition, although specific mechanisms are still being studied .
Modes of Action

The CCR4-NOT complex interacts with various molecules and cells through multiple mechanisms:

  • Binding partners: The complex interacts with RNA-binding proteins, miRNA-bound mRNAs, and ribosomes .
  • Downstream signaling cascades: It is involved in multiple signaling pathways that regulate mRNA stability, translation, and degradation .
  • Transcription elongation: The complex promotes transcription elongation by interacting with elongating RNA polymerase II complexes .
Regulatory Mechanisms

The expression and activity of the CCR4-NOT complex are regulated through several mechanisms:

  • Transcriptional regulation: The complex is regulated at the transcriptional level by various transcription factors .
  • Post-translational modifications: The complex undergoes post-translational modifications, such as ubiquitination and phosphorylation, which modulate its activity .
  • Recruitment to target mRNAs: The complex is recruited to target mRNAs through interactions with RNA-binding proteins and miRNA complexes .
Applications

The CCR4-NOT complex has several applications in biomedical research, diagnostics, and therapeutics:

  • Biomedical research: It is studied for its role in gene expression regulation and mRNA metabolism .
  • Diagnostic tools: The complex’s components can be used as biomarkers for various diseases .
  • Therapeutic strategies: Targeting the complex’s activity can be a potential therapeutic strategy for diseases related to gene expression dysregulation .
Role in the Life Cycle

The CCR4-NOT complex plays a role throughout the life cycle, from development to aging and disease:

  • Development: It is essential for embryonic development and cell differentiation .
  • Aging: The complex’s activity is linked to cellular aging processes .
  • Disease: Dysregulation of the complex is associated with various diseases, including cancer and neurodegenerative disorders .
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